Name: Anti-Rb (phospho S807) antibody [EPR17732]
SKU: ab184796
Rating: 5 (2 reviews)

Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) is a rabbit monoclonal antibody detecting Rb in Western Blot, IHC-P, ICC/IF, Dot Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

- Over 20 publications

Related conjugates and formulations

ab277935
Conjugated
Alexa Fluor® 594 Anti-Rb (phospho S807) antibody [EPR17732]
ab214949
Conjugated
Alexa Fluor® 488 Anti-Rb (phospho S807) antibody [EPR17732]
ab280855
Conjugated
Alexa Fluor® 555 Anti-Rb (phospho S807) antibody [EPR17732]
ab215530
Carrier free
Anti-Rb (phospho S807) antibody [EPR17732] - BSA and Azide free
ab215947
Conjugated
Alexa Fluor® 647 Anti-Rb (phospho S807) antibody [EPR17732]

Images

Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (AB184796), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Dot	WB	ICC/IF	IHC-P
Human	Expected	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Tested
Rat	Expected	Tested	Expected	Tested
Synthetic peptide	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

See full target information RB1 phospho S807

Function

Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Alternative names

Retinoblastoma-associated protein, p105-Rb, p110-RB1, pRb, pp110, Rb, RB1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR17732
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Dot Blot in Human, Mouse, Rat samples.

What is the molecular weight of Rb?

Anti-Rb (phospho S807) [EPR17732] (ab184796) specifically detects a band for Rb (UniProt: P06400) at a molecular weight of 106kDa.

Trusted by the scientific community

Anti-Rb (phospho S807) [EPR17732] (ab184796) was first used in a scientific publication in 2015 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR17732] also available for your convenience:
ab184796, Alexa Fluor® 488 - ab214949, Carrier free - ab215530, Alexa Fluor® 647 - ab215947, Alexa Fluor® 594 - ab277935, Alexa Fluor® 555 - ab280855

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Retinoblastoma protein (Rb) also known as pRb is an important regulatory protein with a molecular weight of approximately 110 kDa. It is mainly expressed in the nucleus of cells. Rb functions as a tumor suppressor by controlling the cell cycle progression from G1 to S phase. Rb becomes active when it is dephosphorylated allowing it to bind and inhibit E2F transcription factors consequently preventing the transcription of genes essential for S phase entry.

Biological function summary

Retinoblastoma protein influences cellular proliferation differentiation and apoptosis. It acts within a larger protein complex modulating various cellular responses. When functional Rb halts uncontrolled cell division important for maintaining normal tissue homeostasis. In its phosphorylated state known as phospho-Rb or phospho-Rb E182 it loses its regulatory capabilities which can lead to unrestrained cell cycle progression.

Pathways

Several involve the retinoblastoma protein. One key pathway is the p53 pathway which Rb interacts with to influence cellular outcomes like cell-cycle arrest and apoptosis. Rb cooperates with proteins like p21 to implement these processes. Additionally it is involved in the cyclin D-dependent kinase 4 (CDK4) pathway which modulates its phosphorylation state influencing binding interactions and cell cycle control.

Associated diseases and disorders

Dysregulation of retinoblastoma protein is commonly associated with cancer particularly retinoblastoma and breast cancer. In retinoblastoma mutations in the Rb gene directly lead to uncontrolled cell proliferation due to the absence of functional Rb protein. Additionally Rb's connection to the E2F family of transcription factors can become disrupted contributing to oncogenesis in other cancers.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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14 product images

Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/20000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) serum starved for 44 h. Whole cell lysates at 15 µg
Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) serum starved for 24 h. Then 10% FBS incubated for 20 h. Whole cell lysates at 15 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) serum starved for 24 h. Then 10% FBS incubated for 20 h. Whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 10s
Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells, Serum starved and non-starved, labeling Rb (phospho S807) with ab184796 at 1/200 dilution followed by Goat anti-Rabbit secondary IgG AlexaFluor®488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing positive staining on MCF7 cells. The number of positive cells increased after treatment with FBS (fetal bovine serum) for 48 hours, then decreased after Lambda Protein Phosphatase treatment (31℃ for 2 hours ).
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/20000 dilution
Lane 1: K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates at 10 µg
Lane 2: K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates serum starved for 2 days at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3min
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Rb (phospho S807) Western blot staining using rabbit Anti-Rb (phospho S807) antibody
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/5000 dilution
Lane 1: K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates at 10 µg
Lane 2: K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates treated with Lambda Phosphatase at 10 µg
Secondary
Lane 1: Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution
Lane 2: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 30s
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/1000 dilution
All lanes: L-929 (mouse connective tissue fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3min
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/2000 dilution
All lanes: Mouse embryo lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3min
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/2000 dilution
All lanes: C6 (rat glial tumor cell line) whole cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 30s
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/1000 dilution
All lanes: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nucleus staining on epithelial cells of Human colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Immunohistochemical analysis of paraffin-embedded human Squamous cells carcinoma of lung tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nucleus staining on squamous cells carcinoma of lung is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nucleus staining on lymphocytes of mouse spleen is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nucleus staining on lymphocytes of rat spleen is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Dot Blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Dot blot analysis of Rb (phospho S807) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab184796 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
Rb (phospho S807) western blot using anti-Rb (phospho S807) antibody [EPR17732] ab184796. Publication image and figure legend from Bouclier, C., Simon, M., et al., 2020, Theranostics, PubMed 32104498.

ab184796 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab184796 please see the product overview.
P2short inhibits CDK4 activity and NSCLC proliferation but does not block cell cycle arrest. A. p107Rb phosphorylation (Ser807/811) was determined by Western blot of A549 cell extracts prepared following treatment with Abemaciclib or P2short peptide. Actin levels were detected to control constant protein loading. Left panel: Rb phosphorylation after 24 and 48 h, following treatment with 20 µM Abemaciclib or P2short peptide. Right panel: quantification of the relative Rb phosphorylation/actin levels in after 24 h following treatment with 20 µM Ctrl stapled peptide or different µM concentrations of P2short peptide. B-E.Proliferation assays.B. A549 cells were treated with different concentrations of Ctrl stapled peptide, P2, P2shortA and Abemaciclib. C. A549 cells were treated with combined administrations of P2shortA peptide (10 µM) and Abemaciclib (500 nM) Bars are averages of three independent experiments (n=4/experiment) ± SD. Means were compared with t test, * p<0.05,** p<0.02, *** p<0.01. D-E. PC9, H358 and A549 cells were treated with different concentrations of P2shortA (D) or Abemaciclib (E). Results are presented as average of at least two independent experiments (n = 4/experiment) ± standard deviation. F. Flow cytometry of A549 cells treated with DMSO, 0.5 or 5 µM Abemaciclib (Abe) and/or 1 or 10 µM Ctrl stapled or P2shortA peptide for 24 h. Means were compared with t test, * p<0.2,** p<0.1, *** p<0.05.