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AB133446

Anti-Rb (phospho T826) antibody [EPR5351]

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(10 Publications)

Rabbit Recombinant Monoclonal RB phospho T826 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications.

View Alternative Names

Retinoblastoma-associated protein, p105-Rb, p110-RB1, pRb, pp110, Rb, RB1

3 Images
Immunoprecipitation - Anti-Rb (phospho T826) antibody [EPR5351] (AB133446)
  • IP

Lab

Immunoprecipitation - Anti-Rb (phospho T826) antibody [EPR5351] (AB133446)

Rb was immunoprecipitated from 0.35 mg Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg with ab133446 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg

Lane 2 : ab133446 IP in Raji whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab133446 in Raji whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Lower bands could be Rb fragments (PMID : 11420704)

All lanes:

Immunoprecipitation - Anti-Rb (phospho T826) antibody [EPR5351] (ab133446)

Predicted band size: 106 kDa

Observed band size: 110 kDa

false

Western blot - Anti-Rb (phospho T826) antibody [EPR5351] (AB133446)
  • WB

Unknown

Western blot - Anti-Rb (phospho T826) antibody [EPR5351] (AB133446)

All lanes:

Western blot - Anti-Rb (phospho T826) antibody [EPR5351] (ab133446) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Jurkat cell lysate treated with FBS. at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 106 kDa

Observed band size: 110 kDa

false

OI-RD Scanning - Anti-Rb (phospho T826) antibody [EPR5351] (AB133446)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Rb (phospho T826) antibody [EPR5351] (AB133446)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-Rb (phospho T826) antibody [EPR5351] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5351

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Retinoblastoma protein (Rb) also known as pRb is an important regulatory protein with a molecular weight of approximately 110 kDa. It is mainly expressed in the nucleus of cells. Rb functions as a tumor suppressor by controlling the cell cycle progression from G1 to S phase. Rb becomes active when it is dephosphorylated allowing it to bind and inhibit E2F transcription factors consequently preventing the transcription of genes essential for S phase entry.
Biological function summary

Retinoblastoma protein influences cellular proliferation differentiation and apoptosis. It acts within a larger protein complex modulating various cellular responses. When functional Rb halts uncontrolled cell division important for maintaining normal tissue homeostasis. In its phosphorylated state known as phospho-Rb or phospho-Rb E182 it loses its regulatory capabilities which can lead to unrestrained cell cycle progression.

Pathways

Several involve the retinoblastoma protein. One key pathway is the p53 pathway which Rb interacts with to influence cellular outcomes like cell-cycle arrest and apoptosis. Rb cooperates with proteins like p21 to implement these processes. Additionally it is involved in the cyclin D-dependent kinase 4 (CDK4) pathway which modulates its phosphorylation state influencing binding interactions and cell cycle control.

Dysregulation of retinoblastoma protein is commonly associated with cancer particularly retinoblastoma and breast cancer. In retinoblastoma mutations in the Rb gene directly lead to uncontrolled cell proliferation due to the absence of functional Rb protein. Additionally Rb's connection to the E2F family of transcription factors can become disrupted contributing to oncogenesis in other cancers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed : 10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed : 10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed : 10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed : 10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed : 15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).. (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
See full target information RB1 pT826
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature 631:424-431 PubMed38926571

2024

An intermediate Rb-E2F activity state safeguards proliferation commitment.

Applications

Unspecified application

Species

Unspecified reactive species

Yumi Konagaya,David Rosenthal,Nalin Ratnayeke,Yilin Fan,Tobias Meyer

Nature communications 14:7069 PubMed37923710

2023

Selective activator of human ClpP triggers cell cycle arrest to inhibit lung squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Lin Zhou,Tao Zhang,Yun Xue,Chuan Yue,Yihui Pan,Pengyu Wang,Teng Yang,Meixia Li,Hu Zhou,Kan Ding,Jianhua Gan,Hongbin Ji,Cai-Guang Yang

The Journal of biological chemistry 298:102513 PubMed36150501

2022

Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase UL97 is required for regulatory protein 14-3-3 binding and UL97 stability.

Applications

Unspecified application

Species

Unspecified reactive species

Satoko Iwahori,Angie C Umaña,Robert F Kalejta,Takayuki Murata

Cancers 14: PubMed35884401

2022

Selective CDK9 Inhibition by Natural Compound Toyocamycin in Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Somnath Pandey,Rahinatou Djibo,Anaïs Darracq,Gennaro Calendo,Hanghang Zhang,Ryan A Henry,Andrew J Andrews,Stephen B Baylin,Jozef Madzo,Rafael Najmanovich,Jean-Pierre J Issa,Noël J-M Raynal

Nature communications 12:4841 PubMed34404770

2021

ZNF768 links oncogenic RAS to cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Romain Villot,Audrey Poirier,Inan Bakan,Karine Boulay,Erlinda Fernández,Romain Devillers,Luciano Gama-Braga,Laura Tribouillard,Andréanne Gagné,Éma Duchesne,Danielle Caron,Jean-Sébastien Bérubé,Jean-Christophe Bérubé,Yan Coulombe,Michèle Orain,Yves Gélinas,Stéphane Gobeil,Yohan Bossé,Jean-Yves Masson,Sabine Elowe,Steve Bilodeau,Venkata Manem,Philippe Joubert,Frédérick A Mallette,Mathieu Laplante

Cancer cell 34:626-642.e8 PubMed30300583

2018

The Oncogenic Transcription Factor RUNX1/ETO Corrupts Cell Cycle Regulation to Drive Leukemic Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Martinez-Soria,Lynsey McKenzie,Julia Draper,Anetta Ptasinska,Hasan Issa,Sandeep Potluri,Helen J Blair,Anna Pickin,Asmida Isa,Paulynn Suyin Chin,Ricky Tirtakusuma,Daniel Coleman,Sirintra Nakjang,Salam Assi,Victoria Forster,Mojgan Reza,Ed Law,Philip Berry,Dorothee Mueller,Cameron Osborne,Alex Elder,Simon N Bomken,Deepali Pal,James M Allan,Gareth J Veal,Peter N Cockerill,Christian Wichmann,Josef Vormoor,Georges Lacaud,Constanze Bonifer,Olaf Heidenreich

Endocrine journal 64:1115-1123 PubMed28855436

2017

Effects of sorafenib and an adenylyl cyclase activator on in vitro growth of well-differentiated thyroid cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aya Sawa,Tomohiro Chiba,Jun Ishii,Hiroyuki Yamamoto,Hisato Hara,Hiroshi Kamma

Oncogene 36:4349-4361 PubMed28368408

2017

JNKs function as CDK4-activating kinases by phosphorylating CDK4 and p21.

Applications

WB

Species

Unspecified reactive species

B Colleoni,S Paternot,J M Pita,X Bisteau,K Coulonval,R J Davis,E Raspé,P P Roger

Clinical cancer research : an official journal of 22:5514-5526 PubMed27252418

2016

High CDK6 Protects Cells from Fulvestrant-Mediated Apoptosis and is a Predictor of Resistance to Fulvestrant in Estrogen Receptor-Positive Metastatic Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Carla L Alves,Daniel Elias,Maria Lyng,Martin Bak,Tove Kirkegaard,Anne E Lykkesfeldt,Henrik J Ditzel

The Journal of biological chemistry 290:19666-80 PubMed26100623

2015

Molecular Determinants for the Inactivation of the Retinoblastoma Tumor Suppressor by the Viral Cyclin-dependent Kinase UL97.

Applications

Unspecified application

Species

Unspecified reactive species

Satoko Iwahori,Morgan Hakki,Sunwen Chou,Robert F Kalejta
View all publications
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Product promise

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