AB289374

Anti-Ras (mutated G12V) antibody [HL169] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal RASK mutated G12V antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate, Human samples. Immunogen corresponding to Synthetic Peptide within Human KRAS mutated G12V.

View Alternative Names

KRAS2, RASK2, KRAS, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras

3 Images

Supplier Data

Western blot - Anti-Ras (mutated G12V) antibody [HL169] - BSA and Azide free (AB289374)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Ras (mutated G12V) antibody [HL169] - BSA and Azide free (ab289374) at 1/1000 dilution

Lane 1:

MDA-MB-468 (KRAS WT) whole cell extracts at 30 µg

Lane 2:

CFPAC-1 (KRAS G12V) whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Ras (mutated G12V) antibody [HL169] - BSA and Azide free (AB289374)

12% SDS-PAGE

All lanes:

Western blot - Anti-Ras (mutated G12V) antibody [HL169] - BSA and Azide free (ab289374) at 1/1000 dilution

Lane 1:

HEK293T wild type whole cell extract at 30 µg

Lane 2:

HEK293T Myc-DDK-tag tagged Ras/wild type whole cell extract at 30 µg

Lane 3:

HEK293T DDDDK-tag tagged Ras (mutant G12D) whole cell extract at 30 µg

Lane 4:

HEK293T HA-tag tagged Ras (mutant G12V) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Ras (mutated G12V) antibody [HL169] - BSA and Azide free (AB289374)

12% SDS-PAGE

All lanes:

Western blot - Anti-Ras (mutated G12V) antibody [HL169] - BSA and Azide free (ab289374) at 1/1000 dilution

Lane 1:

HDPE (KRAS WT) whole cell extract at 30 µg

Lane 2:

AsPC1 (KRAS G12D) whole cell extract at 30 µg

Lane 3:

PANC-1 (KRAS G12D) whole cell extract at 30 µg

Lane 4:

CFPA-1 (KRAS G12V) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL169

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human KRAS mutated G12V. The exact immunogen used to generate this antibody is proprietary information.

P01116

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.

Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed : 20949621, PubMed : 39809765). Plays an important role in the regulation of cell proliferation (PubMed : 22711838, PubMed : 23698361). Activates MAPK1/MAPK3 resulting in phosphorylation and ultimately degradation of GJA1 (By similarity). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed : 24623306).

See full target information KRAS mutated G12V

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ras (mutated G12V) antibody [HL169] - BSA and Azide free

Western blot - Anti-Ras (mutated G12V) antibody [HL169] - BSA and Azide free (AB289374)

Western blot - Anti-Ras (mutated G12V) antibody [HL169] - BSA and Azide free (AB289374)

Western blot - Anti-Ras (mutated G12V) antibody [HL169] - BSA and Azide free (AB289374)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise