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Rabbit Recombinant Monoclonal RASH antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 75 publications.


Images

Immunoprecipitation - Anti-Ras antibody [EP1125Y] (AB52939), expandable thumbnail
  • Western blot - Anti-Ras antibody [EP1125Y] (AB52939), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Ras antibody [EP1125Y] (AB52939), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] (AB52939), expandable thumbnail
  • Western blot - Anti-Ras antibody [EP1125Y] (AB52939), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Expected
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/20

Notes

For unpurified use at 1/30.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/5000

Notes

For unpurified use at 1/10000- 1/50000.

Species

Rat

Dilution info

1/5000

Notes

For unpurified use at 1/10000- 1/50000.

Species

Human

Dilution info

1/5000

Notes

For unpurified use at 1/10000- 1/50000.

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/30

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100.

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

1 product for Alternative Version

Target data

Function

Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).

Alternative names

HRAS1, HRAS, GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras

Recommended products

Rabbit Recombinant Monoclonal RASH antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 75 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1125Y

Purification technique

Affinity purification Protein A

Specificity

This antibody is predicted to react with H-Ras, N-Ras and K-Ras.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.

Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Associated diseases and disorders

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-Ras antibody [EP1125Y] (ab52939), expandable thumbnail

    Immunoprecipitation - Anti-Ras antibody [EP1125Y] (ab52939)

    ab52939 (purified) at 1:20 dilution (2μg) immunoprecipitating Ras in Jurkat whole cell lysate.

    Lane 1 (input): Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate 10μg
    Lane 2 (+): ab52939 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52939 in Jurkat whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Ras antibody [EP1125Y] (ab52939)

    Predicted band size: 21 kDa

  • Western blot - Anti-Ras antibody [EP1125Y] (ab52939), expandable thumbnail

    Western blot - Anti-Ras antibody [EP1125Y] (ab52939)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Ras antibody [EP1125Y] (ab52939) at 1/5000 dilution

    Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysates at 20 µg

    Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysates at 20 µg

    Lane 3: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysates at 20 µg

    Lane 4: Neuro-2a (mouse neuroblastoma cell line) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Flow Cytometry (Intracellular) - Anti-Ras antibody [EP1125Y] (ab52939), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ras antibody [EP1125Y] (ab52939)

    Intracellular Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma cell line) cells labeling Ras with purified ab52939 at 1/30 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] (ab52939), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] (ab52939)

    Immunocytochemistry analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling Ras with Purified ab52939 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Ras antibody [EP1125Y] (ab52939), expandable thumbnail

    Western blot - Anti-Ras antibody [EP1125Y] (ab52939)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Ras antibody [EP1125Y] (ab52939) at 1/5000 dilution

    All lanes: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa

  • Western blot - Anti-Ras antibody [EP1125Y] (ab52939), expandable thumbnail

    Western blot - Anti-Ras antibody [EP1125Y] (ab52939)

    All lanes: Western blot - Anti-Ras antibody [EP1125Y] (ab52939) at 1/500000 dilution

    All lanes: C6 (rat glial tumor cell line) cell lysate at 10 µg/mL

    Secondary

    All lanes: goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 21 kDa

    Observed band size: 18 kDa

  • Flow Cytometry (Intracellular) - Anti-Ras antibody [EP1125Y] (ab52939), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ras antibody [EP1125Y] (ab52939)

    Unpurified ab52939 at 1/100 dilution staining Ras in permeabilized PC-12 (rat adrenal gland pheochromocytoma cell line) cells by intracellular flow cytometry (red). Rabbit IgG negative control (green).

  • Western blot - Anti-Ras antibody [EP1125Y] (ab52939), expandable thumbnail

    Western blot - Anti-Ras antibody [EP1125Y] (ab52939)

    All lanes: Western blot - Anti-Ras antibody [EP1125Y] (ab52939) at 1/500 dilution

    All lanes: Human Ras full length protein (ab56526) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa

    Exposure time: 1min

  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] (ab52939), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] (ab52939)

    ab52939 staining of GTPase KRas in a HCT 116 cell spheroid. The cells were fixed with 4% paraformaldehyde (10 min), permeabilised with 2% Triton X-100 for 1h and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween overnight at room temperature. The spheroids were then incubated overnight at room temperature with ab52939 at 2 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 2 μg/ml. DAPI was used as nuclear counterstain (shown in blue). As secondary antibodies Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat anti-Rabbit (Alexa Fluor® 488) (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat anti-Mouse (Alexa Fluor® 594) (shown in magenta) were used, incubated overnight at room temperature. All permeabilization, blocking and antibody incubation steps were performed using a rotary shaker.

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    Similar results were observed using 100% Methanol (5min).

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