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Anti-Ras antibody [EPR3255] ab108602 is a rabbit monoclonal antibody that is used in Ras western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your Ras staining


Images

Immunoprecipitation - Anti-Ras antibody [EPR3255] (AB108602), expandable thumbnail
  • Western blot - Anti-Ras antibody [EPR3255] (AB108602), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Ras antibody [EPR3255] (AB108602), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] (AB108602), expandable thumbnail
  • Western blot - Anti-Ras antibody [EPR3255] (AB108602), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Expected
Rat
Tested
Expected
Tested
Expected
Expected

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).

Alternative names

HRAS1, HRAS, GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras

Recommended products

Anti-Ras antibody [EPR3255] ab108602 is a rabbit monoclonal antibody that is used in Ras western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your Ras staining

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3255
Purification technique
Affinity purification Protein A
Specificity

ab108602 also detects H-Ras and N-Ras.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.

Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Associated diseases and disorders

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-Ras antibody [EPR3255] (ab108602), expandable thumbnail

    Immunoprecipitation - Anti-Ras antibody [EPR3255] (ab108602)

    ab108602 (purified) at 1/20 dilution (2 µg) immunoprecipitating Ras in SH-SY5Y whole cell lysate.
    Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab108602 & SH-SY5Y whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab108602 in SH-SY5Y whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Ras antibody [EPR3255] (ab108602)

    Predicted band size: 21 kDa

  • Western blot - Anti-Ras antibody [EPR3255] (ab108602), expandable thumbnail

    Western blot - Anti-Ras antibody [EPR3255] (ab108602)

    All lanes: Western blot - Anti-Ras antibody [EPR3255] (ab108602) at 1/10000 dilution

    Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

    Lane 2: Rat brain lysates at 20 µg

    Lane 3: Mouse heart lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Flow Cytometry (Intracellular) - Anti-Ras antibody [EPR3255] (ab108602), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ras antibody [EPR3255] (ab108602)

    Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Ras with purified ab108602 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] (ab108602), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] (ab108602)

    Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Ras with purified ab108602 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Ras antibody [EPR3255] (ab108602), expandable thumbnail

    Western blot - Anti-Ras antibody [EPR3255] (ab108602)

    All lanes: Western blot - Anti-Ras antibody [EPR3255] (ab108602) at 1/1000 dilution

    Lane 1: HEK-293 whole cell lysate at 10 µg

    Lane 2: SH-SY5Y cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labelling Ras with Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974 at 1/10000 (0.104 μg/ml) dilution followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution. Positive staining on rat cardiac muscle. The section was incubated with Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling Ras with Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974 at 1/10000 (0.104 μg/ml) dilution followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution. Positive staining on human breast carcinoma. The section was incubated with Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling Ras with Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974 at 1/10000 (0.104 μg/ml) dilution followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution. Positive staining on mouse cerebrum. The section was incubated with Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling Ras with Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974 at 1/10000 (0.104 μg/ml) dilution followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution. Positive staining on rat cerebrum. The section was incubated with Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Ras antibody [EPR3255] - BSA and Azide free ab209974).

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Product protocols

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