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AB69747

Anti-Ras antibody

4

(10 Reviews)

|

(5 Publications)

Rabbit Polyclonal RASH antibody. Suitable for WB and reacts with Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human HRAS conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HRAS1, HRAS, GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras

1 Images
Western blot - Anti-Ras antibody (AB69747)
  • WB

Unknown

Western blot - Anti-Ras antibody (AB69747)

All lanes:

Western blot - Anti-Ras antibody (ab69747) at 1 µg/mL

All lanes:

rat brain tissue lysate

Predicted band size: 21 kDa

Observed band size: 19 kDa,21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human HRAS conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P01112

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Alternative Products

Primary Antibodies

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Primary Antibodies

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Western blot - HRP Anti-Ras antibody [EPR3255] (AB199557)

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Primary Antibodies

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Primary Antibodies

AB209974

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Flow Cytometry (Intracellular) - Anti-Ras antibody [EPR3255] - BSA and Azide free (AB209974)

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Complementary Products

Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

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Primary Antibodies

AB15580

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.
Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the activation of Ras protein signal transduction (PubMed : 22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed : 12740440, PubMed : 14500341, PubMed : 9020151).
See full target information HRAS
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 31:881-888 PubMed35394130

2022

mGluR5 promotes the progression of multiple myeloma in vitro via Ras-MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Kou,Haibin Huang,Shuangwu Dai,Xinmei Tan,Qi Chen,Renwei Huang,Hequn Zou

Oncology letters 21:481 PubMed33968197

2021

miR-188-5p promotes oxaliplatin resistance by targeting RASA1 in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xijia Zhu,Xishun Luo,Zhike Song,Shiyu Jiang,Xiangkai Long,Xueyuan Gao,Xinyang Xie,Laijian Zheng,Haipeng Wang

eLife 10: PubMed33526168

2021

A small-molecule ICMT inhibitor delays senescence of Hutchinson-Gilford progeria syndrome cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Chen,Haidong Yao,Muhammad Kashif,Gwladys Revêchon,Maria Eriksson,Jianjiang Hu,Ting Wang,Yiran Liu,Elin Tüksammel,Staffan Strömblad,Ian M Ahearn,Mark R Philips,Clotilde Wiel,Mohamed X Ibrahim,Martin O Bergo

Cell cycle (Georgetown, Tex.) 19:2611-2621 PubMed32897806

2020

Long non-coding RNA GASL1 restrains gastric carcinoma cell proliferation and metastasis by sponging microRNA-106a.

Applications

Unspecified application

Species

Unspecified reactive species

Dengqiang Liu,Peng Xiao,Chao Feng,Hui Meng,Enxu Bi

Cell cycle (Georgetown, Tex.) 12:2510-7 PubMed23839032

2013

Cyclin D1 localizes in the cytoplasm of keratinocytes during skin differentiation and regulates cell-matrix adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Fernández-Hernández,Marta Rafel,Noel P Fusté,Rafael S Aguayo,Josep M Casanova,Joaquim Egea,Francisco Ferrezuelo,Eloi Garí
View all publications
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Product promise

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