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AB154756

Anti-RAP1B antibody

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(4 Publications)

Rabbit Polyclonal RAP1B antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAP1B aa 1 to C-terminus.

View Alternative Names

OK/SW-cl.11, RAP1B, Ras-related protein Rap-1b, GTP-binding protein smg p21B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP1B antibody (AB154756)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP1B antibody (AB154756)

Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling RAP1B with ab154756 at 1/100 dilution.

Western blot - Anti-RAP1B antibody (AB154756)
  • WB

Unknown

Western blot - Anti-RAP1B antibody (AB154756)

12% SDS PAGE

All lanes:

Western blot - Anti-RAP1B antibody (ab154756) at 1/500 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RAP1B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P61224

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) .</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function.
See full target information RAP1B
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:829 PubMed40442479

2025

RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent manners.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Wang,Guangqi Yan,Hao Li,Chunyu Wang,Ye Kang,Shengli Wang,Wei Liu,Lin Lin,Renlong Zou,Kai Zeng,Manlin Wang,Ruina Luan,Baosheng Zhou,Yu Bai,Dongjun Yang,Bolin Ning,Ge Sun,Yue Zhao

Disease markers 2022:9310048 PubMed36277988

2022

lncRNA DLEU2 Accelerates Oral Cancer Progression via miR-30a-5p/RAP1B Axis to Regulate p38 MAPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbo Zhang,Yanchun Wang,Pu Xu,Yongxiu Du,Weiwei Guan

Journal of translational medicine 19:99 PubMed33676540

2021

M2 macrophage-derived exosomal microRNAs inhibit cell migration and invasion in gliomas through PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yao,Zefen Wang,Yong Cheng,Chao Ma,Yahua Zhong,Yilei Xiao,Xu Gao,Zhiqiang Li

Stem cell research & therapy 11:520 PubMed33261656

2020

Circ6401, a novel circular RNA, is implicated in repair of the damaged endometrium by Wharton's jelly-derived mesenchymal stem cells through regulation of the miR-29b-1-5p/RAP1B axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Shi,Baolan Sun,Di Wang,Yi Zhu,Xinxin Zhao,Xiaoqing Yang,Yuquan Zhang
View all publications
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Product promise

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