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AB323554

Anti-RASA2 antibody [EPR29503-39]

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Rabbit Recombinant Monoclonal RASA2 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples.

View Alternative Names

GAP1M, RASGAP, RASA2, Ras GTPase-activating protein 2, GTPase-activating protein 1m, GAP1m

4 Images
Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)
  • WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : skeletal muscle

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

Human thyroid tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 96 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)
  • WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : PC-3

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

DLD-1 (human colorectal adenocarcinoma epithelial cell) whole lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 96 kDa,124 kDa

false

Exposure time: 180s

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)
  • WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1-4 : 92 seconds, lane 5 : 114 seconds

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole lysate at 20 µg

Lane 2:

HFF-1 (human skin fibroblast) whole lysate at 20 µg

Lane 3:

A431 (human epidermoid carcinoma epithelial cell) whole lysate at 20 µg

Lane 4:

NCI-H1299 (human lung carcinoma epithelial cell) whole lysate at 20 µg

Lane 5:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

Observed band size: 96 kDa,36 kDa

false

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)
  • WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human RASA3 by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

His-tagged human RASA2 recombinant protein at 10 ng

Lane 2:

His-tagged human RASA3 recombinant protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 96 kDa,33 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29503-39

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4).
See full target information RASA2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com