AB97749

Anti-RASSF1 antibody

Name: Anti-RASSF1 antibody (ab97749) | Abcam
Brand: Abcam Limited
SKU: AB97749
Price: 520 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal RASF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASSF1 aa 50 to C-terminus.

View Alternative Names

RDA32, RASSF1, Ras association domain-containing protein 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-RASSF1 antibody (AB97749)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RASSF1 antibody (AB97749)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97749 at 1/100 dilution. Lower image is merged with DNA probe.

Unknown

Western blot - Anti-RASSF1 antibody (AB97749)

12% SDS PAGE

All lanes:

Western blot - Anti-RASSF1 antibody (ab97749) at 1/500 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RASSF1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NS23

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.

See full target information RASSF1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 11:855 PubMed33057010

2020

RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ying Liang,Xu-Bin Deng,Xian-Tao Lin,Li-Li Jiang,Xiao-Ting Huang,Zhi-Wen Mo,Ya-Wei Yuan,Muy-Teck Teh

Translational cancer research 9:2721-2733 PubMed35117631

2020

IL-1β secreted by macrophage M2 promotes metastasis of osteosarcoma via NF-κB/miR-181α-5p/RASSF1A/Wnt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Peng Han,Dong-Biao Liu,Liu-Qing Wu,Qin Li,Zheng-Guang Wang,Xiao-Fang Zang

Surgical oncology 30:63-71 PubMed31500788

2019

Ras-association domain family 1 (RASSF1A) gene regulates progression, migration and invasion of bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhai Bao,Xuefeng Liu,Yang Liu,Si Wang,Bin Wu

Cell proliferation 52:e12548 PubMed30507050

2018

Circ_0078767 suppresses non-small-cell lung cancer by protecting RASSF1A expression via sponging miR-330-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Chen,Zuozhang Yang,Chao Liu,Li Wang,Jun Yang,Long Chen,Wenhui Li

Cell death and differentiation 26:1379-1395 PubMed30315205

2018

Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjiao Li,Fei Yue,Yuan Dai,Boyun Shi,Guibin Xu,Xianhan Jiang,Xinke Zhou,Gerd P Pfeifer,Leyuan Liu

Oncology reports 40:2916-2925 PubMed30226601

2018

The interaction between miR‑148a and DNMT1 suppresses cell migration and invasion by reactivating tumor suppressor genes in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Le Hong,Gen Sun,Long Peng,Yi Tu,Zhen Wan,Haiwei Xiong,Yong Li,Weidong Xiao

Cancer research 75:3411-24 PubMed26041820

2015

PML/RARα-Regulated miR-181a/b Cluster Targets the Tumor Suppressor RASSF1A in Acute Promyelocytic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Bräuer-Hartmann,Jens-Uwe Hartmann,Alexander Arthur Wurm,Dennis Gerloff,Christiane Katzerke,Maria Vittoria Verga Falzacappa,Pier Giuseppe Pelicci,Carsten Müller-Tidow,Daniel G Tenen,Dietger Niederwieser,Gerhard Behre

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RASSF1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RASSF1 antibody (AB97749)

Western blot - Anti-RASSF1 antibody (AB97749)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise