AB244373

Anti-Rb2 p130 antibody

Name: Anti-Rb2 p130 antibody (ab244373) | Abcam
Brand: Abcam Limited
SKU: AB244373
Price: 630 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal p130 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBL2 aa 600 to C-terminus.

View Alternative Names

RB2, RBL2, Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2, pRb2, p130, RBR-2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 antibody (AB244373)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 antibody (AB244373)

Paraffin-embedded human tonsil tissue stained for Rb2 p130 using ab244373 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 antibody (AB244373)

Paraffin-embedded human cerebellum tissue stained for Rb2 p130 using ab244373 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 antibody (AB244373)

Paraffin-embedded human endometrium tissue stained for Rb2 p130 using ab244373 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 antibody (AB244373)

Paraffin-embedded human testis tissue stained for Rb2 p130 using ab244373 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Rb2 p130 antibody (AB244373)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rb2 p130 antibody (AB244373)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Rb2 p130 (green) using ab244373 at 4 μg/ml in ICC/IF.

Unknown

Western blot - Anti-Rb2 p130 antibody (AB244373)

All lanes:

Western blot - Anti-Rb2 p130 antibody (ab244373) at 0.4 µg/mL

All lanes:

MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate

Predicted band size: 128 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RBL2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q08999

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rb2 p130 also known as Retinoblastoma-like protein 2 functions as an important regulator of cell cycle progression. It has a molecular weight of about 130 kDa and members of the retinoblastoma (Rb) protein family which also includes p107 and RB1. These proteins help control the transition from the G1 to S phase during the cell cycle. Rb2 p130 is widely expressed in various tissues particularly in quiescent cells where it plays a role in maintaining cells in the G0 phase.

Biological function summary

The protein acts as a fundamental component of the DREAM (DP RB-like E2F and MuvB) complex which plays an important role in repressing the transcription of E2F target genes. This repression ensures the proper regulation of cell proliferation and differentiation. The protein contributes to maintaining genomic stability by helping to control the entry of cells into the DNA synthesis phase thereby acting as a tumor suppressor.

Pathways

Rb2 p130 is heavily involved in the cell cycle control and proliferation pathways. It associates with the E2F family of transcription factors which are critical in transcription regulation of genes important for cell cycle progression. It modulates activities within the p53 pathway as well another pathway important for cell cycle arrest and apoptosis. This relation highlights its interplay with proteins like cyclin-dependent kinases (CDKs) which regulate cell cycle transitions through phosphorylation events.

The protein has been linked to several cancer types due to its role as a tumor suppressor. Disruptions in its expression or function can lead to unchecked cellular division paving the way for tumorigenesis. Mutations or alterations in the Rb pathway involving Rb2 p130 RB1 and p107 can contribute to the development of cancers such as retinoblastoma and lung carcinoma. The involvement of these proteins signifies their importance in maintaining controlled cell division with impairments correlating strongly to cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

See full target information RBL2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 6:129 PubMed33785736

2021

PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Wook Kang,Zhiyan Zhan,Guangzhen Ji,Youzhou Sang,Daohong Zhou,Yanxin Li,Haizhong Feng,Tao Cheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rb2 p130 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 antibody (AB244373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 antibody (AB244373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 antibody (AB244373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 antibody (AB244373)

Immunocytochemistry/ Immunofluorescence - Anti-Rb2 p130 antibody (AB244373)

Western blot - Anti-Rb2 p130 antibody (AB244373)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise