Rabbit Polyclonal RASGRF2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASGRF2 aa 700-850.
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- Journal of Cancer 12:7177-71892021High Expression of ERK-related RASGRF2 predicts Poor prognosis in patients with Stomach Adenocarcinoma and correlates with M2 macrophage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaqi Du,Zhengguang Wang,Weina WanPubMed 34729119
- Pigment cell & melanoma research 34:1074-10832021RASGRF2 gene fusions identified in a variety of melanocytic lesions with distinct morphological features.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAurélie Houlier,Daniel Pissaloux,Franck Tirode,Noémie Lopez Ramirez,Maud Plaschka,Julie Caramel,Ingrid Masse,Arnaud de la FouchardierePubMed 34310073
- PloS one 15:e02317112020Reciprocal expression of Annexin A6 and RasGRF2 discriminates rapidly growing from invasive triple negative breast cancer subsets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlga Y Korolkova et. al.PubMed 32298357
- Cancer biology & therapy 20:435-4432018RasGRF2 promotes migration and invasion of colorectal cancer cells by modulating expression of MMP9 through Src/Akt/NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeifen Lu,Junjun Chen,Lihong Yan,Lijun Yang,Litao Zhang,Jie Dai,Zixuan Hao,Tao Bai,Yanfeng Xi,Yahui Li,Zhiming Kang,Jun Xv,Gongqin Sun,Tao YangPubMed 30359168
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RASGRF2 aa 700-850. The exact immunogen used to generate this antibody is proprietary information. Database link O14827
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Use buffer at pH 6 for antigen retrieval. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250.00000 - 1/500.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
Associated Products
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Target data
Function
Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.
Alternative names
GRF2, RASGRF2, Ras-specific guanine nucleotide-releasing factor 2, Ras-GRF2, Ras guanine nucleotide exchange factor 2
Recommended products
Rabbit Polyclonal RASGRF2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASGRF2 aa 700-850.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RASGRF2 aa 700-850. The exact immunogen used to generate this antibody is proprietary information. Database link O14827
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (ab121577)
Immunohistochemical analysis of human testis tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in cells in the seminiferous ducts.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (ab121577)
Immunohistochemical analysis of human prostate tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in glandular cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (ab121577)
Immunohistochemical analysis of human cerebral cortex tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in neuronal cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (ab121577)
Immunohistochemical analysis of human placenta tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in trophoblastic cells.
Western blot - Anti-RASGRF2 antibody (ab121577)
All lanes: Western blot - Anti-RASGRF2 antibody (ab121577) at 1/250 dilution
Lane 1: RT-4 cell lysate
Lane 2: U-251 MG cell lysate
Lane 3: Human plasma
Lane 4: Human liver lysate
Lane 5: Human tonsil lysate
Developed using the ECL technique.
Predicted band size: 141 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RASGRF2 antibody (ab121577)
ab121577, at 4 μg/ml, staining RASGRF2 in PFA/Triton X-100 treated Human cell line A-431 by immunofluorescence.
ab121577 staining is shown in green.
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