Rabbit Polyclonal RAPH1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RAPH1 aa 1100 to C-terminus.
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- Life science alliance 6:2022Loss of NDR1/2 kinases impairs endomembrane trafficking and autophagy leading to neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlavia Roşianu,Simeon R Mihaylov,Noreen Eder,Antonie Martiniuc,Suzanne Claxton,Helen R Flynn,Shamsinar Jalal,Marie-Charlotte Domart,Lucy Collinson,Mark Skehel,Ambrosius P Snijders,Matthias Krause,Sharon A Tooze,Sila K UltanirPubMed 36446521
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RAPH1 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q70E73
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes pH 6 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Recommend PFA Fixation and Triton X-100 treatment |
Target data
Function
Mediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion.
Alternative names
ALS2CR18, ALS2CR9, KIAA1681, LPD, PREL2, RMO1, RAPH1, Ras-associated and pleckstrin homology domains-containing protein 1, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein, Lamellipodin, Proline-rich EVH1 ligand 2, Protein RMO1, PREL-2
Recommended products
Rabbit Polyclonal RAPH1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RAPH1 aa 1100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RAPH1 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q70E73
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-RAPH1 antibody (ab121619)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, plasma membrane and cytoplasm. Recommended concentration of ab121619 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAPH1 antibody (ab121619)
ab121619 at 1/75 staining RALPH1 in Paraffin Embedded Human duodenum tissue. Note strong cytoplasmic positivity in glandular cells.
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