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AB317419

Anti-Rasgrp1 antibody [S02-2G4]

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Rabbit Monoclonal GRP1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human RASGRP1.

View Alternative Names

RASGRP, RAS guanyl-releasing protein 1, Calcium and DAG-regulated guanine nucleotide exchange factor II, Ras guanyl-releasing protein, CalDAG-GEFII

1 Images
Western blot - Anti-Rasgrp1 Antibody [S02-2G4] (AB317419)
  • WB

Supplier Data

Western blot - Anti-Rasgrp1 Antibody [S02-2G4] (AB317419)

All lanes:

Western blot - Anti-Rasgrp1 Antibody [S02-2G4] (ab317419) at 1/1000 dilution

Lane 1:

A549 cell lysate

Lane 2:

HL-60 cell lysate

Lane 3:

U251 cell lysate

Predicted band size: 90 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

S02-2G4

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human RASGRP1.

O95267

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Ras guanyl-releasing protein 1 (Rasgrp1) also known as CalDAG-GEFII or GRP1 acts as a guanine nucleotide exchange factor (GEF) for Ras and Rap1. These are small GTPases important to intracellular signaling. Rasgrp1 helps convert inactive GDP-bound Ras to its active GTP-bound form which triggers downstream signaling pathways. The protein has a molecular mass of approximately 93 kDa. Rasgrp1 is expressed in various tissues notably in the immune system compartments such as thymus and spleen.
Biological function summary

Rasgrp1 plays an important role in the activation and differentiation of immune cells. It participates in signaling pathways that regulate the function of T-cells and B-cells being critical in their development and response to antigens. Its role is not solitary but part of a larger signaling complex that coordinates the signaling processes initiated by the T-cell receptor. Such activities enable proper immune responses and control the immune system balance.

Pathways

Rasgrp1 integrates into the Ras-MAPK and Rap1 signaling pathways which have pivotal roles in cell growth differentiation and survival. Within these pathways it interacts with related proteins such as Sos (Son of Sevenless) and Raf kinases. The involvement of Rasgrp1 in the Ras-MAPK pathway exemplifies its importance in modulating cellular responses to external stimuli especially those related to immune system activation.

Rasgrp1 mutations or dysregulation associate with immune system disorders like systemic lupus erythematosus (SLE) and some forms of immunodeficiency. Alterations in Rasgrp1 expression can disrupt normal immune function leading to autoimmune conditions. Connections to these diseases often highlight interactions with proteins like Bcl10 and CARMA1 which also participate in the immune response regulation and signal transduction associated with these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP (PubMed : 15899849, PubMed : 23908768, PubMed : 27776107, PubMed : 29155103). Activates the Erk/MAP kinase cascade (PubMed : 15899849). Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras (PubMed : 10807788, PubMed : 12839994, PubMed : 27776107, PubMed : 29155103). Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways (PubMed : 19933860). Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses. May also function in differentiation of other cell types (PubMed : 12845332).
See full target information RASGRP1
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