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AB229277

Anti-RAP55 antibody

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(1 Publication)

Rabbit Polyclonal RAP55 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LSM14A.

View Alternative Names

C19orf13, FAM61A, RAP55, RAP55A, LSM14A, Protein LSM14 homolog A, Protein FAM61A, Protein SCD6 homolog, Putative alpha-synuclein-binding protein, RNA-associated protein 55A, AlphaSNBP, hRAP55, hRAP55A

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-RAP55 antibody (AB229277)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAP55 antibody (AB229277)

4% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for RAP55 (green) using ab229277 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue). Alpha Tubulin , a cytoskeleton marker, is detected with an alpha Tubulin antibody (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP55 antibody (AB229277)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP55 antibody (AB229277)

Paraffin-embedded rat middle brain stained for RAP55 with ab229277 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP55 antibody (AB229277)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP55 antibody (AB229277)

Paraffin-embedded mouse hindbrain stained for RAP55 with ab229277 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-RAP55 antibody (AB229277)
  • WB

Supplier Data

Western blot - Anti-RAP55 antibody (AB229277)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-RAP55 antibody (ab229277) at 1/1000 dilution

All lanes:

NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 51 kDa

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Western blot - Anti-RAP55 antibody (AB229277)
  • WB

Supplier Data

Western blot - Anti-RAP55 antibody (AB229277)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-RAP55 antibody (ab229277) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell extract at 30 µg

Lane 2:

NCI-H1299 (human lung carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HCT 116 (human colorectal carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 51 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LSM14A. The exact immunogen used to generate this antibody is proprietary information.

Q8ND56

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for formation of P-bodies, cytoplasmic structures that provide storage sites for translationally inactive mRNAs and protect them from degradation (PubMed : 16484376, PubMed : 17074753, PubMed : 29510985). Acts as a repressor of mRNA translation (PubMed : 29510985). May play a role in mitotic spindle assembly (PubMed : 26339800).
See full target information LSM14A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 13: PubMed33493132

2021

RNF168 is highly expressed in esophageal squamous cell carcinoma and contributes to the malignant behaviors in association with the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjiu Gou,Dacheng Jin,Shengliang He,Songchen Han,Qizhou Bai
View all publications
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