JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB35768

Rat IgM, kappa monoclonal [RTK2118] - Isotype Control

Be the first to review this product! Submit a review

|

(4 Publications)

Rat IgM, kappa monoclonal [RTK2118] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 4 publications.
1 Images
Flow Cytometry - Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (AB35768)
  • Flow Cyt

Unknown

Flow Cytometry - Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (AB35768)

Overlay histogram showing RAMOS cells stained with ab19795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19795, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat kappa monoclonal [RTK2118] (ab35768, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • 519 FITC

    FITC Rat IgM, kappa monoclonal [RTK2118] - Isotype control

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RTK2118

Isotype

IgM

Light chain type

kappa

Carrier free

No

Applications

ICC/IF, IHC-Fr, IHC-P, Flow Cyt, IP, WB

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "IP-species-checked": "", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCFr-species-checked": "", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p>ab35768 is a negative isotype control</p>" } } }
style
left-column

Product details

Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies.
style
left-column

Properties and storage information

Form
Liquid
Purity
IgM fraction
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Contrast media & molecular imaging 2018:8092781 PubMed29681783

2018

Imaging and Methotrexate Response Monitoring of Systemic Inflammation in Arthritic Rats Employing the Macrophage PET Tracer [F]Fluoro-PEG-Folate.

Applications

Unspecified application

Species

Unspecified reactive species

Durga M S H Chandrupatla,Gerrit Jansen,Elise Mantel,Philip S Low,Takami Matsuyama,René P Musters,Albert D Windhorst,Adriaan A Lammertsma,Carla F M Molthoff,Conny J van der Laken

Virulence 8:1290-1302 PubMed28402705

2017

Interaction of factor H-binding protein of Streptococcus suis with globotriaosylceramide promotes the development of meningitis.

Applications

Unspecified application

Species

Unspecified reactive species

Decong Kong,Zhe Chen,Junping Wang,Qingyu Lv,Hua Jiang,Yuling Zheng,Maokai Xu,Xuyu Zhou,Huaijie Hao,Yongqiang Jiang

American journal of physiology. Renal physiology 311:F1015-F1024 PubMed27681560

2016

Characterization and phosphoproteomic analysis of a human immortalized podocyte model of Fabry disease generated using CRISPR/Cas9 technology.

Applications

Unspecified application

Species

Unspecified reactive species

Ester M Pereira,Anatália Labilloy,Megan L Eshbach,Ankita Roy,Arohan R Subramanya,Semiramis Monte,Guillaume Labilloy,Ora A Weisz

Frontiers in cell and developmental biology 3:15 PubMed25806369

2015

Epigenetic reprogramming and re-differentiation of a Ewing sarcoma cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph B Moore,David M Loeb,Kyung U Hong,Poul H Sorensen,Timothy J Triche,David W Lee,Michael I Barbato,Robert J Arceci
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB37361

Rat IgG - Isotype Control

  • 0
  • 0
Primary Antibodies

AB18412

Rat IgG1, kappa monoclonal [RTK2071] - Isotype control

Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (AB18412)

  • 0
  • 0
Primary Antibodies

AB18450

Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control

Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (AB18450)

  • 0
  • 0
Primary Antibodies

AB18541

Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control

Immunoprecipitation - Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (AB18541)

  • 0
  • 0
Primary Antibodies

AB18446

FITC Rat IgG2a, kappa monoclonal [RTK2758] - Isotype control

  • 0
  • 0

Complementary Products

Primary Antibodies

AB18412

Rat IgG1, kappa monoclonal [RTK2071] - Isotype control

Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (AB18412)

  • 0
  • 0
Primary Antibodies

AB18401

Mouse IgM, kappa monoclonal [MM-30] - isotype control

  • 0
  • 0
Primary Antibodies

AB18479

Armenian Hamster IgG monoclonal [HTK888] - Isotype Control

  • 0
  • 0
Primary Antibodies

AB212201

Anti-Human IgM antibody [EPR20731]

RabMAb|Recombinant

Flow Cytometry - Anti-Human IgM antibody [EPR20731] (AB212201)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com