Rabbit Polyclonal RASF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASSF1 aa 50 to C-terminus.
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Publications
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- Cell death & disease 11:8552020RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing-Ying Liang,Xu-Bin Deng,Xian-Tao Lin,Li-Li Jiang,Xiao-Ting Huang,Zhi-Wen Mo,Ya-Wei Yuan,Muy-Teck TehPubMed 33057010
- Translational cancer research 9:2721-27332020IL-1β secreted by macrophage M2 promotes metastasis of osteosarcoma via NF-κB/miR-181α-5p/RASSF1A/Wnt pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi-Peng Han,Dong-Biao Liu,Liu-Qing Wu,Qin Li,Zheng-Guang Wang,Xiao-Fang ZangPubMed 35117631
- Surgical oncology 30:63-712019Ras-association domain family 1 (RASSF1A) gene regulates progression, migration and invasion of bladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhai Bao et. al.PubMed 31500788
- Cell proliferation 52:e125482018Circ_0078767 suppresses non-small-cell lung cancer by protecting RASSF1A expression via sponging miR-330-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Chen et. al.PubMed 30507050
- Cell death and differentiation 26:1379-13952018Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjiao Li et. al.PubMed 30315205
- Oncology reports 40:2916-29252018The interaction between miR‑148a and DNMT1 suppresses cell migration and invasion by reactivating tumor suppressor genes in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLe Hong et. al.PubMed 30226601
- Cancer research 75:3411-242015PML/RARα-Regulated miR-181a/b Cluster Targets the Tumor Suppressor RASSF1A in Acute Promyelocytic Leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniela Bräuer-Hartmann et. al.PubMed 26041820
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RASSF1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NS23
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
Alternative names
RDA32, RASSF1, Ras association domain-containing protein 1
Recommended products
Rabbit Polyclonal RASF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASSF1 aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RASSF1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NS23
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-RASSF1 antibody (ab97749)
12% SDS PAGE
All lanes: Western blot - Anti-RASSF1 antibody (ab97749) at 1/500 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 39 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RASSF1 antibody (ab97749)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97749 at 1/100 dilution. Lower image is merged with DNA probe.
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