Rat IgM, kappa monoclonal [RTK2118] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 2 publications.
IgM
Rat
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS
Liquid
Monoclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application IP | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info - | Notes - |
Application WB | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 2 µg for 106 Cells | Notes ab35768 is a negative isotype control |
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Rat IgM, kappa monoclonal [RTK2118] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 2 publications.
IgM
Rat
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS
Liquid
Monoclonal
RTK2118
IgM fraction
kappa
Blue Ice
+4°C
+4°C
Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies.
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Overlay histogram showing RAMOS cells stained with ab19795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19795, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat kappa monoclonal [RTK2118] (ab35768, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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