Anti-RASA1 antibody [B4F8]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (AB2922)

ab2922 labelling GAP (green) in the cytoplasm of Hela cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (AB2922)

ab2922 labelling GAP (green) in the cytoplasm of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• WB

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Western blot - Anti-RASA1 antibody [B4F8] (AB2922)

All lanes:

Western blot - Anti-RASA1 antibody [B4F8] (ab2922) at 1/200 dilution

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

A431 cell lysate at 25 µg

Lane 3:

Mouse brain cell lysate at 25 µg

Predicted band size: 116 kDa

Observed band size: 110 kDa

false

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (AB2922)

ab2922 labelling GAP (green) in the cytoplasm of A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.