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Mouse Recombinant Monoclonal RASH antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-RAS antibody [18/Ras] (AB307738), expandable thumbnail
  • Western blot - Anti-RAS antibody [18/Ras] (AB307738), expandable thumbnail
  • Western blot - Anti-RAS antibody [18/Ras] (AB307738), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIP
Human
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).

Alternative names

HRAS1, HRAS, GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras

Recommended products

Mouse Recombinant Monoclonal RASH antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
18/Ras
Purification technique
Affinity purification Protein A
Specificity

This antibody cross-reacts with human HRAS/NRAS/KRAS.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.

Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Associated diseases and disorders

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-RAS antibody [18/Ras] (ab307738), expandable thumbnail

    Western blot - Anti-RAS antibody [18/Ras] (ab307738)

    RAS Western blot staining using mouse Anti-RAS antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody cross-reacts with human HRAS/NRAS/KRAS.

    In Western blot, anti-Ras antibody (Anti-Ras antibody [EPR18713-13] ab206969) staining at 1/1000 dilution.

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-RAS antibody [18/Ras] (ab307738) at 1/1000 dilution

    Lane 1: His-tagged human HRAS recombinant protein at 10 ng

    Lane 2: His-tagged human NRAS recombinant protein at 10 ng

    Lane 3: His-tagged human KRAS recombinant protein at 10 ng

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 21 kDa

    Exposure time: 26s

  • Western blot - Anti-RAS antibody [18/Ras] (ab307738), expandable thumbnail

    Western blot - Anti-RAS antibody [18/Ras] (ab307738)

    RAS Western blot staining using mouse Anti-RAS antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lanes 1-3 and 5: 180 seconds;Lane 4: 26 seconds.

    All lanes: Western blot - Anti-RAS antibody [18/Ras] (ab307738) at 1/1000 dilution

    Lane 1: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 4: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: SW620 (human colorectal adenocarcinoma epithelial cell)whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 21 kDa

  • Western blot - Anti-RAS antibody [18/Ras] (ab307738), expandable thumbnail

    Western blot - Anti-RAS antibody [18/Ras] (ab307738)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lanes 1-4 and 7-14: 180 seconds ;Lanes 5-6: 59 seconds.

    All lanes: Western blot - Anti-RAS antibody [18/Ras] (ab307738) at 1/100 dilution

    Lane 1: Human cerebellum tissue lysate at 10 µg

    Lane 2: Human heart tissue lysate at 10 µg

    Lane 3: Human kidney tissue lysate at 10 µg

    Lane 4: Human spleen tissue lysate at 10 µg

    Lane 5: Mouse brain tissue lysate at 10 µg

    Lane 6: Mouse kidney tissue lysate at 10 µg

    Lane 7: Rat brain tissue lysate at 10 µg

    Lane 8: Rat heart tissue lysate at 10 µg

    Lane 9: Rat kidney tissue lysate at 10 µg

    Lane 10: Rat spleen tissue lysate at 10 µg

    Lane 11: C6 (rat glial tumor glial cell) whole cell lysate at 10 µg

    Lane 12: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg

    Lane 13: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 14: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 21 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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