JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB121577

Anti-RASGRF2 antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal RASGRF2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASGRF2 aa 700-850.

View Alternative Names

GRF2, RASGRF2, Ras-specific guanine nucleotide-releasing factor 2, Ras-GRF2, Ras guanine nucleotide exchange factor 2

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-RASGRF2 antibody (AB121577)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RASGRF2 antibody (AB121577)

ab121577, at 4 μg/ml, staining RASGRF2 in PFA/Triton X-100 treated Human cell line A-431 by immunofluorescence. ab121577 staining is shown in green.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Immunohistochemical analysis of human testis tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in cells in the seminiferous ducts.

Western blot - Anti-RASGRF2 antibody (AB121577)
  • WB

Unknown

Western blot - Anti-RASGRF2 antibody (AB121577)

All lanes:

Western blot - Anti-RASGRF2 antibody (ab121577) at 1/250 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 141 kDa

true

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Immunohistochemical analysis of human cerebral cortex tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in neuronal cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Immunohistochemical analysis of human prostate tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Immunohistochemical analysis of human placenta tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in trophoblastic cells.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RASGRF2 aa 700-850. The exact immunogen used to generate this antibody is proprietary information.

O14827

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p>Use buffer at pH 6 for antigen retrieval.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.
See full target information RASGRF2
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 12:7177-7189 PubMed34729119

2021

High Expression of ERK-related RASGRF2 predicts Poor prognosis in patients with Stomach Adenocarcinoma and correlates with M2 macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqi Du,Zhengguang Wang,Weina Wan

Pigment cell & melanoma research 34:1074-1083 PubMed34310073

2021

RASGRF2 gene fusions identified in a variety of melanocytic lesions with distinct morphological features.

Applications

Unspecified application

Species

Unspecified reactive species

Aurélie Houlier,Daniel Pissaloux,Franck Tirode,Noémie Lopez Ramirez,Maud Plaschka,Julie Caramel,Ingrid Masse,Arnaud de la Fouchardiere

PloS one 15:e0231711 PubMed32298357

2020

Reciprocal expression of Annexin A6 and RasGRF2 discriminates rapidly growing from invasive triple negative breast cancer subsets.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Y Korolkova,Sarrah E Widatalla,Diva S Whalen,Gladys N Nangami,Adeniyi Abimbola,Stephen D Williams,Heather K Beasley,Emily Reisenbichler,Mary Kay Washington,Josiah Ochieng,Ingrid A Mayer,Brian D Lehmann,Amos M Sakwe

Cancer biology & therapy 20:435-443 PubMed30359168

2018

RasGRF2 promotes migration and invasion of colorectal cancer cells by modulating expression of MMP9 through Src/Akt/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peifen Lu,Junjun Chen,Lihong Yan,Lijun Yang,Litao Zhang,Jie Dai,Zixuan Hao,Tao Bai,Yanfeng Xi,Yahui Li,Zhiming Kang,Jun Xv,Gongqin Sun,Tao Yang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB226973

Anti-RASGRF2 antibody - C-terminal

Western blot - Anti-RASGRF2 antibody - C-terminal (AB226973)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB169027

Anti-PREX2 antibody

Western blot - Anti-PREX2 antibody (AB169027)

  • 0
  • 0
Primary Antibodies

AB121396

Anti-TBC1D15 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (AB121396)

  • 5
  • 1
Primary Antibodies

AB121743

Anti-C8orf55 antibody

Immunocytochemistry - Anti-C8orf55 antibody (AB121743)

  • 0
  • 0
Primary Antibodies

AB121927

Anti-UBE3D antibody

Immunocytochemistry - Anti-UBE3D antibody (AB121927)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com