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AB242415

Anti-Ras (mutated Q61) antibody [EPR20278] - BSA and Azide free

4

(3 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal RASN mutated Q61R antibody. Carrier free. Suitable for WB and reacts with Transfected cell line - Human, Human samples. Cited in 2 publications.

View Alternative Names

HRAS1, NRAS, GTPase NRas, Transforming protein N-Ras

2 Images
Western blot - Anti-Ras (mutated Q61) antibody [EPR20278] - BSA and Azide free (AB242415)
  • WB

Supplier Data

Western blot - Anti-Ras (mutated Q61) antibody [EPR20278] - BSA and Azide free (AB242415)

This data was developed using ab222516, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

SK-MEL-2 cell line contains N-Ras Q61R homozygous mutation. HT-1197 cell line contains N-Ras Q61R heterozygous mutation. MeWo cell line is wild-type for N-Ras.

All lanes:

Western blot - Anti-Ras (mutated Q61) antibody [EPR20278] (<a href='/en-us/products/primary-antibodies/ras-mutated-q61-antibody-epr20278-ab222516'>ab222516</a>) at 1/1000 dilution

Lane 1:

SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate at 10 µg

Lane 2:

HT-1197 (human urinary bladder carcinoma cell line) whole cell lysate at 10 µg

Lane 3:

MeWo (human malignant melanoma fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 20 kDa

true

Exposure time: 30s

Western blot - Anti-Ras (mutated Q61) antibody [EPR20278] - BSA and Azide free (AB242415)
  • WB

Lab

Western blot - Anti-Ras (mutated Q61) antibody [EPR20278] - BSA and Azide free (AB242415)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM /TBST This antibody has been found to react with different mutations we tested. We haven’t tested all possible mutations at Q61. This data was developed using ab222516, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Ras (mutated Q61) antibody [EPR20278] (<a href='/en-us/products/primary-antibodies/ras-mutated-q61-antibody-epr20278-ab222516'>ab222516</a>) at 1/1000 dilution

Lane 1:

HEK-293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a His-tag at 20 µg

Lane 2:

HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged NRAS (mutated Q61R) whole cell lysate at 20 µg

Lane 3:

HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged NRAS (mutated Q61K) whole cell lysate at 20 µg

Lane 4:

HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged NRAS (mutated Q61H) whole cell lysate at 20 µg

Lane 5:

HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged NRAS (mutated Q61L) whole cell lysate at 20 µg

Lane 6:

HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged NRAS whole cell lysate at 20 µg

Lane 7:

HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged HRAS (mutated Q61R) whole cell lysate at 20 µg

Lane 8:

HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged KRAS (mutated Q61R) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 25 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20278

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 95% homology with NRAS, KRAS and HRAS.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Transfected cell line - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab242415 is the carrier-free version of ab222516.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.
Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
See full target information NRAS Q61R
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101572 PubMed38754420

2024

CD37 is a safe chimeric antigen receptor target to treat acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Caulier,Sandy Joaquina,Pascal Gelebart,Tara Helén Dowling,Fatemeh Kaveh,Moritz Thomas,Luka Tandaric,Patrik Wernhoff,Niveditha Umesh Katyayini,Cara Wogsland,May Eriksen Gjerstad,Yngvar Fløisand,Gunnar Kvalheim,Carsten Marr,Sebastian Kobold,Jorrit M Enserink,Bjørn Tore Gjertsen,Emmet McCormack,Else Marit Inderberg,Sébastien Wälchli

Neoplasma 69:383-391 PubMed35225647

2022

The Hu antigen R/interferon-β axis modulates sensitivity of esophageal squamous cancer cells to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Yun Cui,Xiao-Feng Zhu,Shu Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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