Rabbit Recombinant Monoclonal RARS antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-RARS antibody [EPR6298] (AB128956), expandable thumbnail](https://content.abcam.com/products/images/rars-antibody-epr6298-ab128956--western-blot-img142722.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RARS antibody [EPR6298] (AB128956)")
![Flow Cytometry (Intracellular) - Anti-RARS antibody [EPR6298] (AB128956), expandable thumbnail](https://content.abcam.com/products/images/rars-antibody-epr6298-ab128956--flow-cytometry-intracellular-img29963.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-RARS antibody [EPR6298] (AB128956)")
![OI-RD Scanning - Anti-RARS antibody [EPR6298] (AB128956), expandable thumbnail](https://content.abcam.com/products/images/rars-antibody-epr6298-ab128956--oi-rd-scanning-img95202.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-RARS antibody [EPR6298] (AB128956)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Not recommended | Predicted | Not recommended | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis (PubMed:25288775). Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1 (PubMed:17443684).
Alternative names
RARS, RARS1, Arginyl-tRNA synthetase, ArgRS
Recommended products
Rabbit Recombinant Monoclonal RARS antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR6298
- Purity
- Tissue culture supernatant
- Dissociation constant
- 4.2 x 10-12 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-RARS antibody [EPR6298] (ab128956), expandable thumbnail](https://content.abcam.com/products/images/rars-antibody-epr6298-ab128956--western-blot-img142722.jpg "Western blot - Anti-RARS antibody [EPR6298] (ab128956)")
Western blot - Anti-RARS antibody [EPR6298] (ab128956)
All lanes: Western blot - Anti-RARS antibody [EPR6298] (ab128956) at 1/1000 dilution
Lane 1: A431 cell lysate at 10 µg
Lane 2: SH-SY5Y cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: HepG2 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 75 kDa
Observed band size: 60-72 kDa
![Flow Cytometry (Intracellular) - Anti-RARS antibody [EPR6298] (ab128956), expandable thumbnail](https://content.abcam.com/products/images/rars-antibody-epr6298-ab128956--flow-cytometry-intracellular-img29963.jpg "Flow Cytometry (Intracellular) - Anti-RARS antibody [EPR6298] (ab128956)")
Flow Cytometry (Intracellular) - Anti-RARS antibody [EPR6298] (ab128956)
ab128956, at 1/10 dilution (red) staining RARS in permeabilized 293T cells, compared with a negative rabbit IgG (green) by intracellular flow cytometry.
![OI-RD Scanning - Anti-RARS antibody [EPR6298] (ab128956), expandable thumbnail](https://content.abcam.com/products/images/rars-antibody-epr6298-ab128956--oi-rd-scanning-img95202.jpg "OI-RD Scanning - Anti-RARS antibody [EPR6298] (ab128956)")
OI-RD Scanning - Anti-RARS antibody [EPR6298] (ab128956)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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