Rabbit Polyclonal RB antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RB1.
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- Communications biology 5:10362022Interruption of aberrant chromatin looping is required for regenerating RB1 function and suppressing tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuyang Wen,Tianyi Ding,Fang Li,Jiayan Fan,Xianqun Fan,Renbing Jia,He ZhangPubMed 36175480
- Environmental toxicology 36:1785-17922021Arsenic trioxide reduces the expression of E2F1, cyclin E, and phosphorylation of PI3K signaling molecules in acute leukemia cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanjay Kumar,Paul B TchounwouPubMed 34042274
- Cancer discovery 11:2334-23532021RB/E2F1 as a Master Regulator of Cancer Cell Metabolism in Advanced Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy C Mandigo,Wei Yuan,Kexin Xu,Peter Gallagher,Angel Pang,Yi Fang Guan,Ayesha A Shafi,Chellappagounder Thangavel,Beshara Sheehan,Denisa Bogdan,Alec Paschalis,Jennifer J McCann,Talya S Laufer,Nicolas Gordon,Irina A Vasilevskaya,Emanuela Dylgjeri,Saswati N Chand,Matthew J Schiewer,Josep Domingo-Domenech,Robert B Den,Jeff Holst,Peter A McCue,Johann S de Bono,Christopher McNair,Karen E KnudsenPubMed 33879449
- Cellular physiology and biochemistry : internation 51:1149-11632018Long Non-Coding RNA Urothelial Carcinoma Associated 1 Promotes Proliferation, Migration and Invasion of Osteosarcoma Cells by Regulating microRNA-182.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiang Li et. al.PubMed 30481751
- International journal of oncology 52:1827-18402018β‑catenin nuclear translocation induced by HIF‑1α overexpression leads to the radioresistance of prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Luo et. al.PubMed 29658569
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RB1. The exact immunogen used to generate this antibody is proprietary information. Database link P06400
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Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Alternative names
Retinoblastoma-associated protein, p105-Rb, p110-RB1, pRb, pp110, Rb, RB1
Recommended products
Rabbit Polyclonal RB antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RB1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RB1. The exact immunogen used to generate this antibody is proprietary information. Database link P06400
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Retinoblastoma protein (Rb) also known as pRb is an important regulatory protein with a molecular weight of approximately 110 kDa. It is mainly expressed in the nucleus of cells. Rb functions as a tumor suppressor by controlling the cell cycle progression from G1 to S phase. Rb becomes active when it is dephosphorylated allowing it to bind and inhibit E2F transcription factors consequently preventing the transcription of genes essential for S phase entry.
- Biological function summary
Retinoblastoma protein influences cellular proliferation differentiation and apoptosis. It acts within a larger protein complex modulating various cellular responses. When functional Rb halts uncontrolled cell division important for maintaining normal tissue homeostasis. In its phosphorylated state known as phospho-Rb or phospho-Rb E182 it loses its regulatory capabilities which can lead to unrestrained cell cycle progression.
- Pathways
Several involve the retinoblastoma protein. One key pathway is the p53 pathway which Rb interacts with to influence cellular outcomes like cell-cycle arrest and apoptosis. Rb cooperates with proteins like p21 to implement these processes. Additionally it is involved in the cyclin D-dependent kinase 4 (CDK4) pathway which modulates its phosphorylation state influencing binding interactions and cell cycle control.
- Associated diseases and disorders
Dysregulation of retinoblastoma protein is commonly associated with cancer particularly retinoblastoma and breast cancer. In retinoblastoma mutations in the Rb gene directly lead to uncontrolled cell proliferation due to the absence of functional Rb protein. Additionally Rb's connection to the E2F family of transcription factors can become disrupted contributing to oncogenesis in other cancers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb antibody (ab226979)
Paraffin-embedded human colon carcinoma tissue stained for Rb using ab226979 at 1/500 dilution in immunohistochemical analysis.
Immunoprecipitation - Anti-Rb antibody (ab226979)
Rb was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg of ab226979. Western blot was performed from the immunoprecipitate using ab226979 at 1/500 dilution.
Lane 1: Rabbit IgG instead of ab226979 in HeLa whole cell lysate.
Lane 2: ab226979 IP in HeLa whole cell lysate.
All lanes: Immunoprecipitation - Anti-Rb antibody (ab226979)
Predicted band size: 106 kDa
Western blot - Anti-Rb antibody (ab226979)
5% SDS-PAGE gel.
All lanes: Western blot - Anti-Rb antibody (ab226979) at 1/500 dilution
All lanes: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 106 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb antibody (ab226979)
4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for Rb using ab226979 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).
Western blot - Anti-Rb antibody (ab226979)
Western blot: Anti-RB1 antibody (ab226979) staining at 1/1500 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab226979 was shown to bind specifically to RB1. A band was observed at 120 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in RB1 knockout cell line. To generate this image, wild-type and RB1 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Rb antibody (ab226979) at 1/1500 dilution
Lane 1: Wild-type HCT 116 cell lysate at 20 µg
Lanes 1 - 4: Western blot - Human RB1 knockout HCT116 cell line (Human RB1 knockout HCT116 cell line ab286657)
Lane 2: RB1 knockout HCT 116 cell lysate at 20 µg
Lane 3: Wild-type A549 ab288558 cell lysate at 20 µg
Lane 4: RB1 knockout A549 Human RB1 knockout A549 cell line ab286470 cell lysate at 20 µg
SecondaryLanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 120 kDa
Western blot - Anti-Rb antibody (ab226979)
Western blot: Anti-RB1 antibody (ab226979) staining at 1/1500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab226979 was shown to bind specifically to RB1. A band was observed at 105 kDa in wild-type A549 cell lysates with no signal observed at this size in RB1 knockout cell line. To generate this image, wild-type and RB1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Rb antibody (ab226979) at 1/1500 dilution
Lane 1: Wild-type A549 cell lysate at 40 µg
Lane 2: RB1 knockout A549 cell lysate at 40 µg
Lane 3: Wild-type HAP1 cell lysate at 20 µg
Lane 4: RB1 knockout HAP1 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
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