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AB153846

Anti-RASA3 antibody

5

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal RASA3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Ras GTPase-activating protein 3 aa 550 to C-terminus.

View Alternative Names

Ras GTPase-activating protein 3, GAP1(IP4BP), Ins P4-binding protein, RASA3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA3 antibody (AB153846)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA3 antibody (AB153846)

Immunohistochemical analysis of paraffin-embedded Human MCF7 xenograft tissue labeling RASA3 with ab153846 at 1/100 dilution.

Western blot - Anti-RASA3 antibody (AB153846)
  • WB

Supplier Data

Western blot - Anti-RASA3 antibody (AB153846)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-RASA3 antibody (ab153846) at 1/500 dilution

Lane 1:

SK-N-SH whole cell extracts at 30 µg

Lane 2:

IMR32 whole cell extracts at 30 µg

Lane 3:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-RASA3 antibody (AB153846)
  • WB

AbReview46661****

Western blot - Anti-RASA3 antibody (AB153846)

Blocking agent : 3% BSA for 60 minutes at 20oC.

All lanes:

Western blot - Anti-RASA3 antibody (ab153846) at 1/400 dilution

Lane 1:

Mouse platelet whole cell lysate at 20 µg

Lane 2:

Human platelet whole cell lysate at 20 µg

Secondary

All lanes:

IRDye 800CW - conjugated Oddyssey goat IgG at 1/15000 dilution

Predicted band size: 96 kDa

Observed band size: 98 kDa

false

Exposure time: 1hr

This image is courtesy of an anonymous Abreview.

Western blot - Anti-RASA3 antibody (AB153846)
  • WB

Supplier Data

Western blot - Anti-RASA3 antibody (AB153846)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-RASA3 antibody (ab153846) at 1/1000 dilution

All lanes:

Mouse brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Ras GTPase-activating protein 3 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14644

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor.
See full target information Ras GTPase-activating protein 3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:104718 PubMed37062417

2023

The Sec1-Munc18 protein VPS33B forms a uniquely bidirectional complex with VPS16B.

Applications

Unspecified application

Species

Unspecified reactive species

Richard J Y Liu,Yusef Al-Molieh,Shao Z Chen,Marko Drobac,Denisa Urban,Chang H Chen,Helen H Y Yao,Ryan S Q Geng,Ling Li,Fred G Pluthero,Samir Benlekbir,John L Rubinstein,Walter H A Kahr

Science signaling 15:eabl9169 PubMed35857633

2022

A CRISPR screen targeting PI3K effectors identifies RASA3 as a negative regulator of LFA-1-mediated adhesion in T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kristoffer H Johansen,Dominic P Golec,Bonnie Huang,Chung Park,Julie H Thomsen,Silvia Preite,Jennifer L Cannons,Fabien Garçon,Edward C Schrom,Christina J F Courrèges,Tibor Z Veres,James Harrison,Meritxell Nus,James D Phelan,Wolfgang Bergmeier,John H Kehrl,Klaus Okkenhaug,Pamela L Schwartzberg
View all publications
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Product promise

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