AB32119

Anti-RAP1GAP antibody [Y135]

RabMAb
Recombinant
What is this?

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(8 Publications)

Rabbit Recombinant Monoclonal RAP1GAP antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

KIAA0474, RAP1GA1, RAP1GAP, Rap1 GTPase-activating protein 1, Rap1GAP, Rap1GAP1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP1GAP antibody [Y135] (AB32119)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP1GAP antibody [Y135] (AB32119)

ab32119 at a dilution of 1/100 staining RAP1GA1 in paraffin embedded human stomach carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-RAP1GAP antibody [Y135] (AB32119)

Western blot : Anti-RAP1GAP antibody [Y135] ab32119 staining at 1/10000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 90 kDa in Wild-type A549 cell lysates with no signal observed at this size in RAP1GAP knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

Lanes 1 - 4:

Western blot - Anti-RAP1GAP antibody [Y135] (ab32119) at 1/10000 dilution

Lanes 1 - 4:

Western blot - Anti-RAP1GAP antibody [Y135] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/rap1gap-antibody-y135-bsa-and-azide-free-ab247232'>ab247232</a>) at 1/10000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

RAP1GAP knockout A549 at 20 µg

Lane 3:

HT-29 at 20 µg

Lane 4:

Ramos at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 90 kDa

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Western blot - Anti-RAP1GAP antibody [Y135] (AB32119)

All lanes:

Western blot - Anti-RAP1GAP antibody [Y135] (ab32119) at 1/10000 dilution

All lanes:

Jurkat cell lysate

Predicted band size: 73 kDa

Observed band size: 200 kDa,60 kDa,85-95 kDa

false

Carrier free

Anti-RAP1GAP antibody [Y135] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y135

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.

See full target information RAP1GAP

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal oncology 13:1444-1453 PubMed35837197

2022

UBE2C promotes the progression of pancreatic cancer and glycolytic activity via EGFR stabilization-mediated PI3K-Akt pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Zhu Cao,Gang Nie,Hao Hu,Xiao Zhang,Chen-Ming Ni,Zhi-Ping Huang,Guang-Lei Qiao,Liu Ouyang

Neural regeneration research 17:2472-2483 PubMed35535899

2022

ADAM10 facilitates rapid neural stem cell cycling and proper positioning within the subventricular zone niche via JAMC/RAP1Gap signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia McMillan,Gregory W Kirschen,Sanket Desai,Emma Xia,Stella E Tsirka,Adan Aguirre

Archives of Iranian medicine 25:171-177 PubMed35429959

2022

CpG Island Methylation of the Rap1Gap Gene in Medullary Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bita Faam,Ata A Ghadiri,Mohammad Ali Ghaffari,Mehdi Totonchi,Atieh Amouzegar,Fereidoun Azizi,Hajiehbibi Shahbazian,Mahmoud Hashemitabar,Seyed Ahmad Fanaei,Layasadat Khorsandi

Infectious agents and cancer 16:71 PubMed34952616

2021

The degradation of Rap1GAP via E6AP-mediated ubiquitin-proteasome pathway is associated with HPV16/18-infection in cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghui Wang,Yihang Xie,Boxuan Sun,Yuwei Guo,Ling Song,Dawit Eman Mohammednur,Chunyan Zhao

Cytogenetic and genome research 161:227-235 PubMed34311462

2021

RAP1GAP Functions as a Tumor Suppressor Gene and Is Regulated by DNA Methylation in Differentiated Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bita Faam,Mohammad A Ghaffari,Layasadat Khorsandi,Ata A Ghadiri,Mehdi Totonchi,Atieh Amouzegar,S Ahmad Fanaei,Fereidoun Azizi,Hajeih B Shahbazian,Mahmoud Hashemi Tabar

Journal of Cancer 11:3667-3674 PubMed32284763

2020

Cyclooxygenase-2 mediates gefitinib resistance in non-small cell lung cancer through the EGFR/PI3K/AKT axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qin-Fang Deng,Qi-Yu Fang,Xian-Xiu Ji,Song-Wen Zhou

Bioscience reports 40: PubMed32068236

2020

SKA3 promotes lung adenocarcinoma metastasis through the EGFR-PI3K-Akt axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Hu,Hai-Ling Chen,Li-Ming Lou,Hong Zhang,Guo-Liang Yang

BMC cell biology 19:24 PubMed30419807

2018

PIAS1 protects against myocardial ischemia-reperfusion injury by stimulating PPARγ SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Xie,Xinyu Liu,Jie Yang,Jinke Cheng,Jianmin Gu,Song Xue

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAP1GAP antibody [Y135]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP1GAP antibody [Y135] (AB32119)

Western blot - Anti-RAP1GAP antibody [Y135] (AB32119)

Western blot - Anti-RAP1GAP antibody [Y135] (AB32119)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (8)

Product promise