Anti-RAP1GAP antibody [Y135] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP1GAP antibody [Y135] - BSA and Azide free (AB247232)

This data was developed using ab32119, the same antibody clone in a different buffer formulation.

ab32119, at a dilution of 1/100, staining RAP1GA1 in paraffin embedded human stomach carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-RAP1GAP antibody [Y135] - BSA and Azide free (AB247232)

This data was developed using ab32119, the same antibody clone in a different buffer formulation.

Western blot : Anti-RAP1GAP antibody [Y135] ab32119 staining at 1/10000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 90 kDa in Wild-type A549 cell lysates with no signal observed at this size in RAP1GAP knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

Lanes 1 - 4:

Western blot - Anti-RAP1GAP antibody [Y135] (<a href='/en-us/products/primary-antibodies/rap1gap-antibody-y135-ab32119'>ab32119</a>) at 1/10000 dilution

Lanes 1 - 4:

Western blot - Anti-RAP1GAP antibody [Y135] - BSA and Azide free (ab247232) at 1/10000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

RAP1GAP knockout A549 at 20 µg

Lane 3:

HT-29 at 20 µg

Lane 4:

Ramos at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 90 kDa

false

• WB

Unknown

Western blot - Anti-RAP1GAP antibody [Y135] - BSA and Azide free (AB247232)

This data was developed using ab32119, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAP1GAP antibody [Y135] (<a href='/en-us/products/primary-antibodies/rap1gap-antibody-y135-ab32119'>ab32119</a>) at 1/10000 dilution

All lanes:

Jurkat cell lysate

Predicted band size: 73 kDa

Observed band size: 200 kDa,60 kDa,85-95 kDa

false