AB180772

Anti-Ras antibody

Name: Anti-Ras antibody (ab180772) | Abcam
Brand: Abcam Limited
SKU: AB180772
Price: 470 USD
Availability: InStock
Rating: 5 (7 reviews)

(7 Reviews)

(50 Publications)

Rabbit Polyclonal RASK antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 50 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KRAS aa 1-200.

View Alternative Names

KRAS2, RASK2, KRAS, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody (AB180772)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody (AB180772)

Immunofluorescence staining of C6 cells stained for RAS with ab180772 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

Supplier Data

Western blot - Anti-Ras antibody (AB180772)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Ras antibody (ab180772) at 1/1000 dilution

Lane 1:

A-549 cell extract at 25 µg

Lane 2:

HeLa cell extract at 25 µg

Lane 3:

Raji cell extract at 25 µg

Lane 4:

Mouse spleen tissue extract at 25 µg

Lane 5:

Mouse lung tissue extract at 25 µg

Lane 6:

Rat brain tissue extract at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG

Predicted band size: 21 kDa

true

Exposure time: 90s

CiteAb

Western blot - Anti-Ras antibody (AB180772)

Ras western blot using anti-Ras antibody ab180772. Publication image and figure legend from Wu, Y. H., Huang, Y. F., et al., 2020, Front Pharmacol, PubMed 32194423.

ab180772 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab180772 please see the product overview.

AZD8835 downregulates BRCA1/BRCA2 mRNA expression in KRAS-mutated OVCAR-8 cell via ERK signaling. (A) The mRNA expression levels of BRCA1 and BRCA2 in OVCAR-8 cells treated with different concentrations of AZD compounds for 24 h were evaluated by real-time RT-PCR. All experiments were performed in triplicate. (B) Protein expression levels of p-Akt (S473), p-Akt (T308), Akt, KRAS, p-C-Raf, ERK, and p-ERK in OVCAR-8 cells treated with different concentrations of AZD compounds for 24 h were evaluated by western blot. The β-actin protein was used as an internal loading control. All experiments were performed in triplicate. (C) Protein expression levels of p-Akt (S473), p-Akt (T308), Akt, KRAS, p-C-Raf, ERK, and p-ERK in OVCAR-3, OVCAR-4, and OVCAR-8 cells treated with different concentrations of AZD8835 for 24 h were evaluated by western blot. The β-actin protein was used as an internal loading control. All experiments were performed in triplicate. (D) In the presence of KRAS mutation, AZD8835 inhibited BRCA1/2 expression through ERK signaling.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human KRAS aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P01116

Specificity

The immunogen used for this product shares 82% homology with HRAS and NRAS. Cross-reactivity with these proteins has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.05% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.

Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed : 20949621, PubMed : 39809765). Plays an important role in the regulation of cell proliferation (PubMed : 22711838, PubMed : 23698361). Activates MAPK1/MAPK3 resulting in phosphorylation and ultimately degradation of GJA1 (By similarity). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed : 24623306).

See full target information KRAS

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Publications (50)

Recent publications for all applications. Explore the full list and refine your search

Natural products and bioprospecting 14:58 PubMed39495380

2024

Dayuan Yin alleviates symptoms of HCoV-229E-induced pneumonia and modulates the Ras/Raf1/MEK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Li,Wen Zhang,Bei Huang,Guotong Sun,Yifei Xie,Junke Song,Shumei Wang,Guanhua Du

Journal of inflammation research 17:1687-1706 PubMed38504693

2024

Envoplakin Inhibits Macrophage Polarization by Altering the Inflammatory Tumor Microenvironment of Melanoma Through the RAS / ERK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weilin Cai,Minliang Chen

Oncology reports 51: PubMed38131227

2023

Rab25 suppresses colon cancer cell invasion through upregulating claudin‑7 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Su Jin Cho,Bo Young Jeong,Se-Hee Yoon,Chang Gyo Park,Hoi Young Lee

Cancer research communications 3:2623-2639 PubMed38051103

2023

Discovery of KRB-456, a KRAS G12D Switch-I/II Allosteric Pocket Binder That Inhibits the Growth of Pancreatic Cancer Patient-derived Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Aslamuzzaman Kazi,Alok Ranjan,Vasantha Kumar M V,Bogos Agianian,Martin Garcia Chavez,Vignesh Vudatha,Rui Wang,Rajanikanth Vangipurapu,Liwei Chen,Perry Kennedy,Karthikeyan Subramanian,Jonathan C K Quirke,Francisca Beato,Patrick W Underwood,Jason B Fleming,Jose Trevino,Paul J Hergenrother,Evripidis Gavathiotis,Said M Sebti

JCI insight 8: PubMed37937641

2023

SLC25A21 downregulation promotes KRAS-mutant colorectal cancer progression by increasing glutamine anaplerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sha-Sha Hu,Yue Han,Tian-Yuan Tan,Hui Chen,Jia-Wen Gao,Lan Wang,Min-Hui Yang,Li Zhao,Yi-Qing Wang,Yan-Qing Ding,Shuang Wang

Heliyon 9:e17120 PubMed37360090

2023

Circular RNA circBNC2 facilitates glycolysis and stemness of hepatocellular carcinoma through the miR-217/high mobility group AT-hook 2 (HMGA2) axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Feng,Shufeng Xia,Junlan Hui,Yan Xu

Environmental toxicology 38:1968-1979 PubMed37186036

2023

EPN3 plays oncogenic role in non-small cell lung cancer by activating the JAK1/2-STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiguang Meng,Chunyang Zhang,Nengyang Zhu,Caiyun Zhang,Meng Liu,Zhihai Han,Yongqun Li

Cell discovery 9:20 PubMed36810288

2023

MTSS1 curtails lung adenocarcinoma immune evasion by promoting AIP4-mediated PD-L1 monoubiquitination and lysosomal degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Wang,Zhenchang Jia,Chenxi Liang,Yunfei He,Min Cong,Qiuyao Wu,Pu Tian,Dasa He,Xiang Miao,Beibei Sun,Yue Yin,Chao Peng,Feng Yao,Da Fu,Yajun Liang,Peiyuan Zhang,Hua Xiong,Guohong Hu

Cell research 33:30-45 PubMed36241718

2022

A novel protein RASON encoded by a lncRNA controls oncogenic RAS signaling in KRAS mutant cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Rongjie Cheng,Fanying Li,Maolei Zhang,Xin Xia,Jianzhuang Wu,Xinya Gao,Huangkai Zhou,Zhi Zhang,Nunu Huang,Xuesong Yang,Yaliang Zhang,Shunli Shen,Tiebang Kang,Zexian Liu,Feizhe Xiao,Hongwei Yao,Jianbo Xu,Chao Yan,Nu Zhang

Molecular cancer 21:125 PubMed35681235

2022

CBX2 shapes chromatin accessibility promoting AML via p38 MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nunzio Del Gaudio,Antonella Di Costanzo,Ning Qing Liu,Lidio Conte,Carmela Dell'Aversana,Guglielmo Bove,Rosaria Benedetti,Liliana Montella,Fortunato Ciardiello,Vincenzo Carafa,Concetta Ambrosino,Valeria Tucci,Mariarosaria Conte,Joost H A Martens,Hendrik G Stunnenberg,Angela Nebbioso,Lucia Altucci

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ras antibody

Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody (AB180772)

Western blot - Anti-Ras antibody (AB180772)

Western blot - Anti-Ras antibody (AB180772)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (50)

Product promise