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AB239827

Anti-Raptor antibody [EP539Y] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Raptor antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

KIAA1303, RAPTOR, RPTOR, Regulatory-associated protein of mTOR, Raptor, p150 target of rapamycin (TOR)-scaffold protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] - BSA and Azide free (AB239827)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] - BSA and Azide free (AB239827)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling Raptor with purified ab40768 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40768).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] - BSA and Azide free (AB239827)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] - BSA and Azide free (AB239827)

Immunohistochemical analysis of paraffin-embedded human colon using anti-Raptor ab40768 at 1/50 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40768).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP539Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab239827 is the carrier-free version of ab40768.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Raptor short for Regulatory-associated protein of mTOR functions as an important component of the mechanistic target of rapamycin complex 1 (mTORC1). Raptor itself has a molecular mass of around 150 kDa. It is expressed in many tissues with high levels found in muscle liver and brain. Raptor plays a mechanical role as a scaffolding protein which facilitates the recruitment of substrates to mTORC1 and regulates its activity. By binding with mTOR and other components Raptor ensures proper signaling involving growth factors energy levels and nutrient availability.
Biological function summary

Raptor serves as an essential part of cell growth and proliferation by being a constituent of the mTORC1 complex. This complex is sensitive to nutrients energy oxygen and stress signals allowing cells to adjust their functions based on environmental conditions. Raptor aids mTORC1 in translating these cellular inputs into appropriate responses such as protein synthesis and autophagy inhibition. It therefore plays a pivotal role in coordinating cellular adaptation and homeostasis.

Pathways

The mTOR signaling which involves Raptor is significant for the regulation of cell growth and metabolism. The two main pathways where Raptor shows importance are the PI3K/AKT/mTOR and AMP-activated protein kinase (AMPK) pathways. Raptor interacts with other proteins like Akt and TSC2 providing a link between growth factor signals and cellular metabolic processes. These interactions help balance energy consumption and storage maintaining cellular and organismal energy stability.

Raptor has links to cancer and type 2 diabetes. Aberrant mTORC1 activity influenced by dysregulation of Raptor contributes to tumorigenesis through uncontrolled cell proliferation. Similarly alterations in Raptor function affect insulin signaling pathways leading to metabolic disturbances observed in type 2 diabetes. In both contexts Raptor interacts with proteins like TSC1 and PRAS40 further influencing disease progression and therapeutic outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed : 12150925, PubMed : 12150926, PubMed : 12747827, PubMed : 24403073, PubMed : 26588989, PubMed : 32561715, PubMed : 37541260). In response to nutrients, growth factors or amino acids, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating several substrates, such as ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) (PubMed : 12150925, PubMed : 12150926, PubMed : 12747827, PubMed : 24403073, PubMed : 26588989, PubMed : 37541260). In the same time, it inhibits catabolic pathways by phosphorylating the autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulators of lysosomal biogenesis and autophagy (PubMed : 12150925, PubMed : 12150926, PubMed : 12747827, PubMed : 24403073, PubMed : 32561715, PubMed : 37541260). The mTORC1 complex is inhibited in response to starvation and amino acid depletion (PubMed : 12150925, PubMed : 12150926, PubMed : 12747827, PubMed : 24403073, PubMed : 37541260). Within the mTORC1 complex, RPTOR acts both as a molecular adapter, which (1) mediates recruitment of mTORC1 to lysosomal membranes via interaction with small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD), and a (2) substrate-specific adapter, which promotes substrate specificity by binding to TOS motif-containing proteins and direct them towards the active site of the MTOR kinase domain for phosphorylation (PubMed : 12747827, PubMed : 24403073, PubMed : 26588989, PubMed : 37541260). mTORC1 complex regulates many cellular processes, such as odontoblast and osteoclast differentiation or neuronal transmission (By similarity). mTORC1 complex in excitatory neuronal transmission is required for the prosocial behavior induced by the psychoactive substance lysergic acid diethylamide (LSD) (By similarity).
See full target information RPTOR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 44:1145-1162 PubMed38235747

2024

The role of serum/glucocorticoid-regulated kinase 1 in brain function following cerebral ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Celeste Yin-Chieh Wu,Yulan Zhang,Li Xu,Zhihai Huang,Peibin Zou,Garrett A Clemons,Chun Li,Cristiane T Citadin,Quanguang Zhang,Reggie Hui-Chao Lee
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Product promise

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