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AB47474

Anti-Rb (phospho S795) antibody

4

(2 Reviews)

|

(12 Publications)

Rabbit Polyclonal RB phospho S795 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human RB1 phospho S795 aa 750-850.

View Alternative Names

Retinoblastoma-associated protein, p105-Rb, p110-RB1, pRb, pp110, Rb, RB1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S795) antibody (AB47474)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S795) antibody (AB47474)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue in the presence (right) and absence (left) of synthetic peptide, using ab47474 at a 1/50 dilution.

Western blot - Anti-Rb (phospho S795) antibody (AB47474)
  • WB

Unknown

Western blot - Anti-Rb (phospho S795) antibody (AB47474)

All lanes:

Western blot - Anti-Rb (phospho S795) antibody (ab47474) at 1/500 dilution

Lane 1:

Extracts from K562 cells treated with 10% serum after 48 hours of starvation at 30 µg

Lane 2:

Extracts from K562 cells at 30 µg

Predicted band size: 106 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, IHC-P, ELISA

applications

Immunogen

Synthetic Peptide within Human RB1 phospho S795 aa 750-850. The exact immunogen used to generate this antibody is proprietary information.

P06400

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Retinoblastoma protein (Rb) also known as pRb is an important regulatory protein with a molecular weight of approximately 110 kDa. It is mainly expressed in the nucleus of cells. Rb functions as a tumor suppressor by controlling the cell cycle progression from G1 to S phase. Rb becomes active when it is dephosphorylated allowing it to bind and inhibit E2F transcription factors consequently preventing the transcription of genes essential for S phase entry.
Biological function summary

Retinoblastoma protein influences cellular proliferation differentiation and apoptosis. It acts within a larger protein complex modulating various cellular responses. When functional Rb halts uncontrolled cell division important for maintaining normal tissue homeostasis. In its phosphorylated state known as phospho-Rb or phospho-Rb E182 it loses its regulatory capabilities which can lead to unrestrained cell cycle progression.

Pathways

Several involve the retinoblastoma protein. One key pathway is the p53 pathway which Rb interacts with to influence cellular outcomes like cell-cycle arrest and apoptosis. Rb cooperates with proteins like p21 to implement these processes. Additionally it is involved in the cyclin D-dependent kinase 4 (CDK4) pathway which modulates its phosphorylation state influencing binding interactions and cell cycle control.

Dysregulation of retinoblastoma protein is commonly associated with cancer particularly retinoblastoma and breast cancer. In retinoblastoma mutations in the Rb gene directly lead to uncontrolled cell proliferation due to the absence of functional Rb protein. Additionally Rb's connection to the E2F family of transcription factors can become disrupted contributing to oncogenesis in other cancers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed : 10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed : 10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed : 10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed : 10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed : 15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).. (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
See full target information RB1 phospho S795
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

British journal of cancer 131:444-456 PubMed38890443

2024

CDK4/6 inhibitors dephosphorylate RNF26 to stabilize TSC1 and increase the sensitivity of ccRCC to mTOR inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Xinlin Liu,Wei Li,Lu Yi,Jianxi Wang,Wentao Liu,Hongtao Cheng,Shangqing Ren

MedComm 3:e136 PubMed35711853

2022

visualization of fluorescence reflecting CDK4 activity in a breast cancer mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Yang Gao,Rui-Qin Yang,Kang-Liang Lou,Yong-Ying Dang,Yuan-Yuan Dong,Yue-Yang He,Wen-He Huang,Min Chen,Guo-Jun Zhang

Histochemistry and cell biology 158:193-198 PubMed35551458

2022

Evidence of cell cycle re-entry in post-mitotic, terminally differentiated feline neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Wisnet,Christoph H F Payer,Barbara Bauder,Angelika Url

Experimental neurology 320:113003 PubMed31260658

2019

Astrogliosis inhibition attenuates hydrocephalus by increasing cerebrospinal fluid reabsorption through the glymphatic system after germinal matrix hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Ding,Tongyu Zhang,Guangyong Wu,Devin W McBride,Ningbo Xu,Damon W Klebe,Yiting Zhang,Qian Li,Jiping Tang,John H Zhang

Cellular physiology and biochemistry : internation 51:2324-2340 PubMed30537738

2018

Circular RNA circPVT1 Promotes Proliferation and Invasion Through Sponging miR-125b and Activating E2F2 Signaling in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuyuan Li,Zenglei Zhang,Hua Jiang,Qiang Li,Ruliang Wang,Hongliang Pan,Yingying Niu,Fenghai Liu,Hongmei Gu,Xingjun Fan,Jinxia Gao

Cancer cell international 18:116 PubMed30123094

2018

Circ-ZEB1.33 promotes the proliferation of human HCC by sponging miR-200a-3p and upregulating CDK6.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhua Gong,Jinzhong Mao,Di Wu,Xuemei Wang,Long Li,Liang Zhu,Rong Song

Nature communications 8:1995 PubMed29222471

2017

Spatial competition constrains resistance to targeted cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Katarina Bacevic,Robert Noble,Ahmed Soffar,Orchid Wael Ammar,Benjamin Boszonyik,Susana Prieto,Charles Vincent,Michael E Hochberg,Liliana Krasinska,Daniel Fisher

Scientific reports 7:13429 PubMed29044141

2017

Cdk2 strengthens the intra-S checkpoint and counteracts cell cycle exit induced by DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Katarina Bačević,Gérald Lossaint,Thiziri Nait Achour,Virginie Georget,Daniel Fisher,Vjekoslav Dulić

Journal of neuroscience research 95:1582-1601 PubMed27862183

2016

The antiviral cytokine interferon-gamma restricts neural stem/progenitor cell proliferation through activation of STAT1 and modulation of retinoblastoma protein phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Apurva Kulkarni,Taylor J Scully,Lauren A O'Donnell

Nucleic acids research 43:e150 PubMed26250117

2015

Simultaneous measurement of passage through the restriction point and MCM loading in single cells.

Applications

WB

Species

Human

T W Håland,E Boye,T Stokke,B Grallert,R G Syljuåsen
View all publications
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