AB173296

Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal RAP2A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Ras-related protein Rap-2a, RbBP-30, RAP2A, Ras-related protein Rap-2c, RAP2C, Ras-related protein Rap-2b, RAP2B

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RAP2 with ab173296 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Immunofluorescent analysis of MCF7 cells labeling RAP2 with ab173296 at 1/50 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling RAP2 with ab173296 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

All lanes:

Western blot - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (ab173296) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Predicted band size: 21 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12825(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rap2A Rap2B and Rap2C are part of the Ras-related protein subfamily often called small GTPases. These proteins act as molecular switches that cycle between active GTP-bound and inactive GDP-bound states. The mass of these proteins is approximately 21 kilodaltons each. They are expressed in various tissues including the brain where they participate in signaling pathways critical for cellular communication. The three proteins share similarities in structure and function yet have unique roles in their specific cellular contexts.

Biological function summary

Rap2A Rap2B and Rap2C are involved in regulating cell migration adhesion and morphogenesis. As GTPases they interact with downstream effectors to modulate cytoskeletal dynamics and vesicle trafficking. These proteins are not part of a single complex but work collaboratively with various signaling proteins to transmit signals from the cell surface to the interior.

Pathways

Rap2 proteins play a role in the MAPK/ERK and PI3K/Akt signaling pathways. These pathways are important for cell growth survival and differentiation. They interact with proteins such as c-Raf and Akt transmitting external signals to influence cellular responses. Rap2 proteins contribute to the fine-tuning of these pathways affecting how cells respond to external stimuli.

Aberrant Rap2 signaling associates with cancer and neuronal disorders. Overexpression or mutations can lead to tumorigenic processes by affecting cell proliferation and survival mechanisms. Additionally their misregulation links to neurodegenerative conditions due to their role in neuronal signaling and plasticity. Other proteins like Ras and RhoA interact with Rap2 proteins in these pathological conditions indicating their importance in disease pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form (PubMed : 14966141, PubMed : 15342639, PubMed : 16246175, PubMed : 16540189, PubMed : 18930710, PubMed : 20159449, PubMed : 35293963). In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK (PubMed : 14966141, PubMed : 15342639, PubMed : 18930710, PubMed : 20159449). Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development (PubMed : 20159449). More generally, it is part of several signaling cascades and regulates cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading (PubMed : 14966141, PubMed : 15342639, PubMed : 16246175, PubMed : 16540189, PubMed : 18930710, PubMed : 20159449, PubMed : 35293963).

See full target information Ras-related protein Rap-2a

Additional targets

Ras-related protein Rap-2b,Ras-related protein Rap-2c

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC molecular and cell biology 26:6 PubMed39838328

2025

BRAF regulates circPSD3/miR-526b/RAP2A axis to hinder papillary thyroid carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Li,Xiaojuan Zhao,Jingge Zhao,Jing Zhao,Lemei An,Gang Wu

Oncology reports 40:179-194 PubMed29781035

2018

Knockdown of KPNA2 inhibits autophagy in oral squamous cell carcinoma cell lines by blocking p53 nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Lin,Li Gao,Zhenyu Su,Xiaofang Cao,Yuanbo Zhan,Ying Li,Bin Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Immunocytochemistry/ Immunofluorescence - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Western blot - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Product promise