JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB247875

Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal RASA1 phospho Y460 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

GAP, RASA, RASA1, Ras GTPase-activating protein 1, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] - BSA and Azide free (AB247875)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] - BSA and Azide free (AB247875)

This data was developed using ab109465, the same antibody clone in a different buffer formulation.ab109465 at 1/100 dilution staining GAP in 293T cells by Immunofluorescence.

Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] - BSA and Azide free (AB247875)
  • WB

Unknown

Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] - BSA and Azide free (AB247875)

This data was developed using ab109465, the same antibody clone in a different buffer formulation.

Note : The lower panel utilized an alternative anti-GAP antibody which does not require phosphorylation for detection of the antigen.

All lanes:

Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (<a href='/en-us/products/primary-antibodies/rasa1-phospho-y460-antibody-epr26202-ab109465'>ab109465</a>) at 1/1000 dilution

Lane 1:

293T cell lysates, untreated at 10 µg

Lane 2:

293T cell lysates treated with Pervanadate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 116 kDa

false

  • Unconjugated

    Anti-RASA1 (phospho Y460) antibody [EPR2620(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2620(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects GAP phosphorylated at Tyrosine 460&#46;

style
left-column

Complementary Products

Primary Antibodies

AB52939

Anti-Ras antibody [EP1125Y]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] (AB52939)

  • 5
  • 12
Primary Antibodies

AB247442

Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)

  • 0
  • 0
Primary Antibodies

AB302940

Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [10-PLC-gamma]

Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [10-PLC-gamma] (AB302940)

  • 0
  • 0
Primary Antibodies

AB109541

Anti-VASP (phospho S156) antibody [EPR1065(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S156) antibody [EPR1065(2)] (AB109541)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab247875 is the carrier-free version of ab109465.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

GTPase-activating protein (GAP) that stimulates the intrinsic GTPase activity of Ras proteins, such as NRAS, facilitating their transition from the active GTP-bound state to the inactive GDP-bound state, thereby terminating Ras signaling.
See full target information RASA1 pY460
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com