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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal Raptor antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 29 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | Flow Cyt | WB | ICC/IF | IHC-P | |
---|---|---|---|---|---|
Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
Mouse | Not recommended | Not recommended | Tested | Not recommended | Expected |
Rat | Not recommended | Not recommended | Tested | Not recommended | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes For unpurified use at 1/500. |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes For unpurified use at 1/500. |
Species Human | Dilution info 1/1000 - 1/5000 | Notes For unpurified use at 1/500. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes See . Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. mTORC1 complex in excitatory neuronal transmission is required for the prosocial behavior induced by the psychoactive substance lysergic acid diethylamide (LSD) (By similarity).
Regulatory-associated protein of mTOR, Raptor, p150 target of rapamycin (TOR)-scaffold protein, RAPTOR, KIAA1303, RPTOR
Rabbit Recombinant Monoclonal Raptor antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 29 publications.
Regulatory-associated protein of mTOR, Raptor, p150 target of rapamycin (TOR)-scaffold protein, RAPTOR, KIAA1303, RPTOR
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EP539Y
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Raptor serves as an essential part of cell growth and proliferation by being a constituent of the mTORC1 complex. This complex is sensitive to nutrients energy oxygen and stress signals allowing cells to adjust their functions based on environmental conditions. Raptor aids mTORC1 in translating these cellular inputs into appropriate responses such as protein synthesis and autophagy inhibition. It therefore plays a pivotal role in coordinating cellular adaptation and homeostasis.
Raptor short for Regulatory-associated protein of mTOR functions as an important component of the mechanistic target of rapamycin complex 1 (mTORC1). Raptor itself has a molecular mass of around 150 kDa. It is expressed in many tissues with high levels found in muscle liver and brain. Raptor plays a mechanical role as a scaffolding protein which facilitates the recruitment of substrates to mTORC1 and regulates its activity. By binding with mTOR and other components Raptor ensures proper signaling involving growth factors energy levels and nutrient availability.
The mTOR signaling which involves Raptor is significant for the regulation of cell growth and metabolism. The two main pathways where Raptor shows importance are the PI3K/AKT/mTOR and AMP-activated protein kinase (AMPK) pathways. Raptor interacts with other proteins like Akt and TSC2 providing a link between growth factor signals and cellular metabolic processes. These interactions help balance energy consumption and storage maintaining cellular and organismal energy stability.
Raptor has links to cancer and type 2 diabetes. Aberrant mTORC1 activity influenced by dysregulation of Raptor contributes to tumorigenesis through uncontrolled cell proliferation. Similarly alterations in Raptor function affect insulin signaling pathways leading to metabolic disturbances observed in type 2 diabetes. In both contexts Raptor interacts with proteins like TSC1 and PRAS40 further influencing disease progression and therapeutic outcomes.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking/Diluting buffer 5% NFDM /TBST
All lanes: Western blot - Anti-Raptor antibody [EP539Y] (AB40768) at 1/5000 dilution
Lane 1: HEK-293 (human embryonic kidney) whole cell lysate at 20 µg
Lane 2: HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3: MCF-7 (human breast carcinoma) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 140 kDa
Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling Raptor with purified ab40768 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Blocking/Diluting buffer 5% NFDM /TBST
All lanes: Western blot - Anti-Raptor antibody [EP539Y] (AB40768) at 1/5000 dilution
All lanes: Rat brain tissue lysate at 20 mg/mL
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 140 kDa
Immunohistochemical analysis of paraffin-embedded human colon using anti-Raptor ab40768 at 1/50 dilution.
Blocking/Diluting buffer 5% NFDM /TBST
All lanes: Western blot - Anti-Raptor antibody [EP539Y] (AB40768) at 1/1000 dilution
All lanes: Mouse brain tissue lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 149 kDa, 23 kDa
Observed band size: 140 kDa, 32 kDa
All lanes: Western blot - Anti-Raptor antibody [EP539Y] (AB40768) at 1/500 dilution
Lane 1: 293 cell lysate at 10 µg
Lane 2: MCF-7 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: A431 cell lysate at 10 µg
Predicted band size: 149 kDa
Observed band size: 140 kDa, 21 kDa, 37 kDa
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