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AB221163

Anti-Ras (mutated G12D) antibody

2

(4 Reviews)

|

(19 Publications )

Rabbit Polyclonal RASK mutated G12D antibody. Suitable for WB and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human KRAS G12D.

View Alternative Names

KRAS2, RASK2, KRAS, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human KRAS G12D. The exact immunogen used to generate this antibody is proprietary information.

P01116

Specificity

<p>G12D mutant specific.</p>

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.
Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival (PubMed : 22711838, PubMed : 23698361). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed : 20949621, PubMed : 39809765). Activates MAPK1/MAPK3 resulting in phosphorylation and ultimately degradation of GJA1 (By similarity). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed : 24623306). Recognized by LZTR1 that mediates its ubiquitination by a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex (PubMed : 40934300).
See full target information KRAS G12D
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutical biology 63:343-356 PubMed40314193

2025

Delivery of small interfering RNA through lyophilized natural lipid nanoparticles: effects of natural lipid selection.

Applications

Unspecified application

Species

Unspecified reactive species

Hangjie Wang,Wei Li,Junyan Chen,Rong Chen,Yuwei Qi,Linshuang Shen,Kaidi Chen,Lewei Dai,Yuxin Sheng,An Wang,Hong Wang,Chujian Chen,Xiao Cheng,Mancang Gu

Molecular therapy : the journal of the American Society of Gene Therapy 33:1134-1153 PubMed39810420

2025

Pancreatic cancer-derived extracellular vesicles enhance chemoresistance by delivering KRAS protein to cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyuan Liu,Jiaqi Yang,Sicong Huang,Yifan Hong,Yupeng Zhu,Jianing Wang,Yi Wang,Tingbo Liang,Xueli Bai

JCI insight 9: PubMed39576014

2024

CRISPR/CasRx suppresses KRAS-induced brain arteriovenous malformation developed in postnatal brain endothelial cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shoji Saito,Yuka Nakamura,Satoshi Miyashita,Tokiharu Sato,Kana Hoshina,Masayasu Okada,Hitoshi Hasegawa,Makoto Oishi,Yukihiko Fujii,Jakob Körbelin,Yoshiaki Kubota,Kazuki Tainaka,Manabu Natsumeda,Masaki Ueno

Science advances 10:eadp0684 PubMed39413197

2024

Semaphorin 3D promotes pancreatic ductal adenocarcinoma progression and metastasis through macrophage reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Noelle R J Thielman,Vanessa Funes,Sanjana Davuluri,Hector E Ibanez,Wei-Chih Sun,Juan Fu,Keyu Li,Stephen Muth,Xingyi Pan,Kenji Fujiwara, Dwayne L Thomas Ii,MacKenzie Henderson,Selina Shiqing Teh,Qingfeng Zhu,Elizabeth Thompson,Elizabeth M Jaffee,Alex Kolodkin,Fengxi Meng,Lei Zheng

Pathology international 74:538-545 PubMed38994806

2024

Autopsy case of linear nevus sebaceous syndrome with KRAS (G12D) mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Akira Ohishi,Yasunori Enomoto,Hideto Iwafuchi,Shiori Meguro,Isao Kosugi,Satoshi Baba,Toshihide Iwashita,Yuki Segawa,Daizo Ueno,Shigeo Iijima

Molecular pharmaceutics 21:2034-2042 PubMed38456403

2024

KRAS Mutation Detection with (2,4)-4-[F]FGln for Noninvasive PDAC Diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Song Liu,Futao Liu,Xingguo Hou,Qian Zhang,Ya'nan Ren,Hua Zhu,Zhi Yang,Xiaoxia Xu

Molecular carcinogenesis 62:1284-1294 PubMed37222390

2023

KRAS mutations upregulate Runx1 to promote occurrence of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Deng,Jiaxin Guo,Zihang Ling,Chi Zhang,Lihong He,Zhaona Fan,Bin Cheng,Juan Xia

Cell death and differentiation 30:560-575 PubMed36539510

2022

Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hanshen Yang,Xiaozhen Zhang,Mengyi Lao,Kang Sun,Lihong He,Jian Xu,Yi Duan,Yan Chen,Honggang Ying,Muchun Li,Chengxiang Guo,Qingsong Lu,Sicheng Wang,Wei Su,Tingbo Liang,Xueli Bai

Molecular therapy. Nucleic acids 28:342-352 PubMed35474735

2022

Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method.

Applications

Unspecified application

Species

Unspecified reactive species

Osamu Shibata,Kenya Kamimura,Yuto Tanaka,Kohei Ogawa,Takashi Owaki,Chiyumi Oda,Shinichi Morita,Atsushi Kimura,Hiroyuki Abe,Satoshi Ikarashi,Kazunao Hayashi,Takeshi Yokoo,Shuji Terai

Experimental & molecular medicine 53:1559-1568 PubMed34635780

2021

Leukotriene B4 receptor-2 contributes to KRAS-driven lung tumor formation by promoting interleukin-6-mediated inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Hyun Jang,Donghwan Park,Guen-Soo Park,Dong-Wook Kwak,JaeIn Park,Dae-Yeul Yu,Hye Jin You,Jae-Hong Kim
View all publications
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