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AB4788

Anti-Rb (phospho S249) antibody

5

(1 Review)

|

(4 Publications)

Rabbit Polyclonal RB phospho S249 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human RB1 phospho S249.

View Alternative Names

Retinoblastoma-associated protein, p105-Rb, p110-RB1, pRb, pp110, Rb, RB1

1 Images
Western blot - Anti-Rb (phospho S249) antibody (AB4788)
  • WB

Unknown

Western blot - Anti-Rb (phospho S249) antibody (AB4788)

Peptide Competition : Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were incubated with 0.50 μg/mL ab4788, following prior incubation in the absence of the phosphopeptide immunogen (1), a generic phosphoserine containing peptide (2), a generic phosphothreonine containing peptide (3), the non-phosphopeptide corresponding to the phosphopeptide immunogen (4), or the presence of the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, therefore demonstrating the specificity of the ab4788 antibody for this epitope. Peptide Competition : Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 1

All lanes:

Western blot - Anti-Rb (phospho S249) antibody (ab4788)

Predicted band size: 106 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human RB1 phospho S249. The exact immunogen used to generate this antibody is proprietary information.

P06400

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Rb protein. The final product is generated by affinity chromatography using a Rb-derived peptide that is phosphorylated at serine 249 and threonine 252.
Storage buffer
pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Retinoblastoma protein (Rb) also known as pRb is an important regulatory protein with a molecular weight of approximately 110 kDa. It is mainly expressed in the nucleus of cells. Rb functions as a tumor suppressor by controlling the cell cycle progression from G1 to S phase. Rb becomes active when it is dephosphorylated allowing it to bind and inhibit E2F transcription factors consequently preventing the transcription of genes essential for S phase entry.
Biological function summary

Retinoblastoma protein influences cellular proliferation differentiation and apoptosis. It acts within a larger protein complex modulating various cellular responses. When functional Rb halts uncontrolled cell division important for maintaining normal tissue homeostasis. In its phosphorylated state known as phospho-Rb or phospho-Rb E182 it loses its regulatory capabilities which can lead to unrestrained cell cycle progression.

Pathways

Several involve the retinoblastoma protein. One key pathway is the p53 pathway which Rb interacts with to influence cellular outcomes like cell-cycle arrest and apoptosis. Rb cooperates with proteins like p21 to implement these processes. Additionally it is involved in the cyclin D-dependent kinase 4 (CDK4) pathway which modulates its phosphorylation state influencing binding interactions and cell cycle control.

Dysregulation of retinoblastoma protein is commonly associated with cancer particularly retinoblastoma and breast cancer. In retinoblastoma mutations in the Rb gene directly lead to uncontrolled cell proliferation due to the absence of functional Rb protein. Additionally Rb's connection to the E2F family of transcription factors can become disrupted contributing to oncogenesis in other cancers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed : 10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed : 10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed : 10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed : 10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed : 15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).. (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
See full target information RB1 phospho S249
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Ponce Health Sciences University scientific journal 1: PubMed41050593

2025

Biomarkers for Prostate Cancer Aggressiveness in Puerto Rican Men: Analysis of Phospho-Rb S249, N-cadherin, β-catenin, and E-cadherin Expression in Prostate Biopsies.

Applications

Unspecified application

Species

Unspecified reactive species

Sheila M Valle Cortés,Jaileene Pérez Morales,Mariely Nieves Plaza,Raymond Quiñones Alvarado,Gilberto Ruiz Deyá,Juan C Santa Rosario,Pedro Santiago Cardona

Stats 7:875-893 PubMed41050870

2024

Integrating Proteomic Analysis and Machine Learning to Predict Prostate Cancer Aggressiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Sheila M Valle Cortés,Jaileene Pérez Morales,Mariely Nieves Plaza,Darielys Maldonado,Swizel M Tevenal Baez,Marc A Negrón Blas,Cayetana Lazcano Etchebarne,José Feliciano,Gilberto Ruiz Deyá,Juan C Santa Rosario,Pedro Santiago Cardona

PloS one 13:e0207483 PubMed30452490

2018

Hyper-phosphorylation of Rb S249 together with CDK5R2/p39 overexpression are associated with impaired cell adhesion and epithelial-to-mesenchymal transition: Implications as a potential lung cancer grading and staging biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Jaileene Pérez-Morales,Darielys Mejías-Morales,Stephanie Rivera-Rivera,Jonathan González-Flores,Mónica González-Loperena,Fernando Y Cordero-Báez,Wilfredo M Pedreira-García,Camille Chardón-Colón,Jennifer Cabán-Rivera,W Douglas Cress,Edna R Gordian,Teresita Muñoz-Antonia,Mauricio Cabrera-Ríos,Angel Isidro,Domenico Coppola,Marilin Rosa,Theresa A Boyle,Victoria Izumi,John M Koomen,Pedro G Santiago-Cardona

Toxicology 249:116-22 PubMed18538911

2008

Macrophage inflammatory protein-3alpha influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells.

Applications

WB

Species

Human

Ying Chu Lee,Tzeon-Jye Chiou,Woan-Fang Tzeng,Sin Tak Chu
View all publications
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