AB264094

Anti-Ras (mutated G12V) antibody

Name: Anti-Ras (mutated G12V) antibody (ab264094) | Abcam
Brand: Abcam Limited
SKU: AB264094
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal RASH mutated G12V antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HRAS mutated G12V.

View Alternative Names

HRAS1, HRAS, GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Ras (mutated G12V) antibody (AB264094)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ras (mutated G12V) antibody (AB264094)

Immunofluorescence analysis of RAS (WT) and RAS (G12V mutant) transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min labelling RAS (G12V Mutant) protein using ab264094 at a 1/500 dilution (Green).
DAPI was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-Ras (mutated G12V) antibody (AB264094)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Ras (mutated G12V) antibody (ab264094) at 1/3000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

RAS (WT) transfected (+) 293T whole cell extracts at 30 µg

Lane 3:

RAS (mutant G12V) transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human HRAS mutated G12V. The exact immunogen used to generate this antibody is proprietary information.

P01112

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.

Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the activation of Ras protein signal transduction (PubMed : 22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed : 12740440, PubMed : 14500341, PubMed : 9020151).

See full target information HRAS mutated G12V

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 200:61-80 PubMed23277425

2013

Neurobeachin regulates neurotransmitter receptor trafficking to synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Ramya Nair,Juliane Lauks,SangYong Jung,Nancy E Cooke,Heidi de Wit,Nils Brose,Manfred W Kilimann,Matthijs Verhage,JeongSeop Rhee

View all publications

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