AB124823

Anti-RASGRP3 (phospho T133) antibody [EPNCIR148]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Recombinant Monoclonal GRP3 phospho T133 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

GRP3, KIAA0846, RASGRP3, Ras guanyl-releasing protein 3, Calcium and DAG-regulated guanine nucleotide exchange factor III, Guanine nucleotide exchange factor for Rap1

1 Images

Unknown

Western blot - Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (AB124823)

All lanes:

Western blot - Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823) at 1/1000 dilution

Lane 1:

Ramos cell lysate, untreated at 10 µg

Lane 2:

Ramos cell lysate treated with Lambda Phosphatase at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 78 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR148

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Peter Blumberg. View antibodies from NCI Center for Cancer Research Collaboration.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Guanine nucleotide exchange factor (GEF) for Ras and Rap1.

See full target information RASGRP3 phospho T133

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 104:e14602 PubMed39134897

2024

Promotion Effect of Catalpol on Angiogenesis and Potential Mechanisms: A Research Based on Network Pharmacology.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Rong Ni,Qun-Hu Zhang,Jie-Lin Deng,Hai-Hu Wang,Yong-Chi Duan,Cheng-Ji Zhang,Lue-Tao Jiang

BMC pulmonary medicine 23:421 PubMed37919693

2023

SOX9 promotes the invasion and migration of lung adenocarcinoma cells by activating the RAP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Fa Yang,Qing Liao,Chen-Lin Lu

Cancer cell 31:685-696.e6 PubMed28486107

2017

RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Chen,Qiuxia Wu,Philippe Depeille,Peirong Chen,Sophie Thornton,Helen Kalirai,Sarah E Coupland,Jeroen P Roose,Boris C Bastian

Nature communications 5:4657 PubMed25118589

2014

RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase.

Applications

Species

Unspecified reactive species

Songqing Tang,Taoyong Chen,Zhou Yu,Xuhui Zhu,Mingjin Yang,Bin Xie,Nan Li,Xuetao Cao,Jianli Wang

Molecular cancer 13:96 PubMed24779681

2014

Function of RasGRP3 in the formation and progression of human breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zsuzsanna Nagy,Ilona Kovács,Miklós Török,Dezső Tóth,György Vereb,Krisztina Buzás,István Juhász,Peter M Blumberg,Tamás Bíró,Gabriella Czifra

PloS one 8:e72331 PubMed23991094

2013

RasGRPs are targets of the anti-cancer agent ingenol-3-angelate.

Applications

Species

Rat

Xiaohua Song,Ana Lopez-Campistrous,Lucy Sun,Nancy A Dower,Noemi Kedei,Jing Yang,Jessica S Kelsey,Nancy E Lewin,Tim E Esch,Peter M Blumberg,James C Stone

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RASGRP3 (phospho T133) antibody [EPNCIR148]

Western blot - Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (AB124823)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (6)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise