Anti-RASSF1a antibody [3F3]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-RASSF1a antibody [3F3] (AB23950)

A431 cells were labeled with anti-RASSF1a(clone 3F3) ab23950. Detection was done using a biotinylated secondary antibody and Texas-red conjugated streptavidin.

This image was generated using the ascites version of the product.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RASSF1a antibody [3F3] (AB23950)

Immunocytochemistry/ Immunofluorescence analysis of RASSF1a in HeLa cells. The cell was stained with ab23950 (1 : 100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

• Flow Cyt

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Flow Cytometry - Anti-RASSF1a antibody [3F3] (AB23950)

Overlay histogram showing HeLA cells stained with ab23950 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab23950, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

• WB

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Western blot - Anti-RASSF1a antibody [3F3] (AB23950)

Proteins were visualised using a goat anti-mouse secondary antibody conjugation to HRP and a ECL detection system.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-RASSF1a antibody [3F3] (ab23950) at 1/1000 dilution

Lane 1:

HEK293 cells

Lane 2:

A549 cells

Lane 3:

HeLa cells

Predicted band size: 39 kDa

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• WB

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Western blot - Anti-RASSF1a antibody [3F3] (AB23950)

Arrows indicate RASSF1A protein (about 40kDa in Hela cells, and 43kDa in other tissues from mice).

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-RASSF1a antibody [3F3] (ab23950) at 1/1000 dilution

Lane 1:

Hela (H) cell extract at 20 µg

Lane 2:

Mouse brain (MB) extract at 20 µg

Lane 3:

Mouse liver (ML) extract at 20 µg

Lane 4:

Mouse kidney (MK) extract at 20 µg

Secondary

All lanes:

Goat anti-mouse HRP conjugate

Predicted band size: 39 kDa

Observed band size: 40 kDa,43 kDa

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• WB

Supplier Data

Western blot - Anti-RASSF1a antibody [3F3] (AB23950)

All lanes:

Western blot - Anti-RASSF1a antibody [3F3] (ab23950) at 1/500 dilution

Lane 1:

293T cell lysate at 5 µg

Lane 2:

RASSF1A transfected 293T cell lysate at 5 µg

Secondary

All lanes:

goat anti-mouse secondary antibody conjugated to HRP

Predicted band size: 39 kDa

false