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Rat Recombinant Monoclonal C9orf72 antibody. Suitable for WB, IHC-P, ICC/IF, I-ELISA and reacts with Transfected cell lysate - Human, Transfected cell line - Human, Synthetic peptide - Human samples.

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Images

Indirect ELISA - Anti-Rat Poly PR antibody [PERA534G] (AB323367), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Rat Poly PR antibody [PERA534G] (AB323367), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rat Poly PR antibody [PERA534G] (AB323367), expandable thumbnail
  • Western blot - Anti-Rat Poly PR antibody [PERA534G] (AB323367), expandable thumbnail

Key facts

Isotype
IgG2c
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFI-ELISA
Human
Predicted
Predicted
Predicted
Predicted
Synthetic peptide - Human
Not recommended
Not recommended
Not recommended
Tested
Transfected cell line - Human
Not recommended
Tested
Tested
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/500
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
500 ng/mL
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Transfected cell line - Human
Dilution info
-
Notes

-

Target data

Function

Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy (PubMed:27103069, PubMed:27193190, PubMed:27617292, PubMed:28195531, PubMed:32303654). In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation (PubMed:27103069). The C9orf72-SMCR8 complex also acts as a regulator of autophagy initiation by interacting with the ULK1/ATG1 kinase complex and modulating its protein kinase activity (PubMed:27617292). As part of the C9orf72-SMCR8 complex, stimulates RAB8A and RAB11A GTPase activity in vitro (PubMed:32303654). Positively regulates initiation of autophagy by regulating the RAB1A-dependent trafficking of the ULK1/ATG1 kinase complex to the phagophore which leads to autophagosome formation (PubMed:27334615). Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates (PubMed:27559131). Plays a role in endosomal trafficking (PubMed:24549040). May be involved in regulating the maturation of phagosomes to lysosomes (By similarity). Promotes the lysosomal localization and lysosome-mediated degradation of CARM1 which leads to inhibition of starvation-induced lipid metabolism (By similarity). Regulates actin dynamics in motor neurons by inhibiting the GTP-binding activity of ARF6, leading to ARF6 inactivation (PubMed:27723745). This reduces the activity of the LIMK1 and LIMK2 kinases which are responsible for phosphorylation and inactivation of cofilin, leading to CFL1/cofilin activation (PubMed:27723745). Positively regulates axon extension and axon growth cone size in spinal motor neurons (PubMed:27723745). Required for SMCR8 protein expression and localization at pre- and post-synaptic compartments in the forebrain, also regulates protein abundance of RAB3A and GRIA1/GLUR1 in post-synaptic compartments in the forebrain and hippocampus (By similarity). Plays a role within the hematopoietic system in restricting inflammation and the development of autoimmunity (By similarity). Isoform 1. Regulates stress granule assembly in response to cellular stress. Isoform 2. Does not play a role in regulation of stress granule assembly in response to cellular stress.

Alternative names

DENND9, DENNL72, C9orf72, Guanine nucleotide exchange factor C9orf72

Rat Recombinant Monoclonal C9orf72 antibody. Suitable for WB, IHC-P, ICC/IF, I-ELISA and reacts with Transfected cell lysate - Human, Transfected cell line - Human, Synthetic peptide - Human samples.

Key facts

Isotype
IgG2c
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
PERA534G
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Indirect ELISA - Anti-Rat Poly PR antibody [PERA534G] (ab323367), expandable thumbnail

    Indirect ELISA - Anti-Rat Poly PR antibody [PERA534G] (ab323367)

    Indirect ELISA analysis of ab323367 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) at 1:1000 dilution dilution.

    Antigen: poly (PR)20 peptide.

    Antigen concentration: 1000 ng/ml

  • Immunocytochemistry/ Immunofluorescence - Anti-Rat Poly PR antibody [PERA534G] (ab323367), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Rat Poly PR antibody [PERA534G] (ab323367)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Poly PR with ab323367 at 1/500 (1.91 ug/ml) dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing nuclear with cytoplasmic staining in 293T cells transfected with a poly PR expression vector containing a EGFP-tag®(shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    was used to counterstain tubulin at 1/None dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) at 1/1000 2ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rat Poly PR antibody [PERA534G] (ab323367), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rat Poly PR antibody [PERA534G] (ab323367)

    Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T (human embryonic kidney epithelial cell) transfected with a poly PR expression vector containing a EGFP-tag. Panel B HEK-293T transfected with empty vector containing a EGFP-tag. tissue labeling Poly PR with ab323367 at 1/5000 (0.217 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) HEK-293T transfected with a poly PR expression vector, no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab323367 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-Rat Poly PR antibody [PERA534G] (ab323367), expandable thumbnail

    Western blot - Anti-Rat Poly PR antibody [PERA534G] (ab323367)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    In Western blot, Anti-GFP antibody [E385] (Anti-GFP antibody [E385] ab32146) staining at 35kDa dilution.

    All lanes: Western blot - Anti-Rat Poly PR antibody [PERA534G] (ab323367) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containi a EGPF-tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a poly (PR)20 expression vector containi a EGFP-tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/20000 dilution

    Observed band size: 35 kDa, 124 kDa

    Exposure time: 1s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com