Rabbit Polyclonal RAP2B antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Ras-related protein Rap-2b aa 1 to C-terminus.
Images
Publications
Filter by
- Oncology letters 21:3142021Rap2B promotes the proliferation and migration of human glioma cells via activation of the ERK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuohong Shi,Zhen ZhangPubMed 33692846
- Molecular medicine reports 23:2020lncRNA FLVCR1‑AS1 drives colorectal cancer progression via modulation of the miR‑381/RAP2A axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Han,Xiaoyan Wang,Enqiang Mao,Boyong Shen,Liang HuangPubMed 33313944
- Tumour biology : the journal of the International 35:9387-942014Rap2B promotes migration and invasion of human suprarenal epithelioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie-Hui Di et. al.PubMed 24951956
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Ras-related protein Rap-2b aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P61225
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Cow | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Zebrafish | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer pH 8.0 is recommended |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Zebrafish | Dilution info - | Notes - |
Target data
Function
Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.
Alternative names
Ras-related protein Rap-2b, RAP2B
Recommended products
Rabbit Polyclonal RAP2B antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Ras-related protein Rap-2b aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Ras-related protein Rap-2b aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P61225
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-RAP2B antibody (ab101369)
12% SDS page
All lanes: Western blot - Anti-RAP2B antibody (ab101369) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 156 kDa, 18 kDa, 183 kDa, 21 kDa, 28 kDa, 32 kDa, 38 kDa, 39 kDa, 44 kDa, 46 kDa, 47 kDa, 48 kDa, 50 kDa, 54 kDa, 65 kDa, 78 kDa, 92 kDa
Observed band size: 115 kDa, 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP2B antibody (ab101369)
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ab101369 at 1/100 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com