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Rabbit Recombinant Monoclonal Raptor antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 29 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Tested
Not recommended
Expected
Rat
Not recommended
Not recommended
Tested
Not recommended
Expected

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

-

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/5000

Notes

For unpurified use at 1/500.

Species

Rat

Dilution info

1/1000 - 1/5000

Notes

For unpurified use at 1/500.

Species

Human

Dilution info

1/1000 - 1/5000

Notes

For unpurified use at 1/500.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

See .

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. mTORC1 complex in excitatory neuronal transmission is required for the prosocial behavior induced by the psychoactive substance lysergic acid diethylamide (LSD) (By similarity).

Alternative names

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Rabbit Recombinant Monoclonal Raptor antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 29 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP539Y

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

Raptor serves as an essential part of cell growth and proliferation by being a constituent of the mTORC1 complex. This complex is sensitive to nutrients energy oxygen and stress signals allowing cells to adjust their functions based on environmental conditions. Raptor aids mTORC1 in translating these cellular inputs into appropriate responses such as protein synthesis and autophagy inhibition. It therefore plays a pivotal role in coordinating cellular adaptation and homeostasis.

Activity summary

Raptor short for Regulatory-associated protein of mTOR functions as an important component of the mechanistic target of rapamycin complex 1 (mTORC1). Raptor itself has a molecular mass of around 150 kDa. It is expressed in many tissues with high levels found in muscle liver and brain. Raptor plays a mechanical role as a scaffolding protein which facilitates the recruitment of substrates to mTORC1 and regulates its activity. By binding with mTOR and other components Raptor ensures proper signaling involving growth factors energy levels and nutrient availability.

Pathways

The mTOR signaling which involves Raptor is significant for the regulation of cell growth and metabolism. The two main pathways where Raptor shows importance are the PI3K/AKT/mTOR and AMP-activated protein kinase (AMPK) pathways. Raptor interacts with other proteins like Akt and TSC2 providing a link between growth factor signals and cellular metabolic processes. These interactions help balance energy consumption and storage maintaining cellular and organismal energy stability.

Associated diseases and disorders

Raptor has links to cancer and type 2 diabetes. Aberrant mTORC1 activity influenced by dysregulation of Raptor contributes to tumorigenesis through uncontrolled cell proliferation. Similarly alterations in Raptor function affect insulin signaling pathways leading to metabolic disturbances observed in type 2 diabetes. In both contexts Raptor interacts with proteins like TSC1 and PRAS40 further influencing disease progression and therapeutic outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail

    Western blot - Anti-Raptor antibody [EP539Y] (ab40768)

    Blocking/Diluting buffer 5% NFDM /TBST

    All lanes: Western blot - Anti-Raptor antibody [EP539Y] (AB40768) at 1/5000 dilution

    Lane 1: HEK-293 (human embryonic kidney) whole cell lysate at 20 µg

    Lane 2: HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: MCF-7 (human breast carcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 149 kDa

    Observed band size: 140 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling Raptor with purified ab40768 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Western blot - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail

    Western blot - Anti-Raptor antibody [EP539Y] (ab40768)

    Blocking/Diluting buffer 5% NFDM /TBST

    All lanes: Western blot - Anti-Raptor antibody [EP539Y] (AB40768) at 1/5000 dilution

    All lanes: Rat brain tissue lysate at 20 mg/mL

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 149 kDa

    Observed band size: 140 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768)

    Immunohistochemical analysis of paraffin-embedded human colon using anti-Raptor ab40768 at 1/50 dilution.

  • Western blot - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail

    Western blot - Anti-Raptor antibody [EP539Y] (ab40768)

    Blocking/Diluting buffer 5% NFDM /TBST

    All lanes: Western blot - Anti-Raptor antibody [EP539Y] (AB40768) at 1/1000 dilution

    All lanes: Mouse brain tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 149 kDa, 23 kDa

    Observed band size: 140 kDa, 32 kDa

  • Western blot - Anti-Raptor antibody [EP539Y] (ab40768), expandable thumbnail

    Western blot - Anti-Raptor antibody [EP539Y] (ab40768)

    All lanes: Western blot - Anti-Raptor antibody [EP539Y] (AB40768) at 1/500 dilution

    Lane 1: 293 cell lysate at 10 µg

    Lane 2: MCF-7 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: A431 cell lysate at 10 µg

    Predicted band size: 149 kDa

    Observed band size: 140 kDa, 21 kDa, 37 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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