JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277775

Anti-Rb (phospho S788) antibody [6HCLC]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Multiclonal RB phospho S788 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RB1 phospho S788 aa 750-800.

View Alternative Names

Retinoblastoma-associated protein, p105-Rb, p110-RB1, pRb, pp110, Rb, RB1

2 Images
Immunocytochemistry - Anti-Rb (phospho S788) antibody [6HCLC] (AB277775)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Rb (phospho S788) antibody [6HCLC] (AB277775)

For immunofluorescence analysis COLO 205 cells were fixed and permeabilized for detection of endogenous RbpS788 using Anti-RbpS788 Recombinant Rabbit Multiclonal Antibody (ab277775, 2 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI, and Alexa Fluor® 555 Rhodamine Phalloidin (1/300) was used for cytoskeletal F-actin (red) staining. Detection and localization of RbpS788 (green) in the nucleus can be clearly observed in cells treated with Nocodazole (3 uM, 16 h) as compared to untreated cells. Antibody specificity was demonstrated by competition with the RbpS788 peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.

Western blot - Anti-Rb (phospho S788) antibody [6HCLC] (AB277775)
  • WB

Supplier Data

Western blot - Anti-Rb (phospho S788) antibody [6HCLC] (AB277775)

All lanes:

Western blot - Anti-Rb (phospho S788) antibody [6HCLC] (ab277775) at 2 µg/mL

Lane 1:

Colo 205 (human colon adenocarcinoma cell line) whole cell extracts at 30 µg

Lane 2:

Colo 205 (treated with 3 uM Nocodazole for 24 hours) whole cell extract at 30 µg

Lane 3:

Colo 205 whole cell extract (+ 10 uM phosphopeptide)

Lane 4:

Colo 205 (Nocozadole-treated) whole cell extract (+ 10 uM phosphopeptide) at 30 µg

Predicted band size: 106 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

6HCLC

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human RB1 phospho S788 aa 750-800. The exact immunogen used to generate this antibody is proprietary information.

P06400

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB124821

Anti-Cdk6 antibody [EPR4515]

KO Validated|RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody [EPR4515] (AB124821)

  • 5
  • 2
Primary Antibodies

AB81299

Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB81299)

  • 4
  • 17
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Retinoblastoma protein (Rb) also known as pRb is an important regulatory protein with a molecular weight of approximately 110 kDa. It is mainly expressed in the nucleus of cells. Rb functions as a tumor suppressor by controlling the cell cycle progression from G1 to S phase. Rb becomes active when it is dephosphorylated allowing it to bind and inhibit E2F transcription factors consequently preventing the transcription of genes essential for S phase entry.
Biological function summary

Retinoblastoma protein influences cellular proliferation differentiation and apoptosis. It acts within a larger protein complex modulating various cellular responses. When functional Rb halts uncontrolled cell division important for maintaining normal tissue homeostasis. In its phosphorylated state known as phospho-Rb or phospho-Rb E182 it loses its regulatory capabilities which can lead to unrestrained cell cycle progression.

Pathways

Several involve the retinoblastoma protein. One key pathway is the p53 pathway which Rb interacts with to influence cellular outcomes like cell-cycle arrest and apoptosis. Rb cooperates with proteins like p21 to implement these processes. Additionally it is involved in the cyclin D-dependent kinase 4 (CDK4) pathway which modulates its phosphorylation state influencing binding interactions and cell cycle control.

Dysregulation of retinoblastoma protein is commonly associated with cancer particularly retinoblastoma and breast cancer. In retinoblastoma mutations in the Rb gene directly lead to uncontrolled cell proliferation due to the absence of functional Rb protein. Additionally Rb's connection to the E2F family of transcription factors can become disrupted contributing to oncogenesis in other cancers.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed : 10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed : 10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed : 10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed : 10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed : 15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).. (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
See full target information RB1 phospho S788
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:1164-1174 PubMed39930848

2025

suppresses expression to promote cardiomyocyte proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Jiang,Lijuan Pei,Hongjie Zhang,Fenglian He,Yuhan Min,Xinhang Li,Ke Wei

Nature 631:424-431 PubMed38926571

2024

An intermediate Rb-E2F activity state safeguards proliferation commitment.

Applications

Unspecified application

Species

Unspecified reactive species

Yumi Konagaya,David Rosenthal,Nalin Ratnayeke,Yilin Fan,Tobias Meyer
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com