AB40677

Anti-RASA1 antibody [EP536Y]

20ul selling size
RabMAb
Recombinant
What is this?

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(12 Publications)

Rabbit Recombinant Monoclonal RASA1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 12 publications.

View Alternative Names

GAP, RASA, RASA1, Ras GTPase-activating protein 1, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA1 antibody [EP536Y] (AB40677)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA1 antibody [EP536Y] (AB40677)

Immunohistochemical analysis of paraffin embedded human ovary carcinoma using ab40677 diluted 1 : 50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-RASA1 antibody [EP536Y] (AB40677)

All lanes:

Western blot - Anti-RASA1 antibody [EP536Y] (ab40677) at 1/2000 dilution

All lanes:

Rat brain tissue lysate at 10 µg

Predicted band size: 116 kDa

Observed band size: 140 kDa,55 kDa

false

Carrier free

Anti-RASA1 antibody [EP536Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP536Y

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase-activating protein (GAP) that stimulates the intrinsic GTPase activity of Ras proteins, such as NRAS, facilitating their transition from the active GTP-bound state to the inactive GDP-bound state, thereby terminating Ras signaling.

See full target information RASA1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e13204 PubMed36747515

2023

MEG3 sponges miRNA-376a and YBX1 to regulate angiogenesis in ovarian cancer endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yize Li,Lingling Zhang,Yongmei Zhao,Hongyan Peng,Nan Zhang,Wendong Bai

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 30:282-291 PubMed34897876

2021

Identification of the circ_PRKDC/miR-20a-3p/RASA1 axis in regulating HaCaT keratinocyte migration.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Na Jiang,Xiaohui Ji,Wei Liu,Chuanchuan Qi,Xiaomei Zhai

Molecular medicine (Cambridge, Mass.) 27:73 PubMed34238206

2021

CircMTO1 inhibits ox-LDL-stimulated vascular smooth muscle cell proliferation and migration via regulating the miR-182-5p/RASA1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ningning Ji,Yu Wang,Xinyan Gong,Shimao Ni,Hui Zhang

Molecular therapy. Nucleic acids 24:164-174 PubMed33767913

2021

Exosome-transmitted miRNA-335-5p promotes colorectal cancer invasion and metastasis by facilitating EMT via targeting RASA1.

Applications

Unspecified application

Species

Unspecified reactive species

Xuecheng Sun,Feiyan Lin,Wenjing Sun,Weijian Zhu,Daoquan Fang,Lifang Luo,Shuhan Li,Wenqi Zhang,Lei Jiang

Annals of translational medicine 9:104 PubMed33569406

2021

RNA-binding protein QKI suppresses breast cancer via RASA1/MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Cao,Chengyu Chu,Xiaoyan Li,Siwen Gu,Qiang Zou,Yiting Jin

Oncology letters 18:6361-6370 PubMed31814846

2019

Colorectal cancer cells differentially impact migration and microRNA expression in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Do Yei Kim,Sang-Soo Lee,Young-Kyung Bae

Molecular medicine reports 19:3393-3402 PubMed30864691

2019

MicroRNA‑4530 suppresses cell proliferation and induces apoptosis by targeting RASA1 in human umbilical vein endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Jing,Hong Li,Tao Zhang,Jianxin Lu,Lianjin Zhong

Oncology letters 14:5386-5392 PubMed29098030

2017

Co-expression and significance of Dok2 and Ras p21 protein activator 1 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangrong Huang,Xiaochun Peng,Kun Zhang,Chunyan Li,Bo Su,Yanxiang Zhang,Wangui Yu

Anticancer research 37:781-785 PubMed28179330

2017

Down-regulation of RASA1 Is Associated with Poor Prognosis in Human Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Li Chen,Wei-Chieh Huang,Hsin-Lei Yao,Po-Ming Chen,Ping-Yi Lin,Fu-Yu Feng,Pei-Yi Chu

Scientific reports 6:35308 PubMed27752061

2016

Protein tyrosine phosphatase 1B targets PITX1/p120RasGAP thus showing therapeutic potential in colorectal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Wei Teng,Man-Hsin Hung,Li-Ju Chen,Mao-Ju Chang,Feng-Shu Hsieh,Ming-Hsien Tsai,Jui-Wen Huang,Chih-Lung Lin,Hsiang-Wen Tseng,Zong-Keng Kuo,Jeng-Kai Jiang,Shung-Haur Yang,Chung-Wai Shiau,Kuen-Feng Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RASA1 antibody [EP536Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA1 antibody [EP536Y] (AB40677)

Western blot - Anti-RASA1 antibody [EP536Y] (AB40677)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (12)

Product promise