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Rabbit Recombinant Monoclonal RAP1B antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (AB181858), expandable thumbnail
  • Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (AB181858), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (AB181858), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function.

Additional Targets

RAP1A

Alternative names

OK/SW-cl.11, RAP1B, Ras-related protein Rap-1b, GTP-binding protein smg p21B

Recommended products

Rabbit Recombinant Monoclonal RAP1B antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14815(B)
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858), expandable thumbnail

    Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

    All lanes: Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858) at 1/50000 dilution

    Lane 1: Hela cell lysate at 20 µg

    Lane 2: A431 cell lysate at 20 µg

    Lane 3: 293 cell lysate at 20 µg

    Lane 4: MOLT4 cell lysate at 20 µg

    Secondary

    All lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858), expandable thumbnail

    Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

    Western blot analysis on immunoprecipitation from 1) 293 cell lysate or 2) negative control, labeling RAP1B using ab181858 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

    Developed using the ECL technique.

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Flow Cytometry (Intracellular) - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

    Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling RAP1B with ab181858 at a 1/30 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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