Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free (ab243933)

Rabbit Recombinant Monoclonal RASN mutated Q61R antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Transfected cell line - Human, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free (AB243933), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	Flow Cyt (Intra)
Human	Tested	Tested	Tested
Transfected cell line - Human	Tested	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Human, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info -	Notes Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. This antibody may not be able to detect endogenous NRAS Q61K in patient tissue samples by IHC-P based on a collaborator's data.
Species Human	Dilution info -	Notes Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. This antibody may not be able to detect endogenous NRAS Q61K in patient tissue samples by IHC-P based on a collaborator's data.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info -	Notes -

Associated Products

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Target data

See full target information NRAS mutated Q61R

Function

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Alternative names

HRAS1, NRAS, GTPase NRas, Transforming protein N-Ras

Recommended products

Rabbit Recombinant Monoclonal RASN mutated Q61R antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Transfected cell line - Human, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: SP174
Purification technique: Affinity purification Protein A/G
Specificity: This antibody has been found to react with different mutations we tested. We have not tested all possible mutations at Q61.
Concentration
Purification notes: Purified from TCS by protein A/G.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab243933 is the carrier-free version of Anti-Ras (mutated Q61R) antibody [SP174] ab227658.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.

Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Associated diseases and disorders

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

This image is courtesy of an anonymous collaborator.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free (ab243933)
Immunohistochemical analysis of paraffin-embedded human primary malignant melanoma tissue (mutation status: NRAS Q61R) labeling NRAS with Anti-Ras (mutated Q61R) antibody [SP174] ab227658 at 1/100 dilution for 30 min at RT. The tissue was counterstained with Hematoxylin. Heat mediated antigen retrieval was achieved by using EDTA buffer pH 9.0 before IHC staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Ras (mutated Q61R) antibody [SP174] ab227658)
This image is courtesy of an anonymous collaborator.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free (ab243933)
Immunohistochemical analysis of paraffin-embedded human primary malignant melanoma tissue (mutation status: NRAS Q61R) labeling NRAS with Anti-Ras (mutated Q61R) antibody [SP174] ab227658 at 1/100 dilution for 30 min at RT. The tissue was counterstained with Hematoxylin. Heat mediated antigen retrieval was achieved by using EDTA buffer pH 9.0 before IHC staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Ras (mutated Q61R) antibody [SP174] ab227658)
This image is courtesy of an anonymous collaborator.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free (ab243933)
Immunohistochemical analysis of paraffin-embedded human primary malignant melanoma tissue (mutation status: NRAS Q61R) labeling NRAS with Anti-Ras (mutated Q61R) antibody [SP174] ab227658 at 1/100 dilution for 30 min at RT. The tissue was counterstained with Hematoxylin. Heat mediated antigen retrieval was achieved by using EDTA buffer pH 9.0 before IHC staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Ras (mutated Q61R) antibody [SP174] ab227658)
This image is courtesy of an anonymous collaborator.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free (ab243933)
Immunohistochemical analysis of paraffin-embedded human metastatic malignant melanoma tissue (mutation status: NRAS Q61R) labeling NRAS with Anti-Ras (mutated Q61R) antibody [SP174] ab227658 at 1/100 dilution for 30 min at RT. The tissue was counterstained with Hematoxylin. Heat mediated antigen retrieval was achieved by using EDTA buffer pH 9.0 before IHC staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Ras (mutated Q61R) antibody [SP174] ab227658)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free (ab243933)
Immunohistochemical analysis of paraffin-embedded human metastatic malignant melanoma tissue (mutation status: NRAS Q61K) labeling NRAS with Anti-Ras (mutated Q61R) antibody [SP174] ab227658 at 1/100 dilution for 30 min at RT. The tissue was counterstained with Hematoxylin. Heat mediated antigen retrieval was achieved by using EDTA buffer pH 9.0 before IHC staining.
Immunohistochemistry - Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free (ab243933)
Formalin-fixed, paraffin-embedded human SK-MEL-2 cells stained for NRAS (mutated Q61R) using Anti-Ras (mutated Q61R) antibody [SP174] ab227658 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Ras (mutated Q61R) antibody [SP174] ab227658).
Flow Cytometry (Intracellular) - Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free (ab243933)
Intracellular Flow Cytometry analysis of SK-MEL-2 (Human skin malignant melanoma) cells labeling NRAS with purifiedab227658 at 1/200 dilution (0.8μg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor© 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ras (mutated Q61R) antibody [SP174] ab227658).
Western blot - Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free (ab243933)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBST
This antibody has been found to react with different mutations we tested. We haven’t tested all possible mutations at Q61.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Ras (mutated Q61R) antibody [SP174] ab227658).
All lanes: Western blot - Anti-Ras (mutated Q61R) antibody [SP174] (Anti-Ras (mutated Q61R) antibody [SP174] ab227658) at 1/1000 dilution
Lane 1: HEK-293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a His-tag, whole cell lysate at 20 µg
Lane 2: HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged NRAS (mutated Q61R) whole cell lysate at 20 µg
Lane 3: HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged NRAS (mutated Q61K) whole cell lysate at 20 µg
Lane 4: HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged NRAS (mutated Q61H) whole cell lysate at 20 µg
Lane 5: HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged NRAS (mutated Q61L) whole cell lysate at 20 µg
Lane 6: HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged NRAS whole cell lysate at 20 µg
Lane 7: HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged HRAS (mutated Q61R) whole cell lysate at 20 µg
Lane 8: HEK-293T (Human embryonic kidney epithelial cell) transfected with His-tagged KRAS (mutated Q61R) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa
Exposure time: 5s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free (ab243933)
Immunohistochemistry analysis of paraffin-embedded (A) HEK-293T transfected with NRAS (Q61R) expression vector, (B) HEK-293T transfected with NRAS (Q61K) expression vector, (C) HEK-293T transfected with NRAS (Q61H) expression vector, (D) HEK-293T transfected with NRAS (Q61L) expression vector, (E) HEK-293T transfected with NRAS expression vector, (F) HEK-293T transfected with HRAS (Q61R) expression vector, (G) HEK-293T transfected with KRAS(Q61R) and (H) HEK-293T transfected empty vector, sections labelling NRAS (mutated Q61R) with Anti-Ras (mutated Q61R) antibody [SP174] ab227658 at 1/100 dilution. The section was incubated with Anti-Ras (mutated Q61R) antibody [SP174] ab227658 for 10 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on HEK-293T transfected with NRAS (Q61R) expression vector (Image A), HEK-293T transfected with NRAS (Q61K) expression vector (Image B), HEK-293T transfected with HRAS (Q61R) expression vector (Image F) and HEK-293T transfected with KRAS (Q61R) expression vector (Image G); Nearly no staining on HEK-293T transfected with NRAS (Q61H) expression vector (Image C), HEK-293T transfected with NRAS (Q61L) expression vector (Image D), HEK-293T transfected with NRAS expression vector (Image E) and HEK-293T transfected empty vector (Image H). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Ras (mutated Q61R) antibody [SP174] ab227658).