Anti-RAS antibody [18/Ras] - BSA and Azide free

• WB

Lab

Western blot - Anti-RAS antibody [18/Ras] - BSA and Azide free (AB307739)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab307738).

Western blot : Anti-RAS antibody [18/Ras] ab307738 staining at 1/200 dilution, shown in green; Rabbit anti-ACTN4 ab108198 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 22 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in HRAS knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW and Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-RAS antibody [18/Ras] (<a href='/en-us/products/primary-antibodies/ras-antibody-18-ras-ab307738'>ab307738</a>) at 1/200 dilution

Lane 1:

Wild-type MCF7 whole cell lysate at 20 µg

Lane 2:

HRAS knockout MCF7 whole cell lysate at 20 µg

Lane 3:

HEK-293 whole cell lysate at 20 µg

Lane 4:

HeLa whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 22 kDa

false

• WB

Supplier Data

Western blot - Anti-RAS antibody [18/Ras] - BSA and Azide free (AB307739)

This data was produced using ab307738, the same antibody clone but in a different buffer. Blocking and diluting buffer and concentration : 5% NFDM/TBST. This antibody cross-reacts with human HRAS/NRAS/KRAS. In Western blot, anti-Ras antibody (ab206969) staining at 1/1000 dilution. Exposure time : 26 seconds.

All lanes:

Western blot - Anti-RAS antibody [18/Ras] (<a href='/en-us/products/primary-antibodies/ras-antibody-18-ras-ab307738'>ab307738</a>) at 1/1000 dilution

Lane 1:

His-tagged human HRAS recombinant protein at 10 ng

Lane 2:

His-tagged human NRAS recombinant protein at 10 ng

Lane 3:

His-tagged human KRAS recombinant protein at 10 ng

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 21 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-RAS antibody [18/Ras] - BSA and Azide free (AB307739)

This data was produced using ab307738, the same antibody clone but in a different buffer. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : Lanes 1-3 and 5 : 180 seconds;Lane 4 : 26 seconds.

All lanes:

Western blot - Anti-RAS antibody [18/Ras] (<a href='/en-us/products/primary-antibodies/ras-antibody-18-ras-ab307738'>ab307738</a>) at 1/1000 dilution

Lane 1:

HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

SW620 (human colorectal adenocarcinoma epithelial cell)whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-RAS antibody [18/Ras] - BSA and Azide free (AB307739)

This data was produced using ab307738, the same antibody clone but in a different buffer. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : Lanes 1-4 and 7-14 : 180 seconds;Lanes 5-6 : 59 seconds.

All lanes:

Western blot - Anti-RAS antibody [18/Ras] (<a href='/en-us/products/primary-antibodies/ras-antibody-18-ras-ab307738'>ab307738</a>) at 1/100 dilution

Lane 1:

Human cerebellum tissue lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Lane 3:

Human kidney tissue lysate at 10 µg

Lane 4:

Human spleen tissue lysate at 10 µg

Lane 5:

Mouse brain tissue lysate at 10 µg

Lane 6:

Mouse kidney tissue lysate at 10 µg

Lane 7:

Rat brain tissue lysate at 10 µg

Lane 8:

Rat heart tissue lysate at 10 µg

Lane 9:

Rat kidney tissue lysate at 10 µg

Lane 10:

Rat spleen tissue lysate at 10 µg

Lane 11:

C6 (rat glial tumor glial cell) whole cell lysate at 10 µg

Lane 12:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg

Lane 13:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 14:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 21 kDa

false