Anti-RAP1A + RAP1B antibody [EPR14814]

• IP

Supplier Data

Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14814] (AB187659)

Immunoprecipitation analysis of A431 cell lysate labeling RAP1A + RAP1B with ab187659 at 1/50 dilution. Secondary staining with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgGat 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14814] (ab187659)

Predicted band size: 21 kDa

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• WB

Supplier Data

Western blot - Anti-RAP1A + RAP1B antibody [EPR14814] (AB187659)

All lanes:

Western blot - Anti-RAP1A + RAP1B antibody [EPR14814] (ab187659) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

MOLT4 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

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• WB

Supplier Data

Western blot - Anti-RAP1A + RAP1B antibody [EPR14814] (AB187659)

All lanes:

Western blot - Anti-RAP1A + RAP1B antibody [EPR14814] (ab187659) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Raw264.7 cell lysate at 10 µg

Lane 3:

PC12 cell lysate at 10 µg

Lane 4:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

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• WB

CiteAb

Western blot - Anti-RAP1A + RAP1B antibody [EPR14814] (AB187659)

RAP1A + RAP1B western blot using anti-RAP1A + RAP1B antibody [EPR14814] ab187659. Publication image and figure legend from Günes Günsel, G., Conlon, T. M., et al., 2022, Nat Commun, PubMed 35288557.

ab187659 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab187659 please see the product overview.

PRMT7 targets RAP1A expression through histone methylation.a Western blot analysis of RAP1A/RAP1B expression in Prmt7+/+ and Prmt7null MH-S cells (repeated twice). b mRNA expression level of Rap1a and Rap1b determined by qPCR in Prmt7+/+ and Prmt7null MH-S macrophage cells (n = 4 replicates per cell line). c–f ATAC-Seq analysis of Prmt7+/+ v Prmt7null MH-S cells. c ATAC-seq signal enrichment peaks around the transcription start site (TSS) of the Rap1a gene in Prmt7+/+ and Prmt7null MH-S cells and difference in peak height across the two lines. d Heat map of tag distributions across TSSs for Prmt7+/+ and Prmt7null MH-S cells. e Peak correlation scatter plot. f Pie chart showing the genomic distribution of accessible regions in Prmt7+/+ and Prmt7null MH-S cells. g Schematic representation of the location of the Rap1a regions targeted for qPCR following ChIP, plus H3K4me1 and H3K27a enrichment in BMDM (from UCSC genome browser Track accessions : wgEncodeEM002658 and wgEncodeEM002657). h Enrichment of H3R2 mono and dimethylation at Rap1a regions in Prmt7+/+ and Prmt7null MH-S by qPCR following ChIP (n = 2 per cell line). i Western blot analysis of mono and dimethylation of H3R2 in Prmt7+/+ and Prmt7null MH-S cells (repeated twice). j Dot blot depicting Rap1a expression (log-transformed, normalized UMI counts) and percentage of cells positive for Rap1a in monocytes from mouse lung single-cell RNA-seq data following exposure to FA (n = 3) and CS for 2 (n = 5) and 4 months (n = 5). k mRNA expression levels of RAP1A determined by qPCR in the monocytes of smokers (n = 10) and non-smokers (n = 11) isolated from the peripheral blood. l MFI of ITGAL and ITGAM surface expression as determined by flow cytometry in Prmt7+/+ MH-S cells incubated with 20 μM GGTI (RAP1 inhibitor) for 2 h and analysed 6 h later (n = 2, repeated three times). m Western blot analysis of phosphorylated ERK in WT MH-S cells pretreated with the RAP1 inhibitor GGTI (20 μM) for 2 h and incubated for 15 min with 1 μg/ml LPS. Quantification relative to actin shown (repeated two times). Data shown mean ± SD, P values shown in charts determined by unpaired two-tailed Student’s t-test (b, h, k, l), one-way ANOVA Bonferroni’s multiple comparisons test (m). Source data are provided as a Source Data file.

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