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Rat IgM, kappa monoclonal [RTK2118] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 2 publications.

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Images

Flow Cytometry - Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (AB35768), expandable thumbnail

Publications

Key facts

Isotype

IgM

Host species

Rat

Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Reactivity data

Application

IP

Reactivity

Expected

Dilution info

-

Notes

-

Application

IHC-Fr

Reactivity

Expected

Dilution info

-

Notes

-

Application

ICC/IF

Reactivity

Expected

Dilution info

-

Notes

-

Application

WB

Reactivity

Expected

Dilution info

-

Notes

-

Application

IHC-P

Reactivity

Expected

Dilution info

-

Notes

-

Application

Flow Cyt

Reactivity

Reacts

Dilution info

2 µg for 106 Cells

Notes

ab35768 is a negative isotype control

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Rat IgM, kappa monoclonal [RTK2118] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 2 publications.

Key facts

Isotype

IgM

Form

Liquid

Clonality

Monoclonal

Clone number

RTK2118

Purity

IgM fraction

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Flow Cytometry - Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (ab35768), expandable thumbnail

    Flow Cytometry - Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (ab35768)

    Overlay histogram showing RAMOS cells stained with ab19795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19795, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat kappa monoclonal [RTK2118] (ab35768, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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