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AB18412

Rat IgG1, kappa monoclonal [RTK2071] - Isotype control

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(9 Publications)

Rat IgG1, kappa monoclonal [RTK2071] - Isotype control Suitable for Flow Cyt and reacts with samples. Cited in 9 publications.
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Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (AB18412)
  • Flow Cyt

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Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (AB18412)

Overlay histogram showing HeLa cells stained with ab16881 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16881, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Biotin

    Biotin Rat IgG1, kappa [RTK2071] - isotype control

  • 519 FITC

    FITC Rat IgG1, kappa [RTK2071] - isotype control

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RTK2071

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Oncoimmunology 11:2029070 PubMed35154906

2022

T-SIGn tumor reengineering therapy and CAR T cells synergize in combination therapy to clear human lung tumor xenografts and lung metastases in NSG mice.

Applications

Unspecified application

Species

Unspecified reactive species

Olmo Sonzogni,Daniel E Zak,Maria Stella Sasso,Rochelle Lear,Alice Muntzer,Manuela Zonca,Katy West,Brian R Champion,James B Rottman

Research in pharmaceutical sciences 17:123-133 PubMed35280837

2022

Comparison of the efficacy of bone morphogenetic protein-4 on differentiation of murine adipose and bone marrow mesenchymal stem cells into primordial germ cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Hosseinzadeh Shirzeyli,Ali Tayyebiazar,Fereshteh Aliakbari,Fahimeh Ghasemi,Fatemeh Eini,Farhad Hosseinzadeh Shirzeyli,Elmira Vanaki,Aligholi Sobhani

Nature communications 11:635 PubMed32005814

2020

The in vivo genetic program of murine primordial lung epithelial progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Laertis Ikonomou,Michael J Herriges,Sara L Lewandowski,Robert Marsland,Carlos Villacorta-Martin,Ignacio S Caballero,David B Frank,Reeti M Sanghrajka,Keri Dame,Maciej M Kańduła,Julia Hicks-Berthet,Matthew L Lawton,Constantina Christodoulou,Attila J Fabian,Eric Kolaczyk,Xaralabos Varelas,Edward E Morrisey,John M Shannon,Pankaj Mehta,Darrell N Kotton

Artificial cells, nanomedicine, and biotechnology 46:1744-1753 PubMed29199459

2017

Isolation and characterization of mesenchymal stem cells and its antitumor application on ovarian cancer cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Farideh Mohammadian,Babak Negahdari

Microscopy research and technique 80:1151-1160 PubMed28921810

2017

Bones Morphogenic Protein-4 and retinoic acid combined treatment comparative analysis for in vitro differentiation potential of murine mesenchymal stem cells derived from bone marrow and adipose tissue into germ cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam H Shirzeyli,Neda Khanlarkhani,Fardin Amidi,Farshad H Shirzeyli,Fereydoon S Aval,Aligholi Sobhani

Molecular therapy : the journal of the American So 25:2502-2512 PubMed28803863

2017

Epidermal E-Cadherin Dependent β-Catenin Pathway Is Phytochemical Inducible and Accelerates Anagen Hair Cycling.

Applications

Unspecified application

Species

Unspecified reactive species

Noha S Ahmed,Subhadip Ghatak,Mohamed S El Masry,Surya C Gnyawali,Sashwati Roy,Mohamed Amer,Helen Everts,Chandan K Sen,Savita Khanna

Iranian journal of basic medical sciences 19:1186-1192 PubMed27917274

2016

Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mahdieh Hajian Monfared,Bagher Minaee,Tayebeh Rastegar,Ebrahim Khrazinejad,Mohammad Barbarestani

Iranian journal of reproductive medicine 11:965-76 PubMed24639722

2014

Comparison of differentiation potential of male mouse adipose tissue and bone marrow derived-mesenchymal stem cells into germ cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Hosseinzadeh Shirzeily,Parichehr Pasbakhsh,Fardin Amidi,Kobra Mehrannia,Aligholi Sobhani

Journal of cell science 122:849-58 PubMed19240116

2009

A novel role for SED1 (MFG-E8) in maintaining the integrity of the epididymal epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Adam S Raymond,Barry D Shur
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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