Rabbit Recombinant Monoclonal RASA4 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (AB157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130695.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (AB157190)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (AB157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77176.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (AB157190)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (AB157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126008.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (AB157190)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (AB157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114228.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (AB157190)")
![Western blot - Anti-RASA4 antibody [EPR10515] (AB157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--western-blot-img88209.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RASA4 antibody [EPR10515] (AB157190)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Tested | Not recommended | Not recommended | Expected |
| Rat | Predicted | Not recommended | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Ca(2+)-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium. Functions as an adaptor for Cdc42 and Rac1 during FcR-mediated phagocytosis.
Alternative names
CAPRI, GAPL, KIAA0538, RASA4, Ras GTPase-activating protein 4, Calcium-promoted Ras inactivator, Ras p21 protein activator 4, RasGAP-activating-like protein 2
Recommended products
Rabbit Recombinant Monoclonal RASA4 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR10515
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130695.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
ab157190 showing +ve staining in Mouse kidney.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77176.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
ab157190 showing +ve staining in Human thyroid gland carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126008.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
ab157190 showing +ve staining in Human ovarian carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114228.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
ab157190 showing +ve staining in Human heart.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-RASA4 antibody [EPR10515] (ab157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--western-blot-img88209.jpg "Western blot - Anti-RASA4 antibody [EPR10515] (ab157190)")
Western blot - Anti-RASA4 antibody [EPR10515] (ab157190)
All lanes: Western blot - Anti-RASA4 antibody [EPR10515] (ab157190) at 1/1000 dilution
Lane 1: Human skeletal muscle lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 90 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128730.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling RASA4 with ab157190 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130959.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling RASA4 with ab157190 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128730.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190), expandable thumbnail](https://content.abcam.com/products/images/rasa4-antibody-epr10515-ab157190--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130959.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com