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AB313698

Anti-RAS (mutated G12V) antibody [EPR26090-62]

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Rabbit Recombinant Monoclonal RASK mutated G12V antibody. Suitable for WB and reacts with Human, Transfected cell lysate - Human samples.

View Alternative Names

KRAS2, RASK2, KRAS, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras

4 Images
Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (AB313698)
  • WB

Supplier Data

Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (AB313698)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Positive control : SW620, SW480, CAPAN-1 (KRAS G12V) Negative control : THP-1 (N-RAS G12D), HCT 116 (K-RAS G13D), Calu-1 (K-RAS G12C), A549 (K-RAS G12S), Caco-2 (K-RAS wild-type) and HT-29 (K-RAS wild-type). This antibody specifically recognizes RAS (mutated G12V). In Western blot, anti-Ras antibody (ab275875) staining at 1/1000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (ab313698) at 1/1000 dilution

Lane 1:

SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 2:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 3:

Capan-1 (human pancreatic adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 4:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 48 µg

Lane 5:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 48 µg

Lane 6:

Calu-1 (human lu epidermoid carcinoma epithelial cell) whole cell lysate 48 at 48 µg

Lane 7:

A549 (human lung carcinoma epithelial cell) whole cell lysate 48 at 48 µg

Lane 8:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 9:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 21 kDa

false

Exposure time: 180s

Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (AB313698)
  • WB

Supplier Data

Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (AB313698)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Positive control : SW620, SW480, (KRAS G12V) Negative control : SW1116 (KRAS G12A), RPMI-8226 (KRAS G12A), MIA PaCa-2 (KRAS G12C), LoVo (KRAS G13D). This antibody specifically recognizes RAS (mutated G12V). In Western blot, anti-Ras antibody (ab275875) staining at 1/1000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (ab313698) at 1/1000 dilution

Lane 1:

SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 2:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 3:

SW1116 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 4:

RPMI-8226 (human plasmacytoma B Lymphocyte) whole cell lysate at 48 µg

Lane 5:

MIA PaCa-2 (human pancreas epithelial cell) whole cell lysate at 48 µg

Lane 6:

LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 21 kDa

false

Exposure time: 180s

Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (AB313698)
  • WB

Supplier Data

Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (AB313698)

Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody specifically recognizes RAS (mutated G12V). In Western blot, anti-Ras antibody (ab275875) staining at 1/1000 dilution. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (ab313698) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human KRAS (mutated G12V) expression vector containing a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human HRAS (mutated G12V) expression vector containing a His-tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human NRAS (mutated G12V) expression vector containing a His-tag whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a human KRAS expression vector containing a His-tag whole cell lysate at 40 µg

Lane 6:

293T cells transfected with a human KRAS (mutated G12C) expression vector containing a His-tag whole cell lysate at 40 µg

Lane 7:

293T cells transfected with a human KRAS (mutated G12A) expression vector containing a His-tag whole cell lysate at 20 µg

Lane 8:

293T cells transfected with a human KRAS (mutated G12D) expression vector containing a His-tag whole cell lysate at 20 µg

Lane 9:

293T cells transfected with a human KRAS (mutated G12S) expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 21 kDa

false

Exposure time: 180s

Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (AB313698)
  • WB

Supplier Data

Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (AB313698)

Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody specifically recognizes RAS (mutated G12V). In Western blot, anti-Ras antibody (ab275875) staining at 1/1000 dilution. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-RAS (mutated G12V) antibody [EPR26090-62] (ab313698) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human KRAS (mutated G12V) expression vector containing a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human KRAS (mutated G12R) expression vector containing a His-tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human KRAS (mutated G13D) expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 21 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-RAS (mutated G12V) antibody [EPR26090-62] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26090-62

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB264095

Anti-Ras (mutated G12V) antibody

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras (mutated Q61R) antibody [SP174] (AB227658)

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Secondary Antibodies

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  • 4
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.
Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed : 20949621, PubMed : 39809765). Plays an important role in the regulation of cell proliferation (PubMed : 22711838, PubMed : 23698361). Activates MAPK1/MAPK3 resulting in phosphorylation and ultimately degradation of GJA1 (By similarity). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed : 24623306).
See full target information KRAS mutated G12V
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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