Rabbit Polyclonal RAP55 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human LSM14A.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LSM14A. The exact immunogen used to generate this antibody is proprietary information. Database link Q8ND56
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Rhesus monkey | Dilution info - | Notes - |
Target data
Function
Essential for formation of P-bodies, cytoplasmic structures that provide storage sites for translationally inactive mRNAs and protect them from degradation (PubMed:16484376, PubMed:17074753, PubMed:29510985). Acts as a repressor of mRNA translation (PubMed:29510985). May play a role in mitotic spindle assembly (PubMed:26339800).
Alternative names
C19orf13, FAM61A, RAP55, RAP55A, LSM14A, Protein LSM14 homolog A, Protein FAM61A, Protein SCD6 homolog, Putative alpha-synuclein-binding protein, RNA-associated protein 55A, AlphaSNBP, hRAP55, hRAP55A
Recommended products
Rabbit Polyclonal RAP55 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human LSM14A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LSM14A. The exact immunogen used to generate this antibody is proprietary information. Database link Q8ND56
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunocytochemistry/ Immunofluorescence - Anti-RAP55 antibody (ab229277)
4% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for RAP55 (green) using ab229277 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). Alpha Tubulin , a cytoskeleton marker, is detected with an alpha Tubulin antibody (red).
Western blot - Anti-RAP55 antibody (ab229277)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-RAP55 antibody (ab229277) at 1/1000 dilution
Lane 1: A549 (human lung carcinoma cell line) whole cell extract at 30 µg
Lane 2: NCI-H1299 (human lung carcinoma cell line) whole cell extract at 30 µg
Lane 3: HCT 116 (human colorectal carcinoma cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Western blot - Anti-RAP55 antibody (ab229277)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-RAP55 antibody (ab229277) at 1/1000 dilution
All lanes: NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP55 antibody (ab229277)
Paraffin-embedded mouse hindbrain stained for RAP55 with ab229277 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP55 antibody (ab229277)
Paraffin-embedded rat middle brain stained for RAP55 with ab229277 at 1/500 dilution in immunohistochemical analysis.
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