Rabbit Recombinant Monoclonal RB phospho S780 antibody. Suitable for IHC-P, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 17 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (AB173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19783.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (AB173289)")
![Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (AB173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--western-blot-img12430.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (AB173289)")
![Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (AB173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--western-blot-img21010.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (AB173289)")
![Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (AB173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--western-blot-img10358.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (AB173289)")
![Dot Blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (AB173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--dot-blot-img13263.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (AB173289)")
Publications
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- Journal of experimental & clinical cancer research : CR 42:2282023Deubiquitylase YOD1 regulates CDK1 stability and drives triple-negative breast cancer tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhitao Han,Qi Jia,Jing Zhang,Miaomiao Chen,Lining Wang,Kai Tong,Weiwei He,Yajie Zhang,Weina Zhu,Ju Qin,Tao Wang,Tielong Liu,Yong Ma,Yuanming Chen,Siluo Zha,Chunlei ZhangPubMed 37667382
- Science advances 8:eadc89112022ACTR5 controls CDKN2A and tumor progression in an INO80-independent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaobao Xu,Anthony K N Chan,Mingli Li,Qiao Liu,Nicole Mattson,Sheela Pangeni Pokharel,Wen-Han Chang,Yate-Ching Yuan,Jinhui Wang,Roger E Moore,Patrick Pirrotte,Jun Wu,Rui Su,Markus Müschen,Steven T Rosen,Jianjun Chen,Lu Yang,Chun-Wei ChenPubMed 36563143
- PeerJ 10:e137592022JMJD3 suppresses tumor progression in oral tongue squamous cell carcinoma patients receiving surgical resection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYen-Hao Chen,Chang-Han Chen,Chih-Yen Chien,Yan-Ye Su,Sheng-Dean Luo,Shau-Hsuan LiPubMed 35855897
- Cell death & disease 13:3502022Targeted CUL4A inhibition synergizes with cisplatin to yield long-term survival in models of head and neck squamous cell carcinoma through a DDB2-mediated mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrace M Jones,Claudia M Espitia,Aikseng Ooi,Julie E Bauman,Jennifer S Carew,Steffan T NawrockiPubMed 35428778
- Nature communications 12:72972021Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Ting Yen,May Chien,Pei-Yi Wu,Chi-Chang Ho,Chun-Te Ho,Kevin Chih-Yang Huang,Shu-Fen Chiang,K S Clifford Chao,William Tzu-Liang Chen,Shih-Chieh HungPubMed 34911954
- Oncogene 40:4425-44392021Combined inhibition of DDR1 and CDK4/6 induces synergistic effects in ER-positive, HER2-negative breast cancer with PIK3CA/AKT1 mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaryam Shariati,Kurt W Evans,Xiaofeng Zheng,Christopher A Bristow,Patrick Kwok-Shing Ng,Yasmeen Q Rizvi,Coya Tapia,Fei Yang,Alessandro Carugo,Timothy P Heffernan,Michael D Peoples,Debu Tripathy,Funda Meric-BernstamPubMed 34108622
- Life (Basel, Switzerland) 11:2021Spatio-Temporal Expression Pattern of Ki-67, pRB, MMP-9 and Bax in Human Secondary Palate Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTanja Šimić Bilandžija,Katarina Vukojević,Anka Ćorić,Ivna Vuković Kekez,Ivana Medvedec Mikić,Lidija Lasić Arapović,Natalija Filipović,Jasminka Anđelić,Mirna Saraga-Babić,Danijela Kalibović GovorkoPubMed 33672637
- Journal of experimental & clinical cancer research 40:482021The circular RNA circZFR phosphorylates Rb promoting cervical cancer progression by regulating the SSBP1/CDK2/cyclin E1 complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingyi Zhou et. al.PubMed 33516252
- Cell death & disease 11:2192020Pan-cancer analysis reveals synergistic effects of CDK4/6i and PARPi combination treatment in RB-proficient and RB-deficient breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSongyu Li et. al.PubMed 32249776
- Oncology reports 42:1101-11092019Antipsychotic agent pimozide promotes reversible proliferative suppression by inducing cellular quiescence in liver cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Jie Chen et. al.PubMed 31322218
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | Dot | WB | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Expected | Tested |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Alternative names
Retinoblastoma-associated protein, p105-Rb, p110-RB1, pRb, pp110, Rb, RB1
Recommended products
Rabbit Recombinant Monoclonal RB phospho S780 antibody. Suitable for IHC-P, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 17 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR182(N)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Retinoblastoma protein (Rb) also known as pRb is an important regulatory protein with a molecular weight of approximately 110 kDa. It is mainly expressed in the nucleus of cells. Rb functions as a tumor suppressor by controlling the cell cycle progression from G1 to S phase. Rb becomes active when it is dephosphorylated allowing it to bind and inhibit E2F transcription factors consequently preventing the transcription of genes essential for S phase entry.
- Biological function summary
Retinoblastoma protein influences cellular proliferation differentiation and apoptosis. It acts within a larger protein complex modulating various cellular responses. When functional Rb halts uncontrolled cell division important for maintaining normal tissue homeostasis. In its phosphorylated state known as phospho-Rb or phospho-Rb E182 it loses its regulatory capabilities which can lead to unrestrained cell cycle progression.
- Pathways
Several involve the retinoblastoma protein. One key pathway is the p53 pathway which Rb interacts with to influence cellular outcomes like cell-cycle arrest and apoptosis. Rb cooperates with proteins like p21 to implement these processes. Additionally it is involved in the cyclin D-dependent kinase 4 (CDK4) pathway which modulates its phosphorylation state influencing binding interactions and cell cycle control.
- Associated diseases and disorders
Dysregulation of retinoblastoma protein is commonly associated with cancer particularly retinoblastoma and breast cancer. In retinoblastoma mutations in the Rb gene directly lead to uncontrolled cell proliferation due to the absence of functional Rb protein. Additionally Rb's connection to the E2F family of transcription factors can become disrupted contributing to oncogenesis in other cancers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19783.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rb (phospho S780) with ab173289 at 1/100 dilution,followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nucleus staining on normal human colon.
Counter stained with Hematoxylin.
Secondary antibody only control:Used PBS instead of primary antibody,secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--western-blot-img12430.jpg "Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289) at 1/50000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) serum starved for 44 h. Whole cell lysates at 15 µg
Lane 2: Jurkat (Human T cell leukemia T lymphocyte) serum starved for 24 h. Then 10% FBS incubated for 20 h. Whole cell lysates at 15 µg
Lane 3: Jurkat (Human T cell leukemia T lymphocyte) serum starved for 24 h. Then 10% FBS incubated for 20 h. Whole cell lysates Then the membrane was incubated with phosphatase. at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 1min
![Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--western-blot-img21010.jpg "Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)
Blocking/Dilution buffer:5% NFDM/TBST
Exposure time:1 minute
All lanes: Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289) at 1/20000 dilution
Lane 1: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg
Lane 2: K562(Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, serum starved for 2 days at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 106 kDa
![Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--western-blot-img10358.jpg "Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)
Blocking/Dilution buffer:NFDM/TBST
ab173289: anti- Rb (phospho S780);
Anti-Rb (phospho S807) antibody [EPR17732] ab184796: anti- Rb (phospho S807);
Anti-Rb antibody [EPR17512] ab181616: anti-Rb
All lanes: Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289) at 1/5000 dilution
Lane 1: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg
Lane 2: Calyculin A treated K562(Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 106 kDa
![Dot Blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--dot-blot-img13263.jpg "Dot Blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
Dot Blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)
Dot blot analysis of Rb (phospho S780) peptide (Lane 1),Rb(non-phospho) peptide (Lane 2) with ab173289 at a dilution of 1/1000.Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/1000.
Blocking and dilution buffer:5% NFDM/TBST.
Exposure time:3 minutes
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130134.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)
Immunohistochemical analysis of paraffin-embedded Lung squamous cell carcinoma labeling Rb (phospho S780) with ab173289 at 1/100 dilution,followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nucleus staining on tumor cells of lung squamous cell carcinoma.
Counter stained with Hematoxylin.
Secondary antibody only control:Used PBS instead of primary antibody,secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--western-blot-img105059.jpg "Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)
All lanes: Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: Jurkat cell lysate treated with Lambda Phosphatase at 10 µg
Predicted band size: 106 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77690.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)
Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Rb with ab173289 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72844.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Rb with ab173289 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130134.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
![Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--western-blot-img105059.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77690.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289), expandable thumbnail](https://content.abcam.com/products/images/rb-phospho-s780-antibody-epr182n-ab173289--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72844.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)")
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