AB185799

Rat IgG2a, kappa monoclonal [eBR2a] - Isotype control (Low endotoxin, Azide Free)

Name: Rat IgG2a, kappa monoclonal [eBR2a] - Isotype control (Low endotoxin, Azide Free) (ab185799) | Abcam
Brand: Abcam Limited
SKU: AB185799
Price: 905 USD
Availability: InStock

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Functional Studies (Depl) - Rat IgG2a, kappa monoclonal [eBR2a] - Isotype control (Low endotoxin, Azide Free) (AB185799)

Flow cytometric analysis of peripheral blood samples from neutrophil Ly6G-Tomato reporter mice 48h post intraperitoneal injection with 500μg of anti-Ly6G [1A8] antibody mouse IgG2c (chimeric) (ab320708) (A) or Anti-Ly6g [1A8] antibody – rat IgG2a (ab210204) (B) and corresponding isotype controls.

ab320708 injections result in prolonged neutrophils depletion compared to ab210204.

Cells were stained with CD45 conjugated to PE-Cy7 and CD11b conjugated to APC- eFluor 780. Gated on viable CD45+ population for analysis of CD11+ Ly6G-tdTomato+ neutrophils.

Developed in collaboration with Tatyana Chtanova lab, Garvan Institute of Medical Research and UNSW Sydney, Australia.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

eBR2a

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Applications

Flow Cyt

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Product details

Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by the LAL assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rat IgG2a, kappa monoclonal [eBR2a] - Isotype control (Low endotoxin, Azide Free)

Functional Studies (Depl) - Rat IgG2a, kappa monoclonal [eBR2a] - Isotype control (Low endotoxin, Azide Free) (AB185799)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Applications

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

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