AB181858

Anti-RAP1A + RAP1B antibody [EPR14815(B)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal RAP1B antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

OK/SW-cl.11, RAP1B, Ras-related protein Rap-1b, GTP-binding protein smg p21B, KREV1, RAP1A, Ras-related protein Rap-1A, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1

3 Images

Flow Cytometry (Intracellular) - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (AB181858)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (AB181858)

Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling RAP1B with ab181858 at a 1/30 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

Supplier Data

Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (AB181858)

Western blot analysis on immunoprecipitation from 1) 293 cell lysate or 2) negative control, labeling RAP1B using ab181858 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

Predicted band size: 21 kDa

Observed band size: 21 kDa

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Supplier Data

Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (AB181858)

All lanes:

Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858) at 1/50000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

MOLT4 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

true

Carrier free

Anti-RAP1A + RAP1B antibody [EPR14815(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14815(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function.

See full target information RAP1B

Additional targets

RAP1A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 112:2349-2360 PubMed33565687

2021

Urinary malate dehydrogenase 2 is a new biomarker for early detection of non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chen Ma,Peng-Fei Tian,Zhi-Peng Chen,Dong-Sheng Yue,Cui-Cui Liu,Chen-Guang Li,Chen Chen,Hua Zhang,Hai-Lin Liu,Zhen-Fa Zhang,Liang Chen,Bin Zhang,Chang-Li Wang

Therapeutic hypothermia and temperature management 11:201-207 PubMed33709787

2021

Therapeutic Hypothermia Mitigates the Sepsis-Increased Permeability in EA. hy926 Cells by Preserving Rap1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yuehong Shen,Wu Ding

Biochimica et biophysica acta. Molecular basis of disease 1866:165962 PubMed32920118

2020

Translocase of the outer mitochondrial membrane complex subunit 20 (TOMM20) facilitates cancer aggressiveness and therapeutic resistance in chondrosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Megan E Roche,Zhao Lin,Diana Whitaker-Menezes,Tingting Zhan,Karoly Szuhai,Judith V M G Bovee,John A Abraham,Wei Jiang,Ubaldo Martinez-Outschoorn,Atrayee Basu-Mallick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAP1A + RAP1B antibody [EPR14815(B)]

Flow Cytometry (Intracellular) - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (AB181858)

Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (AB181858)

Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (AB181858)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (3)

Product promise