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Rat IgG1, kappa monoclonal [RTK2071] - Isotype control Suitable for Flow Cyt and reacts with samples. Cited in 7 publications.

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Images

Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (AB18412), expandable thumbnail

Publications

  • Oncoimmunology 11:20290702022
    T-SIGn tumor reengineering therapy and CAR T cells synergize in combination therapy to clear human lung tumor xenografts and lung metastases in NSG mice.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Olmo Sonzogni,Daniel E Zak,Maria Stella Sasso,Rochelle Lear,Alice Muntzer,Manuela Zonca,Katy West,Brian R Champion,James B Rottman
    PubMed 35154906
  • Research in pharmaceutical sciences 17:123-1332022
    Comparison of the efficacy of bone morphogenetic protein-4 on differentiation of murine adipose and bone marrow mesenchymal stem cells into primordial germ cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Maryam Hosseinzadeh Shirzeyli,Ali Tayyebiazar,Fereshteh Aliakbari,Fahimeh Ghasemi,Fatemeh Eini,Farhad Hosseinzadeh Shirzeyli,Elmira Vanaki,Aligholi Sobhani
    PubMed 35280837

Key facts

Isotype

IgG1

Host species

Rat

Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Reactivity data

Application

Flow Cyt

Reactivity

Reacts

Dilution info

1 µg for 106 Cells

Notes

-

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      Rat IgG1, kappa monoclonal [RTK2071] - Isotype control Suitable for Flow Cyt and reacts with samples. Cited in 7 publications.

      Key facts

      Isotype

      IgG1

      Form

      Liquid

      Clonality

      Monoclonal

      Clone number

      RTK2071

      Purification technique

      Affinity purification Protein A

      Light chain type

      kappa

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Notes

      The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

      Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

      If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

      Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      1 product image

      • Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412), expandable thumbnail

        Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

        Overlay histogram showing HeLa cells stained with ab16881 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16881, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

        Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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