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Rabbit Recombinant Monoclonal RAP1A antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] - BSA and Azide free (AB250529), expandable thumbnail
  • Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] - BSA and Azide free (AB250529), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RAP1A + RAP1B antibody [EPR14815(B)] - BSA and Azide free (AB250529), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (PubMed:17916086). Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3 (PubMed:25208567). May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.

Targets

RAP1B

Alternative names

KREV1, RAP1A, Ras-related protein Rap-1A, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1

Recommended products

Rabbit Recombinant Monoclonal RAP1A antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR14815(B)

Purification technique

Affinity purification

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab250529 is the carrier-free version of Anti-RAP1A + RAP1B antibody [EPR14815(B)] ab181858.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] - BSA and Azide free (ab250529), expandable thumbnail

    Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] - BSA and Azide free (ab250529)

    This data was developed using Anti-RAP1A + RAP1B antibody [EPR14815(B)] ab181858, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (Anti-RAP1A + RAP1B antibody [EPR14815(B)] ab181858) at 1/50000 dilution

    Lane 1: Hela cell lysate at 20 µg

    Lane 2: A431 cell lysate at 20 µg

    Lane 3: 293 cell lysate at 20 µg

    Lane 4: MOLT4 cell lysate at 20 µg

    Secondary

    All lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] - BSA and Azide free (ab250529), expandable thumbnail

    Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] - BSA and Azide free (ab250529)

    This data was developed using Anti-RAP1A + RAP1B antibody [EPR14815(B)] ab181858, the same antibody clone in a different buffer formulation.

    Western blot analysis on immunoprecipitation from 1) 293 cell lysate or 2) negative control, labeling RAP1B using Anti-RAP1A + RAP1B antibody [EPR14815(B)] ab181858 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (Anti-RAP1A + RAP1B antibody [EPR14815(B)] ab181858)

    Developed using the ECL technique.

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Flow Cytometry (Intracellular) - Anti-RAP1A + RAP1B antibody [EPR14815(B)] - BSA and Azide free (ab250529), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RAP1A + RAP1B antibody [EPR14815(B)] - BSA and Azide free (ab250529)

    This data was developed using Anti-RAP1A + RAP1B antibody [EPR14815(B)] ab181858, the same antibody clone in a different buffer formulation.

    Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling RAP1B with Anti-RAP1A + RAP1B antibody [EPR14815(B)] ab181858 at a 1/30 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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