Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 14 publications.
Images
![Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (AB18450), expandable thumbnail](https://content.abcam.com/products/images/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450--flow-cytometry-img32687.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (AB18450)")
Publications
Filter by
- British journal of cancer 128:1742-17522023Comparative analysis of EpCAM high-expressing and low-expressing circulating tumour cells with regard to their clonal relationship and clinical value.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndré Franken,Annika Kraemer,Alicia Sicking,Meike Watolla,Mahdi Rivandi,Liwen Yang,Jens Warfsmann,Bernhard M Polzer,Thomas W P Friedl,Franziska Meier-Stiegen,Nikolas H Stoecklein,Davut Dayan,Sabine Riethdorf,Volkmar Mueller,Klaus Pantel,André Koch,Andreas D Hartkopf,Natalia Krawczyk,Eugen Ruckhaeberle,Dieter Niederacher,Tanja Fehm,Hans NeubauerPubMed 36823365
- Circulation research 128:690-7052021Atherosclerosis Regression and Cholesterol Efflux in Hypertriglyceridemic Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatjana Josefs,Debapriya Basu,Tomas Vaisar,Britt Arets,Jenny E Kanter,Lesley-Ann Huggins,Yunying Hu,Jianhua Liu,Noemie Clouet-Foraison,Jay W Heinecke,Karin E Bornfeldt,Ira J Goldberg,Edward A FisherPubMed 33530703
- FASEB journal : official publication of the Federa 34:7885-79042020Small proline-rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarika Saraswati et. al.PubMed 32297675
- Cell reports 30:555-570.e72020Tissue-Resident PDGFRα Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Paola Santini,Daniela Malide,Gabriel Hoffman,Gaurav Pandey,Valentina D'Escamard,Aya Nomura-Kitabayashi,Ilsa Rovira,Hiroshi Kataoka,Jordi Ochando,Richard P Harvey,Toren Finkel,Jason C KovacicPubMed 31940496
- BMC immunology 20:472019ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPolyxeni T Mantani et. al.PubMed 31823769
- Arteriosclerosis, thrombosis, and vascular biology 39:1967-19852019Role of LpL (Lipoprotein Lipase) in Macrophage Polarization In Vitro and In Vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye Rim Chang et. al.PubMed 31434492
- Molecular therapy : the journal of the American Society of Gene Therapy 27:912-9212019The GPI-Linked Protein LY6A Drives AAV-PHP.B Transport across the Blood-Brain Barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuliette Hordeaux,Yuan Yuan,Peter M Clark,Qiang Wang,R Alexander Martino,Joshua J Sims,Peter Bell,Angela Raymond,William L Stanford,James M WilsonPubMed 30819613
- Cancer letters 432:56-682018The identification of the ATR inhibitor VE-822 as a therapeutic strategy for enhancing cisplatin chemosensitivity in esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Shi et. al.PubMed 29890208
- The Journal of clinical investigation 127:3987-40002017Mast cell hyperactivity underpins the development of oxygen-induced retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenshiro Matsuda et. al.PubMed 28990934
- Biomaterials science 5:223-2332016Effects of ovalbumin protein nanoparticle vaccine size and coating on dendritic cell processing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy Z Chang,Samantha S Stadmiller,Erika Staskevicius,Julie A ChampionPubMed 27918020
Key facts
- Isotype
IgG2a
- Host species
Rat
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IP | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info - | Notes - |
Application WB | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 2 µg for 106 Cells | Notes ab18450 is a negative isotype control. |
Associated Products
Select an associated product type
15 products for Alternative Product
Recommended products
Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 14 publications.
Key facts
- Isotype
IgG2a
- Host species
Rat
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Clone number
RTK2758
- Purification technique
Affinity purification Immunogen
- Light chain type
kappa
- Concentration
- Purification notes
The immunoglobulin was purified by affinity chromatography.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450), expandable thumbnail](https://content.abcam.com/products/images/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450--flow-cytometry-img32687.jpg "Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)")
Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
Overlay histogram showing HeLa cells stained with Anti-MAPRE1/EB1 antibody [KT51] ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-MAPRE1/EB1 antibody [KT51] ab53358, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com