Rabbit Recombinant Monoclonal Raptor antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 29 publications.
Images
![Western blot - Anti-Raptor antibody [EP539Y] (AB40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--western-blot-img150886.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Raptor antibody [EP539Y] (AB40768)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (AB40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93966.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (AB40768)")
![Western blot - Anti-Raptor antibody [EP539Y] (AB40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--western-blot-img144627.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Raptor antibody [EP539Y] (AB40768)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (AB40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137774.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (AB40768)")
![Western blot - Anti-Raptor antibody [EP539Y] (AB40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--western-blot-img86172.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Raptor antibody [EP539Y] (AB40768)")
Publications
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- Molecular medicine reports 28:2023Iron oxide nanoparticles induce ferroptosis via the autophagic pathway by synergistic bundling with paclitaxel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Nie,Wenqing Chen,Tianmei Zhang,Shangrong Ye,Zhongyu Ren,Peng Zhang,Jian WenPubMed 37681444
- Annals of translational medicine 10:2912022Jian Pi Tiao Gan Yin alleviates obesity phenotypes through mTORC1/SREBP1 signaling and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoming Song,Lulu Han,Xiaowan Lin,Minghui Tian,Fenglei Sun,Bo FengPubMed 35433951
- Animal nutrition (Zhongguo xu mu shou yi xue hui) 10:12-182022The ubiquitin ligase Nedd4-2 mediates the regulation of PepT2 by mTORC1 in bovine mammary epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaihong Wang,Fengqi Zhao,Jianxin Liu,Hongyun LiuPubMed 35601254
- International journal of molecular medicine 48:2021Calpain 6 inhibits autophagy in inflammatory environments: A preliminary study on myoblasts and a chronic kidney disease rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Yue Zhang,Li Jie Gu,Nan Zhu,Ling Wang,Min Chao Cai,Jie Shuang Jia,Shu Rong,Wei Jie YuanPubMed 34435644
- The American journal of pathology 191:1651-16672021YAP Accelerates Notch-Driven Cholangiocarcinogenesis via mTORC1 in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinjun Lu,Baogang Peng,Ge Chen,Mario G Pes,Silvia Ribback,Cindy Ament,Hongwei Xu,Rajesh Pal,Pedro M Rodrigues,Jesus M Banales,Matthias Evert,Diego F Calvisi,Xin Chen,Biao Fan,Jingxiao WangPubMed 34129844
- Frontiers in oncology 11:5931272021Combined Anticancer Effect of Plasma-Activated Infusion and Salinomycin by Targeting Autophagy and Mitochondrial Morphology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakashi Ando,Manami Suzuki-Karasaki,Miki Suzuki-Karasaki,Jiro Ichikawa,Toyoko Ochiai,Yukihiro Yoshida,Hirotaka Haro,Yoshihiro Suzuki-KarasakiPubMed 34150606
- EMBO molecular medicine 13:e135602021A positive feedback loop between mTORC1 and cathelicidin promotes skin inflammation in rosacea.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhili Deng,Mengting Chen,Yingzi Liu,San Xu,Yuyan Ouyang,Wei Shi,Dan Jian,Ben Wang,Fangfen Liu,Jinmao Li,Qian Shi,Qinqin Peng,Ke Sha,Wenqin Xiao,Tangxiele Liu,Yiya Zhang,Hongbing Zhang,Qian Wang,Lunquan Sun,Hongfu Xie,Ji LiPubMed 33734592
- Journal of cell science 134:2020Proteomic and mechanistic dissection of the poxvirus-customized ribosome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephen DiGiuseppe et. al.PubMed 32467327
- Cell communication and signaling : CCS 18:432020Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNorihiko Sasaki et. al.PubMed 32164764
- Neoplasma 67:501-5082020Expression of Raptor and Rictor and their relationships with angiogenesis in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesF F Wen et. al.PubMed 32039630
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Expected |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes For unpurified use at 1/500. |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes For unpurified use at 1/500. |
Species Human | Dilution info 1/1000 - 1/5000 | Notes For unpurified use at 1/500. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes See . Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Component of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed:12150925, PubMed:12150926, PubMed:12747827, PubMed:24403073, PubMed:26588989, PubMed:32561715, PubMed:37541260). In response to nutrients, growth factors or amino acids, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating several substrates, such as ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) (PubMed:12150925, PubMed:12150926, PubMed:12747827, PubMed:24403073, PubMed:26588989, PubMed:37541260). In the same time, it inhibits catabolic pathways by phosphorylating the autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulators of lysosomal biogenesis and autophagy (PubMed:12150925, PubMed:12150926, PubMed:12747827, PubMed:24403073, PubMed:32561715, PubMed:37541260). The mTORC1 complex is inhibited in response to starvation and amino acid depletion (PubMed:12150925, PubMed:12150926, PubMed:12747827, PubMed:24403073, PubMed:37541260). Within the mTORC1 complex, RPTOR acts both as a molecular adapter, which (1) mediates recruitment of mTORC1 to lysosomal membranes via interaction with small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD), and a (2) substrate-specific adapter, which promotes substrate specificity by binding to TOS motif-containing proteins and direct them towards the active site of the MTOR kinase domain for phosphorylation (PubMed:12747827, PubMed:24403073, PubMed:26588989, PubMed:37541260). mTORC1 complex regulates many cellular processes, such as odontoblast and osteoclast differentiation or neuronal transmission (By similarity). mTORC1 complex in excitatory neuronal transmission is required for the prosocial behavior induced by the psychoactive substance lysergic acid diethylamide (LSD) (By similarity).
Alternative names
KIAA1303, RAPTOR, RPTOR, Regulatory-associated protein of mTOR, Raptor, p150 target of rapamycin (TOR)-scaffold protein
Recommended products
Rabbit Recombinant Monoclonal Raptor antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 29 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP539Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Raptor short for Regulatory-associated protein of mTOR functions as an important component of the mechanistic target of rapamycin complex 1 (mTORC1). Raptor itself has a molecular mass of around 150 kDa. It is expressed in many tissues with high levels found in muscle liver and brain. Raptor plays a mechanical role as a scaffolding protein which facilitates the recruitment of substrates to mTORC1 and regulates its activity. By binding with mTOR and other components Raptor ensures proper signaling involving growth factors energy levels and nutrient availability.
- Biological function summary
Raptor serves as an essential part of cell growth and proliferation by being a constituent of the mTORC1 complex. This complex is sensitive to nutrients energy oxygen and stress signals allowing cells to adjust their functions based on environmental conditions. Raptor aids mTORC1 in translating these cellular inputs into appropriate responses such as protein synthesis and autophagy inhibition. It therefore plays a pivotal role in coordinating cellular adaptation and homeostasis.
- Pathways
The mTOR signaling which involves Raptor is significant for the regulation of cell growth and metabolism. The two main pathways where Raptor shows importance are the PI3K/AKT/mTOR and AMP-activated protein kinase (AMPK) pathways. Raptor interacts with other proteins like Akt and TSC2 providing a link between growth factor signals and cellular metabolic processes. These interactions help balance energy consumption and storage maintaining cellular and organismal energy stability.
- Associated diseases and disorders
Raptor has links to cancer and type 2 diabetes. Aberrant mTORC1 activity influenced by dysregulation of Raptor contributes to tumorigenesis through uncontrolled cell proliferation. Similarly alterations in Raptor function affect insulin signaling pathways leading to metabolic disturbances observed in type 2 diabetes. In both contexts Raptor interacts with proteins like TSC1 and PRAS40 further influencing disease progression and therapeutic outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--western-blot-img150886.jpg "Western blot - Anti-Raptor antibody [EP539Y] (ab40768)")
Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
Blocking/Diluting buffer 5% NFDM /TBST
All lanes: Western blot - Anti-Raptor antibody [EP539Y] (ab40768) at 1/5000 dilution
Lane 1: HEK-293 (human embryonic kidney) whole cell lysate at 20 µg
Lane 2: HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3: MCF-7 (human breast carcinoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 140 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93966.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling Raptor with purified ab40768 at dilution of 1/100. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
![Western blot - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--western-blot-img144627.jpg "Western blot - Anti-Raptor antibody [EP539Y] (ab40768)")
Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
Blocking/Diluting buffer 5% NFDM /TBST
All lanes: Western blot - Anti-Raptor antibody [EP539Y] (ab40768) at 1/5000 dilution
All lanes: Rat brain tissue lysate at 20 mg/mL
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 140 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137774.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768)
Immunohistochemical analysis of paraffin-embedded human colon using anti-Raptor ab40768 at 1/50 dilution.
![Western blot - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--western-blot-img86172.jpg "Western blot - Anti-Raptor antibody [EP539Y] (ab40768)")
Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
Blocking/Diluting buffer 5% NFDM /TBST
All lanes: Western blot - Anti-Raptor antibody [EP539Y] (ab40768) at 1/1000 dilution
All lanes: Mouse brain tissue lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 149 kDa, 23 kDa
Observed band size: 140 kDa, 32 kDa
![Western blot - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--western-blot-img22437.jpg "Western blot - Anti-Raptor antibody [EP539Y] (ab40768)")
Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
All lanes: Western blot - Anti-Raptor antibody [EP539Y] (ab40768) at 1/500 dilution
Lane 1: 293 cell lysate at 10 µg
Lane 2: MCF-7 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: A431 cell lysate at 10 µg
Predicted band size: 149 kDa
Observed band size: 140 kDa, 21 kDa, 37 kDa
![Western blot - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--western-blot-img442682.jpg "Western blot - Anti-Raptor antibody [EP539Y] (ab40768)")
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
Raptor western blot using anti-Raptor antibody [EP539Y] ab40768. Publication image and figure legend from Chen, Z., Wang, C., et al., 2020, Mol Med, PubMed 32000660.
ab40768 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab40768 please see the product overview.
Aspirin affects phosphorylation of PI3K/Akt/Raptor pathway in CRC cells a Expression of PI3K protein and protein phosphorylation in HCT-116 cells treated with different concentrations of aspirin. b Expression of PI3K protein and protein phosphorylation in RKO cells treated with different concentrations of aspirin. c Expression of Akt protein and protein phosphorylation in HCT-116 cells treated with different concentrations of aspirin. d Expression of Akt protein and protein phosphorylation in RKO cells treated with different concentrations of aspirin. e The results of Raptor protein expression in differently treated groups in HCT-116 cells. f The results of Raptor protein expression in differently treated groups in RKO cells. All experiments were repeated 3 times, (n = 3) Mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001 (vs. normal group)
![Western blot - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--western-blot-img22437.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Raptor antibody [EP539Y] (ab40768)")
![Western blot - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail](https://content.abcam.com/products/images/raptor-antibody-ep539y-ab40768--western-blot-img442682.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Raptor antibody [EP539Y] (ab40768)")
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