Rabbit Polyclonal RAP2B antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAP2B aa 1 to C-terminus.
Images
Publications
- Oncology letters 21:3142021Rap2B promotes the proliferation and migration of human glioma cells via activation of the ERK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuohong Shi,Zhen ZhangPubMed 33692846
- Molecular medicine reports 23:2020lncRNA FLVCR1‑AS1 drives colorectal cancer progression via modulation of the miR‑381/RAP2A axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Han,Xiaoyan Wang,Enqiang Mao,Boyong Shen,Liang HuangPubMed 33313944
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RAP2B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P61225
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Cow | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Zebrafish | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer pH 8.0 is recommended |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Zebrafish | Dilution info - | Notes - |
Target data
Function
Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.
Alternative names
Ras-related protein Rap-2b, RAP2B
Recommended products
Rabbit Polyclonal RAP2B antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAP2B aa 1 to C-terminus.
Alternative names
Ras-related protein Rap-2b, RAP2B
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RAP2B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P61225
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-RAP2B antibody (ab101369)
12% SDS page
All lanes: Western blot - Anti-RAP2B antibody (ab101369) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 156 kDa, 18 kDa, 183 kDa, 21 kDa, 28 kDa, 32 kDa, 38 kDa, 39 kDa, 44 kDa, 46 kDa, 47 kDa, 48 kDa, 50 kDa, 54 kDa, 65 kDa, 78 kDa, 92 kDa
Observed band size: 115 kDa, 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP2B antibody (ab101369)
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ab101369 at 1/100 dilution.
Downloads
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