Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control

3 Images

• IP

Lab

Immunoprecipitation - Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (AB18541)

CBX1 / HP1 beta was immunoprecipitated from 0.4 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab10811 at 1/50 dilution (2µg in 0.4mg lysates). Western blot was performed on the immunoprecipitate using ab10811 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-CBX1 / HP1 beta antibody [MAC353] (<a href='/en-us/products/primary-antibodies/cbx1-hp1-beta-antibody-mac353-ab10811'>ab10811</a>) at 1/50 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate (Input) at 10 µg

Lane 2:

HepG2 whole cell lysate

Lane 3:

Rat IgG2b isotype control (ab18541) instead of <a href='/en-us/products/primary-antibodies/cbx1-hp1-beta-antibody-mac353-ab10811'>ab10811</a> in HepG2 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 21 kDa

false

Exposure time: 1s

• IP

Lab

Immunoprecipitation - Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (AB18541)

CBX1 / HP1 beta was immunoprecipitated from 0.4 mg ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) whole cell lysate with ab10811 at 1/50 dilution (2µg in 0.4mg lysates). Western blot was performed on the immunoprecipitate using ab10811 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-CBX1 / HP1 beta antibody [MAC353] (<a href='/en-us/products/primary-antibodies/cbx1-hp1-beta-antibody-mac353-ab10811'>ab10811</a>) at 1/50 dilution

Lane 1:

ES-D3 [D3]  (mouse blastocyst-derived embryonic stem cell) whole cell lysate (Input) at 10 µg

Lane 2:

ES-D3 [D3]  (mouse blastocyst-derived embryonic stem cell) whole cell lysate (+)

Lane 3:

Rat IgG2b isotype control (ab18541) instead of <a href='/en-us/products/primary-antibodies/cbx1-hp1-beta-antibody-mac353-ab10811'>ab10811</a> in ES-D3 [D3] whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 21 kDa

false

Exposure time: 1s

• IP

Lab

Immunoprecipitation - Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (AB18541)

Strep Tag II was immunoprecipitated from HEK-293T transfected with GFP expression vector containing a Strep Tag II whole cell lysate with ab252885 at 1/50 dilution. Western blot was performed on the immunoprecipitate using ab252885 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/10000 dilution. Lane 1(Input) : HEK-293T transfected with GFP expression vector containing a strep tag whole cell lysate 2 μg Lane 2 (+) : HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate Lane 3 (-) : Rat monoclonal IgG2b (ab18541) instead of ab252885 in HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate Observed MV : 26 kDa Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Strep-tag II antibody [11A7] (<a href='/en-us/products/primary-antibodies/strep-tag-ii-antibody-11a7-ab252885'>ab252885</a>) at 1/1000 dilution

Lane 1:

HEK-293T transfected with GFP expression vector containing a strep tag whole cell lysate at 2 µg

Lane 2:

HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate

Lane 3:

Rat monoclonal IgG2b (ab18541) instead of <a href='/en-us/products/primary-antibodies/strep-tag-ii-antibody-11a7-ab252885'>ab252885</a> in HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Observed band size: 26 kDa

false

Exposure time: 5s